Home / SF20/MYDGF / Human SF20/MYDGF Antibody

Human SF20/MYDGF Antibody

Catalog #: AF1147
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1147
AF1147-SP
Detection of Human SF20/MYDGF by Western Blot.
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Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SF20/MYDGF Antibody Summary

Species Reactivity
Human
Specificity
Detects human SF20/MYDGF in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse SF20/MYDGF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SF20/MYDGF
Val32-Leu173
Accession # Q969H8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
25 µg/mL
DU145 human prostate carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SF20/MYDGF antibody by Western Blot. View Larger

Detection of Human SF20/MYDGF by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SF20/MYDGF Antigen Affinity-purified Antibody (Catalog # AF1147) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SF20/MYDGF at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western View Larger

Detection of Human SF20/MYDGF by Simple WesternTM. Simple Western lane view shows lysates of DU145 human prostate carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SF20/MYDGF at approximately 22 kDa (as indicated) using 25 µg/mL of Goat Anti-Human SF20/MYDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1147). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SF20/MYDGF

Human SF20 (Stromal cell-derived growth factor SF20, C19orf10, MYDGF1, Myeloid-derived growth factor1 also formerly known as IL25) is a 15 kDa (predicted)  bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction. SF20 stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1 mediated signaling pathway. It is thought to inhibit cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway and is involved in endothelial cell proliferation and angiogenesis. Human SF20 shares 87% aa identity with mouse SF20.              

Long Name
Stromal Cell-derived Growth Factor SF20
Entrez Gene IDs
56005 (Human); 28106 (Mouse)
Alternate Names
C19orf10; chromosome 19 open reading frame 10; EUROIMAGE1875335; IL25; IL27; IL27w; interleukin 27 working designation; interleukin-25; Ly6elg; MYDGF; myeloid-derived growth factor; R33729_1; SF20; Stromal cell-derived growth factor SF20; UPF0556 protein C19orf10

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Citation for Human SF20/MYDGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Myeloid-derived growth factor is a resident endoplasmic reticulum protein
    Authors: V Bortnov, DS Annis, FJ Fogerty, KT Barretto, KB Turton, DF Mosher
    J. Biol. Chem., 2018-06-28;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

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