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Mouse Sortilin Antibody

Catalog #: AF2934
18 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2934
AF2934-SP
Detection of Mouse Sortilin by Western Blot.
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Citations (18)
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Mouse Sortilin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Sortilin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross‑reactivity with recombinant human Sortilin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Sortilin
Gly76-Asn753
Accession # Q6PHU5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1.5-6 µg/mL of this antibody will block 50% of the binding of 200 ng/mL of Recombinant Human beta -NGF (Catalog # 256-GF) to immobilized Recombinant Mouse Sortilin (Catalog # 2934-ST) coated at 4 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Sortilin antibody by Western Blot. View Larger

Detection of Mouse Sortilin by Western Blot. Western blot shows lysates of mouse brain (total) tissue, mouse brain (cerebellum) tissue, and mouse brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2934) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Sortilin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Sortilin antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

Sortilin in Mouse Brain. Sortilin was detected in perfusion fixed frozen sections of mouse brain (rostral ventral medulla) using Goat Anti-Mouse Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2934) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse Sortilin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Sortilin by Simple WesternTM. Simple Western lane view shows lysates of mouse brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for Sortilin at approximately 122 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2934) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sortilin

Sortilin (neurotensin receptor 3, glycoprotein 95) is a 95 kDa Type I transmembrane monomeric glycoprotein that is one of five known members of the mammalian vacuolar protein sorting 10p domain (Vps10p-D) family of sorting receptors (1, 2). Mouse preprosortilin is processed by signal sequence cleavage followed by propeptide cleavage at a furin recognition site. The cationic propeptide exhibits pH-dependent high affinity binding that blocks the Sortilin ligand binding site both pre‑ and post-cleavage (3). The extracellular/luminal sequence comprises the Vps10p domain, including 10 conserved cysteines (10CC) essential for ligand binding (2). The cytoplasmic domain sorting motifs confer all trafficking during synthesis, targeting to lysosomes, endocytosis and Golgi-endosome transport; as little as 10% may be found on the cell surface (4). Mature mouse Sortilin shares 98% amino acid (aa) identity with rat, and 91% aa identity with human and canine sortilin. During murine development, sortilin is mainly expressed in the nervous system (5), where it is a receptor for neuropeptides including neurotensin, nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) (6‑9). ProNGF (or the NGF propeptide alone) binds sortilin with a much higher affinity (KD ~5-8 nM) than does mature NGF (KD ~90 nM). The complex of sortilin, pro‑NGF and the receptor p75ntr results in endocytosis of proNGF and induction of apoptosis (7). Similar results have been obtained with proBDNF and BDNF (8‑9). Sortilin is expressed in other tissues including testis, skeletal muscle and fat (1, 10). It is essential and sufficient for biogenesis of Glut4 storage vesicles necessary for insulin responsiveness in adipocytes (10). Sortilin also binds lipoprotein lipase (11), apoE (2) and RAP (1, 11). Binding is competitive, indicating that although unrelated, targets likely bind the same site.

References
  1. Petersen, C.M. et al. (1997) J. Biol. Chem. 272:3599.
  2. Westergaard, U.B. et al. (2004) J. Biol. Chem. 279:50221. 
  3. Petersen, C.M. et al. (1998) EMBO J. 18:595. 
  4. Nielsen, M.S. et al. (2001) EMBO J. 20:2180. 
  5. Hermans-Borgmeyer, I. et al. (1999) Mol. Brain Res. 65:216.
  6. Mazella, J. et al. (1998) J. Biol. Chem. 273:26273.
  7. Nykjaer, A et al. (2004) Nature 427:843.
  8. Teng, H.K. et al. (2005) J. Neurosci. 25:5455.
  9. Chen, Z.-Y. et al. (2004) J. Neurosci. 25:6156.
  10. Shi, J and K.V. Kandror (2005) Dev. Cell 9:99.
  11. Nielsen, M.S. et al. (1999) J. Biol. Chem. 274:8832.
Entrez Gene IDs
6272 (Human); 20661 (Mouse)
Alternate Names
100 kDa NT receptor; Glycoprotein 95; Gp95; Gp95LDLCQ6; Neurotensin receptor 3; NT3NTR3; Ntr3; SORT1; sortilin 1; Sortilin

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Citations for Mouse Sortilin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. A single injection of gain-of-function mutant PCSK9 adeno-associated virus vector induces cardiovascular calcification in mice with no genetic modification.
    Authors: Goettsch C, Hutcheson JD, Hagita S et al.
    Atherosclerosis
  2. Neuron-specific gene NSG1 binds to and positively regulates sortilin ectodomain shedding via a metalloproteinase-dependent mechanism
    Authors: Overby, M;Serrano-Rodriguez, A;Dadras, S;Christiansen, AK;Ozcelik, G;Lichtenthaler, SF;Weick, JP;Müller, HK;
    The Journal of biological chemistry
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  3. Sortilin Is Upregulated in Osteoarthritis-Dependent Cartilage Calcification and Associated with Cellular Senescence
    Authors: Richter, E;Lohmann, CH;Dell'Accio, F;Goettsch, C;Bertrand, J;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Sortilin Inhibition Protects Neurons From Degeneration in the Diabetic Retina
    Authors: Jakobsen, TS;Østergaard, JA;Kjolby, M;Birch, EL;Bek, T;Nykjaer, A;Corydon, TJ;Askou, AL;
    Investigative ophthalmology & visual science
    Species: Mouse
    Sample Types:
    Applications: In Vivo
  5. Sortilins in the blood-brain barrier: impact on barrier integrity
    Authors: Andrea E. Nielsen, Morten S. Nielsen
    Neural Regeneration Research
  6. Sortilin Modulates Schwann Cell Signaling and Remak Bundle Regeneration Following Nerve Injury
    Authors: M Ulrichsen, NP Gonçalves, S Mohseni, S Hjæresen, TL Lisle, S Molgaard, NK Madsen, OM Andersen, ÅF Svenningse, S Glerup, A Nykjær, CB Vægter
    Frontiers in Cellular Neuroscience, 2022-05-11;16(0):856734.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. ApoE4 disrupts interaction of sortilin with fatty acid-binding protein 7 essential to promote lipid signaling
    Authors: Antonino Asaro, Rishabhdev Sinha, Magda Bakun, Oleksandra Kalnytska, Anne-Sophie Carlo-Spiewok, Tymon Rubel et al.
    Journal of Cell Science
  8. Wnt/&beta-catenin signaling regulates brain-derived neurotrophic factor release from spinal microglia to mediate HIV1 gp120-induced neuropathic pain
    Authors: X Zhou, L Tao, M Zhao, S Wu, E Obeng, D Wang, W Zhang
    Mol Pain, 2020-01-01;16(0):1744806920922.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Sortilin gates neurotensin and BDNF signaling to control peripheral neuropathic pain
    Authors: M Richner, LT Pallesen, M Ulrichsen, ET Poulsen, TH Holm, H Login, A Castonguay, LE Lorenzo, NP Gonçalves, OM Andersen, K Lykke-Hart, JJ Enghild, LCB Rønn, IJ Malik, Y De Koninck, OJ Bjerrum, CB Vægter, A Nykjær
    Sci Adv, 2019-06-19;5(6):eaav9946.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. Prions amplify through degradation of the VPS10P sorting receptor sortilin
    Authors: K Uchiyama, M Tomita, M Yano, J Chida, H Hara, NR Das, A Nykjaer, S Sakaguchi
    PLoS Pathog., 2017-06-30;13(6):e1006470.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  11. Impaired prosaposin lysosomal trafficking in frontotemporal lobar degeneration due to progranulin mutations
    Authors: X Zhou, L Sun, O Bracko, JW Choi, Y Jia, AL Nana, OA Brady, JCC Hernandez, N Nishimura, WW Seeley, F Hu
    Nat Commun, 2017-05-25;8(0):15277.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Spatiotemporal patterns of sortilin and SorCS2 localization during organ development
    Authors: Simon Boggild, Simon Molgaard, Simon Glerup, Jens Randel Nyengaard
    BMC Cell Biology
  13. Organ of Corti explants direct tonotopically graded morphology of spiral ganglion neurons in vitro
    Authors: Felicia L. Smith, Robin L. Davis
    Journal of Comparative Neurology
  14. Sortilin and SorLA Display Distinct Roles in Processing and Trafficking of Amyloid Precursor Protein
    Authors: Camilla Gustafsen, Simon Glerup, Lone Tjener Pallesen, Ditte Olsen, Olav M. Andersen, Anders Nykjær et al.
    The Journal of Neuroscience
  15. Neurotrophic actions initiated by proNGF in adult sensory neurons may require peri-somatic glia to drive local cleavage to NGF.
    J. Neurochem., 2012-06-12;122(3):523-36.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  16. Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling.
    Authors: Vaegter CB, Jansen P, Fjorback AW
    Nat. Neurosci., 2010-11-21;14(1):54-61.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  17. From noncoding variant to phenotype via SORT1 at the 1p13 cholesterol locus.
    Authors: Musunuru K, Strong A, Frank-Kamenetsky M
    Nature, 2010-08-05;466(7307):714-9.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  18. Antagonism of p66shc by melanoma inhibitory activity.
    Authors: Kasuno K, Naqvi A, Dericco J, Yamamori T, Santhanam L, Mattagajasingh I, Yang S, Meyskens FL, Bosserhoff AK, Irani K
    Cell Death Differ., 2007-04-13;14(8):1414-21.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot

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