Human GFAP NL557 Affinity Purified Polyclonal Ab

Detection of Human GFAP by Immunocytochemistry/Immunofluorescence Differentiation of K-HME cells. Osteoclast: A.i. Phase contrast, Laminin, 20x, A.ii. Tartrate resistant acid phosphatase (TRAP). The bright pink is indicative of TRAP activity. Chondrocyte: B.i. Phase contrast, 40x, ii. Alcian blue staining. The blue color is due to the glycosaminoglycans synthesized by chondrocytes. iii. IHC anti-collagen II, iv. IHC anti-collagen X. Adipocyte: C.i. Phase contrast, ii. Oil Red O staining. Muscle: D.i. Phase contrast, Collagen IV, 20x; ii. MyoD immunohistochemistry. MyoD is an immature muscle marker. Neural: E.i. immunofluorescence beta -III tubulin, glial fibrillary acid protein (GFAP) and nestin. beta -III tubulin is expressed by differentiated neurons. GFAP is expressed by mature astrocytes and distinguishes astrocytes from other glial cells during development. Nestin expression marks stem cells of the central nervous system. ii. IHC anti-NeuN. Melanocyte: F. Immunohistochemistry using anti-MART-1 (anti-Melan-A; melanocyte differentiation antigen), 20x. Positive and negative controls stained appropriately, A-F. Image collected and cropped by CiteAb from the following publication (https://bmcmolcellbiol.biomedcentral.com/articles/10.1186/1471-2121-15-20), licensed under a CC-BY license. Not internally tested by R&D Systems.