Human Collagen XXIII alpha 1 Antibody Summary
Glu111-Lys540
Accession # NP_775736
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Collagen XXIII alpha 1 by Western Blot. Western blot shows lysates of NCI-H460 human large cell lung carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human Collagen XXIII a1 Monoclonal Antibody (Catalog # MAB4165) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Collagen XXIII a1 at approximately 75 and 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Collagen XXIII alpha 1 in Human Adenocarcinoma. Collagen XXIII a1 was detected in immersion fixed paraffin-embedded sections of human adenocarcinoma using Human Collagen XXIII a1 Monoclonal Antibody (Catalog # MAB4165) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Collagen XXIII alpha 1
Collagen XXIII alpha 1 (COL23A1) is an epithelial cell type 2 transmembrane glycoprotein with multiple collagen like domains in its extracellular region. Intracellular furin mediated cleavage of the 68 kDa molecule generates a 58 kDa shed fragment that forms disulfide linked trimers. An alternately spliced isoform consists of nearly 300 amino acids of the membrane proximal extracellular domain (ECD), flanked by substituted sequences. COL23A1 is upregulated in many transformed cell lines and serves as an indicator for tumor aggressiveness in prostate cancer. Within the ECD, human COL23A1 shares 89% aa sequence identity with mouse and rat COL23A1.
Product Datasheets
Citations for Human Collagen XXIII alpha 1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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The Oncogenic Role of COL23A1 in Clear Cell Renal Cell Carcinoma
Authors: Fujiang Xu, Kun Chang, Jian Ma, Yuanyuan Qu, Huyang Xie, Bo Dai et al.
Scientific Reports
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Elevated ectodomain of type 23 collagen is a novel biomarker of the intestinal epithelium to monitor disease activity in ulcerative colitis and Crohn's disease
Authors: T. Manon‐Jensen, S. Sun, M. Lindholm, V. Domislović, P. Giuffrida, M. Brinar et al.
United European Gastroenterology Journal
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A role for collagen XXIII in cancer cell adhesion, anchorage-independence and metastasis.
Authors: Spivey KA, Chung I, Banyard J
Oncogene, 2011-10-03;31(18):2362-72.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
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