Recombinant Mouse PD-1 Fc Chimera Protein, CF Summary
Product Specifications
| Mouse PD-1 (Leu25-Gln167) Accession # Q02242 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1021-PD
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-HCL, NaCl and EDTA with Trehalose. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
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When Recombinant Mouse PD-1 Fc Chimera (Catalog # 1021-PD) is coated at 1 µg/mL, Recombinant Mouse B7-H1/PD-L1 Fc Chimera (Catalog # 1019-B7) binds with an ED50 of 0.16-0.8 µg/mL.
Reconstitution Calculator
Background: PD-1
Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors (1). Other members of this family include CD28, CTLA-4, ICOS, and BTLA (2-5). Mature mouse PD-1 consists of a 149 amino acid (aa) extracellular region (ECD) with one immunoglobulin-like V-type domain, a 21 aa transmembrane domain, and a 98 aa cytoplasmic region. The mouse PD-1 ECD shares 65% aa sequence identity with the human PD-1 ECD. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important for mediating PD-1 signaling. PD-1 acts as a monomeric receptor and interacts in a 1:1 stoichiometric ratio with its ligands PD-L1 (B7-H1) and PD-L2 (B7-DC) (6, 7). PD‑1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and also on nonhematopoietic cells such as lung endothelial cells and hepatocytes (8, 9). Ligation of PD-L1 with PD-1 induces
co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion (10). Thus, the PD-1:PD-L1 interaction is a key regulator of the threshold of immune response and peripheral immune tolerance (11). Finally, blockade of the PD-1: PD-L1 interaction by either antibodies or genetic manipulation accelerates tumor eradication and shows potential for improving cancer immunotherapy (12, 13).
- Ishida, Y. et al. (1992) EMBO J. 11:3887.
- Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116.
- Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
- Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
- Watanabe, N et al. (2003) Nat. Immunol. 4:670.
- Zhang, X. et al. (2004) Immunity 20:337.
- Lázár-Molnár, E. et al. (2008) Proc. Natl. Acad. Sci. USA 105:10483.
- Nishimura, H et al. (1996) Int. Immunol. 8:773.
- Keir, M.E. et al. (2008) Annu. Rev. Immunol. 26:677.
- Butte, M.J. et al. (2007) Immunity 27:111.
- Okazaki, T. et al. (2013) Nat. Immunol. 14:1212.
- Iwai, Y. et al. (2002) Proc. Natl. Acad. Sci. USA 99: 12293.
- Nogrady, B. (2014) Nature 513:S10.
Citations for Recombinant Mouse PD-1 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Co-Targeting Luminal B Breast Cancer with S-Adenosylmethionine and Immune Checkpoint Inhibitor Reduces Primary Tumor Growth and Progression, and Metastasis to Lungs and Bone
Authors: A Mehdi, M Attias, A Arakelian, CA Piccirillo, M Szyf, SA Rabbani
Cancers, 2022-12-22;15(1):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The multi-specific VH-based Humabody CB213 co-targets PD1 and LAG3 on T cells to promote anti-tumour activity
Authors: CJ Edwards, A Sette, C Cox, B Di Fiore, C Wyre, D Sydoruk, D Yadin, P Hayes, S Stelter, PD Bartlett, M Zuazo, MJ Garcia-Gra, G Benedetti, S Fiaska, NR Birkett, Y Teng, C Enever, H Arasanz, A Bocanegra, L Chocarro, G Fernandez, R Vera, B Archer, I Osuch, M Lewandowsk, YM Surani, G Kochan, D Escors, J Legg, AJ Pierce
British Journal of Cancer, 2021-12-30;0(0):.
Species: Mouse
Sample Types: Protein
Applications: Bioassay -
Dual and Opposite Costimulatory Targeting with a Novel Human Fusion Recombinant Protein Effectively Prevents Renal Warm Ischemia Reperfusion Injury and Allograft Rejection in Murine Models
Authors: J Guiteras, L De Ramon, E Crespo, N Bolaños, S Barcelo-Ba, L Martinez-V, P Fontova, M Jarque, A Torija, O Bestard, D Resina, JM Grinyó, J Torras
International Journal of Molecular Sciences, 2021-01-26;22(3):.
Species: Rat
Sample Types: In Vivo
Applications: Surface Plasmon Resonance -
Long-Term Systemic Expression of a Novel PD-1 Blocking Nanobody from an AAV Vector Provides Antitumor Activity without Toxicity
Authors: N Silva-Pili, E Martisova, MC Ballestero, S Hervas-Stu, N Casares, G González-S, C Smerdou, L Vanrell
Biomedicines, 2020-12-02;8(12):.
Species: Human
Sample Types: Cell Culture Supernates, Recombinant Protein
Applications: Bioassay, ELISA Capture -
DNA-Based Delivery of Checkpoint Inhibitors in Muscle and Tumor Enables Long-Term Responses with Distinct Exposure
Authors: L Jacobs, E De Smidt, N Geukens, P Declerck, K Hollevoet
Mol. Ther., 2020-02-13;0(0):.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Standard -
Botulinum toxin A complex exploits intestinal M cells to enter the host and exert neurotoxicity.
Authors: Matsumura, Takuhiro, Sugawara, Yo, Yutani, Masahiro, Amatsu, Sho, Yagita, Hideo, Kohda, Tomoko, Fukuoka, Shin-Ich, Nakamura, Yutaka, Fukuda, Shinji, Hase, Koji, Ohno, Hiroshi, Fujinaga, Yukako
Nat Commun, 2015-02-17;6(0):6255.
Species: Mouse
Sample Types: Protein
Applications: Enzyme Assay -
Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses.
Authors: Butte MJ, Keir ME, Phamduy TB, Sharpe AH, Freeman GJ
Immunity, 2007-07-12;27(1):111-22.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
BTNL2, a butyrophilin/B7-like molecule, is a negative costimulatory molecule modulated in intestinal inflammation.
Authors: Arnett HA, Escobar SS, Gonzalez-Suarez E, Budelsky AL, Steffen LA, Boiani N, Zhang M, Siu G, Brewer AW, Viney JL
J. Immunol., 2007-02-01;178(3):1523-33.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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0.5 ug mPD-1-hIgG1 (Cat# 1021-PD-100) was added to cells expressing mPD-L1 mCherry. Binding was detected with anti-human 488 secondary antibody.
