Recombinant Human 2B4/CD244/SLAMF4 Fc Chimera Protein, CF

Catalog #: 1039-2B
1 Citation Datasheet / COA / SDS

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1039-2B-050

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All 2B4/CD244/SLAMF4 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human 2B4/CD244/SLAMF4 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized rh2B4/Fc Chimera at 5 µg/mL (100 µL/well) can bind rhCD48 with a linear range of 0.06‑4 µg/mL.

Source

Mouse myeloma cell line, NS0-derived human 2B4/CD244/SLAMF4 protein

Human 2B4 (Cys22 - Arg221) Accession # NP_057466	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Accession #

NP_057466

N-terminal Sequence
Analysis

Cys22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

48.8 kDa (monomer)

SDS-PAGE

81 kDa, reducing conditions

Product Datasheets

1039-2B

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1039-2B

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: 2B4/CD244/SLAMF4

2B4, also known as CD244 and SLAMF4, is a 66 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 protein family. SLAM family proteins have an extracellular domain (ECD) with two or four Ig-like domains and at least two cytoplasmic immunoreceptor tyrosine-based switch motifs (ITSMs). 2B4 interacts with CD48, while other SLAM family proteins interact homophilically (1 - 4). Mature human 2B4 consists of a 208 amino acid (aa) ECD with two Ig-like domains, a 21 aa transmembrane segment, and a 120 aa cytoplasmic domain with four ITSMs (5, 6). Three additional splice variants of human 2B4 have deletions of the short region between the Ig-like domains, the second Ig-like domain, or a portion of the cytoplasmic tail. Within the ECD, human 2B4 shares 46% and 40% aa sequence identity with mouse and rat 2B4, respectively. The ECD of human 2B4 shares 17% - 24% aa sequence identity with comparable regions of human CD2 family members BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A, and SLAM. 2B4 is expressed on all NK cells, gamma δ T cells, monocytes, some CD4⁺ and CD8⁺ T cells, and some dendritic cells (7). CD48 mediates 2B4⁺ cell interactions with nearly all hematopoietic cell types, including cells of the same type (8 - 10). 2B4/CD48 signaling cooperates with other receptor systems to either promote or inhibit NK and CD8⁺ T cell activation (7 - 13). The inhibitory activities are distinct from those of MHC I restricted inhibitory NK cell receptors (12, 13). Ligation of 2B4 with antibodies or CD48 constructs can either directly trigger inhibitory signaling or disrupt an inhibitory interaction, leading to cellular activation (9, 12). The inhibitory effect is associated with the long form of 2B4, while the activation is associated with the short form (9, 14). 2B4 can also induce signaling through CD48 (10, 15).

References

Bhat, R. et al. (2006) J. Leukoc. Biol. 79:417.
Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
McNerney, M.E. et al. (2005) Mol. Immunol. 42:489.
Assarsson, E. et al. (2005) J. Immunol. 175:2045.
Boles, K.S. et al. (1999) Tissue Antigens 54:27.
Kubin, M.Z. et al. (1999) Eur. J. Immunol. 29:3466.
Nakajima, H. et al. (1999) Eur. J. Immunol. 29:1676.
Lee, K.M. et al. (2006) Blood 107:3181.
Mooney, J.M. et al. (2004) J. Immunol. 173:3953.
Assarsson, E. et al. (2004) J. Immunol. 173:174.
Bryceson, Y.T. et al. (2006) Blood 107:159.
Lee, K-M. et al. (2004) J. Exp. Med. 199:1245.
McNerney, M.E. et al. (2005) Blood 106:1337.
Schatzle, J.D. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3870.
Messmer, B. et al. (2006) J. Immunol. 176:4646.

Entrez Gene IDs

51744 (Human); 18106 (Mouse); 685808 (Rat); 102132700 (Cynomolgus Monkey)

Alternate Names

2B4; CD244 antigen; CD244 molecule, natural killer cell receptor 2B4; CD244; h2B4; NAIL; NAILNK cell activation-inducing ligand; natural killer cell receptor 2B4; NKR2B4; NKR2B4NK cell type I receptor protein 2B4,2B4CD244 natural killer cell receptor 2B4; Nmrk; SLAMF4; SLAMF4NK cell activation inducing ligand NAIL

Related Research Areas

Citation for Recombinant Human 2B4/CD244/SLAMF4 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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