Recombinant Human OX40 Ligand/TNFSF4 Protein, CF
Recombinant Human OX40 Ligand/TNFSF4 Protein, CF Summary
Product Specifications
| MHHHHHHHHHH | GGGSGGGSGGGS | IEGR | Human OX40 Ligand (Gln51-Leu183) Accession # Q6FGS4 |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1054-OX
| Formulation | Supplied as a 0.2 μm filtered solution in PBS. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
View Larger
Recombinant Human OX40 Ligand/TNFSF4 (Catalog # 1054-OX) induces IL-8 secretion in the HT1080 human fibrsarcoma cell line. The ED50 for this effect is 1-5 ng/mL.
View Larger
1 μg/lane of Recombinant Human OX40 Ligand/TNFSF4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing bands at 28-34 kDa.
Reconstitution Calculator
Background: OX40 Ligand/TNFSF4
OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Human OX40L cDNA encodes a 183 amino acids (aa) polypeptide with an amino-terminal cytoplasmic domain (aa 1¬23) and a carboxy¬terminal extracellular domain (aa 51-183). It shares 46% aa sequence identity with the mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Similarly to other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40 Ligand is one of the co¬stimulatory molecules in the immune system that includes B7, CD40 Ligand, CD30 Ligand, CD27 Ligand and 4¬1BB Ligand. Because OX40 appears as a late activation-induced T cell surface antigen, it has been speculated that the major function of OX40-OX40L interaction is to transmit a late co-stimulatory signal to promote the survival and proliferation of activated CD4+ T cells and prolong the immune response. Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression.
- Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757
- Baum, P.R. et al. (1994) EMBO J. 13:3992.
- AlShamkhani, A. et al. (1997) J. Biol. Chem. 272:5275.
- Kjaergaard, J. et al. (2000) Cancer Res. 60:5514.
Citations for Recombinant Human OX40 Ligand/TNFSF4 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
8
Citations: Showing 1 - 8
Filter your results:
Filter by:
-
CD106 in tumor-specific exhausted CD8+ T cells mediates immunosuppression by inhibiting TCR signaling
Authors: Naoi, Y;Morinaga, T;Nagasaki, J;Ariyasu, R;Ueda, Y;Yamashita, K;Zhou, W;Kawashima, S;Kawase, K;Honobe-Tabuchi, A;Ohnuma, T;Kawamura, T;Umeda, Y;Kawahara, Y;Nakamura, Y;Kiniwa, Y;Yamasaki, O;Fukushima, S;Kawazu, M;Suzuki, Y;Nishikawa, H;Hanazawa, T;Ando, M;Inozume, T;Togashi, Y;
Cancer research
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A multivariate modeling framework to quantify immune checkpoint context-dependent stimulation on T cells
Authors: L Karpf, C Trichot, L Faucheux, I Legbre, M Grandclaud, C Lahoute, H Mattoo, B Pasquier, V Soumelis
Cell Discovery, 2022-01-04;8(1):1.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Restoration of T Cell function in multi-drug resistant bacterial sepsis after interleukin-7, anti-PD-L1, and OX-40 administration
Authors: LK Thampy, KE Remy, AH Walton, Z Hong, K Liu, R Liu, V Yi, CD Burnham, RS Hotchkiss
PLoS ONE, 2018-06-26;13(6):e0199497.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease
Authors: AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle
MAbs, 2018-03-05;0(0):1-43.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR -
Production and characterization of a novel site-specific-modifiable anti-OX40-receptor single-chain variable fragment for targeted drug delivery
Authors: A Tanabe, K Nakano, M Nakakido, S Nagatoishi, Y Tanaka, K Tsumoto, K Uchimaru, T Watanabe
Biochem. Biophys. Res. Commun., 2018-01-09;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Murine insulin growth factor-like (IGFL) and human IGFL1 proteins are induced in inflammatory skin conditions and bind to a novel tumor necrosis factor receptor family member, IGFLR1.
Authors: Lobito AA, Ramani SR, Tom I, Bazan JF, Luis E, Fairbrother WJ, Ouyang W, Gonzalez LC
J. Biol. Chem., 2011-03-31;286(21):18969-81.
Species: Human
Sample Types:
Applications: Surface Plasmon Resonance -
Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.
Authors: Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S
Int. Immunol., 2008-08-13;20(10):1321-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Mapping the domains of CD134 as a functional receptor for feline immunodeficiency virus.
Authors: Willett BJ, McMonagle EL, Bonci F, Pistello M, Hosie MJ
J. Virol., 2006-08-01;80(15):7744-7.
Species: Feline
Sample Types: Whole Cells
Applications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human OX40 Ligand/TNFSF4 Protein, CF
Average Rating: 5 (Based on 4 Reviews)
Have you used Recombinant Human OX40 Ligand/TNFSF4 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
