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Recombinant Human Cathepsin V Protein, CF

Catalog #: 1080-CY
7 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1080-CY-010
R&D Systems Recombinant Proteins and Enzymes
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Citations (7)
FAQs
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Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Cathepsin V Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC (Catalog # ES008). The specific activity is >400 pmol/min/µg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human Cathepsin V protein
Val18-Val334, with a C-terminal 10-His tag
Accession #
O60911
N-terminal Sequence
Analysis
Val18
Structure / Form
Pro form
Predicted Molecular Mass
37 kDa
SDS-PAGE
40 kDa, reducing conditions

Product Datasheets

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1080-CY

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1080-CY

Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Assay Procedure

Materials
  • Assay Buffer: 25 mM Sodium Acetate, 0.1 M NaCl, 5 mM DTT, pH 5.5
  • Recombinant Human Cathepsin V (rhCathepsin V) (Catalog # 1080-CY)
  • Substrate: Z-Leu-Arg-AMC ((Catalog # ES008), 2 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhCathepsin V to 1 µg/mL in Assay Buffer.
  2. Dilute Substrate to 20 µM in Assay Buffer.
  3. Load into a black well plate 50 µL of 1 µg/mL rhCathepsin V and start the reaction by adding 50 µL of 20 µM Substrate.
  4. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes.
  5. Calculate specific activity:

         Specific Activity (pmol/min/µg) =

    		 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
    amount of enzyme (µg)

         *Adjusted for Substrate Blank

         **Derived using calibration standard 7-Amino, 4-Methyl Coumarin (AMC) (Sigma, Catalog # A-9891).

Per Well:
  • rhCathepsin V: 0.050 μg
  • Substrate: 10 µM
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cathepsin V

Cathepsin V (also known as Cathepsin L2 and U) is a cysteine protease of the papain family (1). It is expressed as a proenzyme in lysosomes, which can be autocatalytically converted to the mature form at pH 4.0 (2). Cathepsin V shows high amino acid identity with Cathepsin L (77% for the proenzymes and 80% for the mature enzymes) (3). However, Cathepsin V is specifically expressed in the thymus, testis, and corneal epithelium whereas Cathepsin L is expressed throughout the body.

References
  1. Turk, V. et al. (2001) EMBO J. 20:4629.
  2. Bromme, D. et al. (1999) Biochemistry 38:2377.
  3. Somoza, J.R. et al. (2000) Biochemistry 39:12543.
Entrez Gene IDs
1515 (Human)
Alternate Names
Cathepsin L2; Cathepsin U; Cathepsin V; CATL2; CTSL2; CTSUcathepsin L2, preproprotein; CTSVMGC125957; EC 3.4.22; EC 3.4.22.43

Citations for Recombinant Human Cathepsin V Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Nonclinical and clinical pharmacological characterization of the potent and selective cathepsin K inhibitor MIV-711
    Authors: E Lindström, B Rizoska, I Henderson, Y Terelius, M Jerling, C Edenius, U Grabowska
    J Transl Med, 2018-05-09;16(1):125.
    Applications: Bioassay
  2. Degraded melanocores are incompetent to protect epidermal keratinocytes against UV damage
    Authors: WJ Yi, MY Su, Y Shi, S Jiang, SZ Xu, TC Lei
    Cell Cycle, 2018-04-25;0(0):1-40.
    Species: Human
    Sample Types: Melanocores
    Applications: Bioassay
  3. Suppression and resolution of autoimmune arthritis by rhesus ?-defensin-1, an immunomodulatory macrocyclic peptide
    Authors: JB Schaal, DQ Tran, A Subramania, R Patel, T Laragione, KD Roberts, K Trinh, P Tongaonkar, PA Tran, D Minond, GB Fields, P Beringer, AJ Ouellette, PS Gulko, ME Selsted
    PLoS ONE, 2017-11-16;12(11):e0187868.
    Applications: Bioassay
  4. Cathepsin K-mediated Notch1 activation contributes to neovascularization in response to hypoxia.
    Authors: Jiang H, Cheng X, Shi G, Hu L, Inoue A, Yamamura Y, Wu H, Takeshita K, Li X, Huang Z, Song H, Asai M, Hao C, Unno K, Koike T, Oshida Y, Okumura K, Murohara T, Kuzuya M
    Nat Commun, 2014-06-04;5(0):3838.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Bioassay
  5. Regulation of cathepsin g reduces the activation of proinsulin-reactive T cells from type 1 diabetes patients.
    Authors: Zou F, Schafer N, Palesch D, Brucken R, Beck A, Sienczyk M, Kalbacher H, Sun Z, Boehm BO, Burster T
    PLoS ONE, 2011-08-05;6(8):e22815.
    Species: Human
    Sample Types: Protein
    Applications: Enzyme Assay
  6. Generation of endostatin by matrix metalloproteinase and cathepsin from human limbocorneal epithelial cells cultivated on amniotic membrane.
    Authors: Ma DH, Yao JY, Kuo MT, See LC, Lin KY, Chen SC, Chen JK, Chao AS, Wang SF, Lin KK
    Invest. Ophthalmol. Vis. Sci., 2007-02-01;48(2):644-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Cystatin M/E is a high affinity inhibitor of cathepsin V and cathepsin L by a reactive site that is distinct from the legumain-binding site. A novel clue for the role of cystatin M/E in epidermal cornification.
    Authors: Cheng T, Hitomi K, van Vlijmen-Willems IM, de Jongh GJ, Yamamoto K, Nishi K, Watts C, Reinheckel T, Schalkwijk J, Zeeuwen PL
    J. Biol. Chem., 2006-03-24;281(23):15893-9.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay

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Recombinant Human Cathepsin V Protein, CF
By Anonymous on 04/21/2022

Reason for Rating: It works well to find the Cathepsin V inhibitors.
Recombinant Human Cathepsin V Protein, CF 1080-CY