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Recombinant Mouse Netrin-4 Protein

Catalog #: 1132-N4
4 Citations Datasheet / COA / SDS

Discontinued Product

1132-N4 has been discontinued.
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Recombinant Mouse Netrin-4 Protein Summary

Product Specifications

Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
Activity

Measured by its ability to bind rrUNC5H2/Fc Chimera in a functional ELISA.

Immobilized rrUNC5H2/Fc Chimera at 5 µg/mL (100 µL/well) can bind rmNetrin-4 with a linear range of 6-400 ng/mL.

Source
Mouse myeloma cell line, NS0-derived mouse Netrin-4 protein
Leu19-Val628 & Ala26-Val628, both with a C-terminal 10-His tag
Accession #
Q9JI33
N-terminal Sequence
Analysis
Leu19 & Ala26
Predicted Molecular Mass
69 kDa
SDS-PAGE
85 kDa, reducing conditions

Product Datasheets

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1132-N4 (with carrier)

Product Datasheet
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1132-N4/CF (carrier free)

Discontinued Product

1132-N4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Netrin-4

Netrins/UNC-6 (netr: Sanskrit for “one who guides”) are a family of laminin-related small proteins that are involved in neurite outgrowth and axon guidance. Netrins binds to the DCC and UNC5 family of receptors to attract or repel axons. Mouse Netrin-4, also known as beta -Netrin, is synthesized as a 629 amino acid (aa) precursor that contains a 19 aa signal sequence, a 428 aalaminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, and a 182 aa C domain rich in basic aa residues that serves as a heparin binding site. Netrin-4 has been reported to exist as both a monomer and a dimer. The dimeric form was reported to be the less active of the two. Mouse Netrin-4 shares 31%, 29% and 25% aa sequence identity with mouse Netrin-1, -3, and -G1a, respectively. It also shares 89% aa sequence identity with human Netrin-4. Netrin-4 has widespread expression, occurring in nervous tissues such as embryonic floor plate and postnatal neurons such as cerebellar granule cells and hippocampal pyramidal cells. It also is found in non-neural tissues such as adult Bowman’s capsule and medullary tubular epithelium in kidney, and embryonic pancreatic and intestinal epithelium, plus cells of the ureteric bud. Netrin-4 expression is often associated with basement membrane. Netrin-4 has been shown to initiate axon outgrowth from olfactory bulb explants (1 - 7).

References
  1. McFarlane, S. (2000) Biochem. Cell. Biol. 78:563.
  2. Kennedy, T.E. (2000) Biochem. Cell Biol. 78:569.
  3. Yu, T.W. and C.I. Bargmann (2001) Nature Neurosci. 4(Suppl):1169.
  4. Stein, E. et al. (2001) Science 291:1976.
  5. Koch, M. et al. (2000) J. Cell Biol. 151:221.
  6. Yin, Y. et al. (2000) Mech. Dev. 96:115.
  7. Livesey, F.J. (1999) Cell. Mol. Life Sci. 56:62.
Entrez Gene IDs
59277 (Human); 57764 (Mouse)
Alternate Names
beta-Netrin; hepar-derived netrin-like protein; netrin 4; Netrin4; Netrin-4; NTN4; PRO3091

Product Specific Notices

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,565,331, 6,096,866, 6,017,714, 6,309,638, 6,670,451, and other U.S. and international patents pending.

Citations for Recombinant Mouse Netrin-4 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Dorsal horn interneuron-derived Netrin-4 contributes to spinal sensitization in chronic pain via Unc5B
    Authors: Toshihide Yamashita
    J. Exp. Med., 2016-11-17;0(0):.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  2. Therapeutic effects of topical netrin-4 inhibits corneal neovascularization in alkali-burn rats.
    Authors: Han, Yun, Shao, Yi, Liu, Tingting, Qu, Yang-Luo, Li, Wei, Liu, Zuguo
    PLoS ONE, 2015-04-08;10(4):e0122951.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  3. Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia.
    Authors: Hoang S, Liauw J, Choi M
    J. Cereb. Blood Flow Metab., 2008-11-05;29(2):385-97.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Netrin-1 and kidney injury. I. Netrin-1 protects against ischemia-reperfusion injury of the kidney.
    Authors: Wang W, Reeves WB, Ramesh G
    Am. J. Physiol. Renal Physiol., 2008-01-23;294(4):F739-47.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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