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Recombinant Human Fc gamma RI/CD64 Protein, CF

Catalog #: 1257-FC
24 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1257-FC-050
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Recombinant Human Fc gamma RI/CD64 Protein Binding Activity
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Citations (24)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Fc gamma RI/CD64 Protein, CF Summary

Why choose R&D Systems Fc gamma RI Protein?

  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95%.
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

Other species of Fc gamma RI are available here Fc gamma RI Proteins

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind human IgG with an estimated Kd <0.15 nM.
Source
Mouse myeloma cell line, NS0-derived human Fc gamma RI/CD64 protein
Gln16-Pro288, with a C-terminal 6-His tag
Accession #
P12314
N-terminal Sequence
Analysis
No results obtained: Gln16 predicted
Predicted Molecular Mass
31.4 kDa
SDS-PAGE
45-55 kDa, reducing conditions

Product Datasheets

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1257-FC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1257-FC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human Fc gamma RI/CD64 Protein Binding Activity View Larger

When Human IgG is coated at 0.5 µg/mL, Recombinant Human Fc gamma RI/CD64 binds with an estimated Kd<0.15 nM.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RI/CD64

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1-3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors
(~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Three highly homologous genes (A, B, and C) sharing 98% identity at the nucleotide level have been identified for the human CD64 group (1). Fc gamma  RI is transmembrane protein with three extracellular Ig-like domains, and it delivers an activating signal via the associated Fc R gamma accessory chain. The genes for Fc gamma  RIB and Fc gamma  RIC contain stop codons within their membrane proximal Ig-like domains indicating possible secreted receptors (1, 6). An mRNA splice variant of Fc gamma RIB has a deletion of the membrane-proximal Ig-like domain and encodes a putative transmembrane receptor (6). The high affinity recognition of IgG by Fc gamma  RI permits the triggering of effector responses at low IgG concentrations typical of early immune responses (2). Fc gamma  RI is expressed constitutively on monocytes and macrophages and can be induced on neutrophils and eosinophils (1, 4). Its expression is up-regulated during bacterial infections and sepsis.

References
  1. Van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.
  6. Ernst, L. et al. (1998) Mol Immunol. 35:943.
Long Name
Fc gamma Receptor I
Entrez Gene IDs
2209 (Human); 14129 (Mouse); 295279 (Rat); 102147198 (Cynomolgus Monkey)
Alternate Names
CD64 antigen; CD64; CD64a; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc gamma RI; FCG1; Fc-gamma receptor I B2; Fc-gamma RI; Fc-gamma RIA; FcgammaRIa; FCGR1; FcgRI; FcgRIA; FCRI; FcRIA; FLJ18345; high affinity Ia, receptor for (CD64); high affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I

Citations for Recombinant Human Fc gamma RI/CD64 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Repurposing Fc gamma receptor I (Fc?RI, CD64) for site-oriented monoclonal antibody capture: A proof-of-concept study for real-time detection of tumor necrosis factor-alpha (TNF -?)
    Authors: Çapk?n, E;Kutlu, A;Yüce, M;
    Heliyon
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Optimized ACE2 decoys neutralize antibody-resistant SARS-CoV-2 variants through functional receptor mimicry and treat infection in vivo
    Authors: JA Torchia, AH Tavares, LS Carstensen, DY Chen, J Huang, T Xiao, S Mukherjee, PM Reeves, H Tu, AE Sluder, B Chen, DN Kotton, RA Bowen, M Saeed, MC Poznansky, GJ Freeman
    Science Advances, 2022-12-07;8(49):eabq6527.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  3. Fc-gamma receptor-mediated crosslinking co-defines the immunostimulatory activity of anti-human CD96 antibodies
    Authors: A Rogel, FM Ibrahim, SM Thirdborou, F Renart-Dep, CN Birts, SL Buchan, X Preville, EV King, A Al-Shamkha
    JCI Insight, 2022-10-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Combined IgE neutralization and Bifidobacterium longum supplementation reduces the allergic response in models of food allergy
    Authors: SB An, BG Yang, G Jang, DY Kim, J Kim, SM Oh, N Oh, S Lee, JY Moon, JA Kim, JH Kim, YJ Song, HW Hyun, J Kim, K Lee, D Lee, MJ Kwak, BK Kim, YK Park, CP Hong, JH Kim, HS Lim, MS Ryu, HT Jin, SW Lee, YS Chang, HS Park, YC Sung, MH Jang
    Nature Communications, 2022-09-27;13(1):5669.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  5. Discovery of a novel Igbeta and FcgammaRIIB cross-linking antibody, ASP2713, and its potential application in the treatment of systemic lupus erythematosus
    Authors: Y Kitanaga, D Yamajuku, S Kubo, K Nakamura, M Maeda, M Seki, Y Kaneko, F Kinugasa, T Morokata, Y Kondo, H Yoshinari, S Nakayamada, T Sumida, Y Tanaka
    International immunopharmacology, 2021-11-12;101(0):108343.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture, Surface Plasmon Resonance (SPR
  6. Human Monoclonal Antibodies against NS1 Protein Protect against Lethal West Nile Virus Infection
    Authors: AW Wessel, MP Doyle, TB Engdahl, J Rodriguez, JE Crowe, MS Diamond
    MBio, 2021-10-12;12(5):e0244021.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  7. Developing a multivariate prediction model of antibody features associated with protection of malaria-infected pregnant women from placental malaria
    Authors: EH Aitken, T Damelang, A Ortega-Paj, A Alemu, W Hasang, S Dini, HW Unger, M Ome-Kaius, MA Nielsen, A Salanti, J Smith, S Kent, PM Hogarth, BD Wines, JA Simpson, A Chung, SJ Rogerson
    Elife, 2021-06-29;10(0):.
    Species: Human
    Sample Types: Plasma
    Applications: Bioassay
  8. High neutralizing potency of swine glyco-humanized polyclonal antibodies against SARS-CoV-2
    Authors: B Vanhove, O Duvaux, J Rousse, PJ Royer, G Evanno, C Ciron, E Lheriteau, L Vacher, N Gervois, R Oger, Y Jacques, S Conchon, A Salama, R Duchi, I Lagutina, A Perota, P Delahaut, M Ledure, M Paulus, RT So, CK Mok, R Bruzzone, M Bouillet, S Brouard, E Cozzi, C Galli, D Blanchard, JM Bach, JP Soulillou
    European Journal of Immunology, 2021-03-02;0(0):.
    Species: Porcine
    Sample Types: Protein
    Applications: Bioassay
  9. An IgG-based bispecific antibody for improved dual targeting in PSMA-positive cancer
    Authors: L Zekri, F Vogt, L Osburg, S Müller, J Kauer, T Manz, M Pflügler, A Maurer, JS Heitmann, I Hagelstein, M Märklin, S Hörner, T Todenhöfer, C Calaminus, A Stenzl, B Pichler, C la Fougère, MA Schneider, HG Rammensee, L Zender, B Sipos, HR Salih, G Jung
    Embo Molecular Medicine, 2020-12-29;0(0):e11902.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  10. Antibodies targeting epitopes on the cell-surface form of NS1 protect against Zika virus infection during pregnancy
    Authors: AW Wessel, N Kose, RG Bombardi, V Roy, W Chantima, J Mongkolsap, MA Edeling, CA Nelson, I Bosch, G Alter, GR Screaton, DH Fremont, JE Crowe, MS Diamond
    Nat Commun, 2020-10-19;11(1):5278.
    Species: Human
    Sample Types: Antibody
    Applications: ELISA Capture
  11. Insertion of N-Terminal Hinge Glycosylation Enhances Interactions of the Fc Region of Human IgG1 Monomers with Glycan-Dependent Receptors and Blocks Hemagglutination by the Influenza Virus
    Authors: PA Blundell, D Lu, M Wilkinson, A Dell, S Haslam, RJ Pleass
    J. Immunol., 2019-01-25;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA (Capture), Surface Plasmon Resonance
  12. Fc engineering of anti-Nectin-2 antibody improved thrombocytopenic adverse event in monkey
    Authors: T Oshima, H Miyashita, Y Ishimura, Y Ito, Y Tanaka, A Hori, T Kokubo, T Kurokawa
    PLoS ONE, 2018-05-03;13(5):e0196422.
    Applications: Bioassay
  13. Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies
    Authors: F Arce Varga, AJS Furness, K Litchfield, K Joshi, R Rosenthal, E Ghorani, I Solomon, MH Lesko, N Ruef, C Roddie, JY Henry, L Spain, A Ben Aissa, A Georgiou, YNS Wong, M Smith, D Strauss, A Hayes, D Nicol, T O'Brien, L Mårtensson, A Ljungars, I Teige, B Frendéus, M Pule, T Marafioti, M Gore, J Larkin, S Turajlic, C Swanton, KS Peggs, SA Quezada
    Cancer Cell, 2018-03-22;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  14. Rapid screening of IgG quality attributes - effects on Fc receptor binding
    Authors: KPM Geuijen, C Oppers-Tie, DF Egging, PJ Simons, L Boon, RBM Schasfoort, MHM Eppink
    FEBS Open Bio, 2017-09-05;7(10):1557-1574.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  15. Enzymatic inactivation of endogenous IgG by IdeS enhances�therapeutic antibody efficacy
    Authors: S Järnum, A Runström, R Bockermann, L Winstedt, M Crispin, C Kjellman
    Mol. Cancer Ther., 2017-05-22;0(0):.
    Species: Human
    Sample Types: Antibody
    Applications: Bioassay
  16. Novel drug and soluble target tolerant antidrug antibody assay for therapeutic antibodies bearing the P329G mutation
    Authors: U Wessels, E Schick, M Ritter, F Kowalewsky, J Heinrich, K Stubenrauc
    Bioanalysis, 2017-05-18;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  17. Anti-CD20 Antibody with Multimerized Fc Domains: A Novel Strategy To Deplete B Cells and Augment Treatment of Autoimmune Disease.
    Authors: Zhang X, Olsen H, Chen S, So E, Zhou H, Burch E, Merigeon E, Block D, Strome S
    J Immunol, 2015-12-22;196(3):1165-76.
    Applications: Surface Plasmon Resonance
  18. Chemically synthesized molecules with the targeting and effector functions of antibodies.
    Authors: McEnaney P, Fitzgerald K, Zhang A, Douglass E, Shan W, Balog A, Kolesnikova M, Spiegel D
    J Am Chem Soc, 2014-12-16;136(52):18034-43.
    Applications: Binding Assay
  19. Mechanisms of protection against Clostridium difficile infection by the monoclonal antitoxin antibodies actoxumab and bezlotoxumab.
    Authors: Yang Z, Ramsey J, Hamza T, Zhang Y, Li S, Yfantis H, Lee D, Hernandez L, Seghezzi W, Furneisen J, Davis N, Therien A, Feng H
    Infect Immun, 2014-12-08;83(2):822-31.
    Species: Human
    Sample Types: Antibody
    Applications: Bioassay
  20. GlycoDelete engineering of mammalian cells simplifies N-glycosylation of recombinant proteins.
    Authors: Meuris L, Santens F, Elson G, Festjens N, Boone M, Dos Santos A, Devos S, Rousseau F, Plets E, Houthuys E, Malinge P, Magistrelli G, Cons L, Chatel L, Devreese B, Callewaert N
    Nat Biotechnol, 2014-04-20;32(5):485-9.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  21. Engineering of a novel anti-CD40L domain antibody for treatment of autoimmune diseases.
    Authors: Xie J, Yamniuk A, Borowski V, Kuhn R, Susulic V, Rex-Rabe S, Yang X, Zhou X, Zhang Y, Gillooly K, Brosius R, Ravishankar R, Waggie K, Mink K, Price L, Rehfuss R, Tamura J, An Y, Cheng L, Abramczyk B, Ignatovich O, Drew P, Grant S, Bryson J, Suchard S, Salter-Cid L, Nadler S, Suri A
    J Immunol, 2014-03-26;192(9):4083-92.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  22. Increasing FcgammaRIIa affinity of an FcgammaRIII-optimized anti-EGFR antibody restores neutrophil-mediated cytotoxicity.
    Authors: Derer S, Glorius P, Schlaeth M, Lohse S, Klausz K, Muchhal U, Desjarlais J, Humpe A, Valerius T, Peipp M
    MAbs, 2013-12-11;6(2):409-21.
    Applications: Surface Plasmon Resonance
  23. Identification of a high affinity FcgammaRIIA-binding peptide that distinguishes FcgammaRIIA from FcgammaRIIB and exploits FcgammaRIIA-mediated phagocytosis and degradation.
    Authors: Berntzen G, Andersen JT, Ustgard K, Michaelsen TE, Mousavi SA, Qian JD, Kristiansen PE, Lauvrak V, Sandlie I
    J. Biol. Chem., 2008-10-28;284(2):1126-35.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  24. Optimization of antibody binding to FcgammaRIIa enhances macrophage phagocytosis of tumor cells.
    Authors: Richards JO, Karki S, Lazar GA, Chen H, Dang W, Desjarlais JR
    Mol. Cancer Ther., 2008-08-01;7(8):2517-27.
    Species: Human
    Sample Types: Antibody
    Applications: Surface Plasmon Resonance

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Recombinant Human Fc gamma RI/CD64 Protein, CF
By Anonymous on 11/07/2017
Application: Immunoassay Standard