Recombinant Human Fas Ligand/TNFSF6 Protein Summary
Product Specifications
Pro134-Leu281, with an N-terminal Met and 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
126-FL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
126-FL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Fas Ligand/TNFSF6
Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a 40 kDa type II transmembrane member of the TNF superfamily of proteins. Its ability to induce apoptosis in target cells plays an important role in the development, homeostasis, and function of the immune system (1). Mature human Fas Ligand consists of a 179 amino acid (aa) extracellular domain (ECD), a 22 aa transmembrane segment, and a 80 aa cytoplasmic domain (2). Within the ECD, human Fas Ligand shares 81% and 78% aa sequence identity with mouse and rat Fas Ligand, respectively. Both mouse and human Fas Ligand are active on mouse and human cells (2, 3). Fas Ligand is expressed on the cell surface as a nondisulfide-linked homotrimer on activated CD4+ Th1 cells, CD8+ cytotoxic T cells, and NK cells (1). Fas Ligand binding to Fas/CD95 on an adjacent cell triggers apoptosis in the Fas‑expressing cell (2, 4). Fas Ligand also binds DcR3 which is a soluble decoy receptor that interferes with Fas Ligand-induced apoptosis (5). Fas Ligand can be released from the cell surface by metalloproteinases as a 26 kDa soluble molecule which remains trimeric (6, 7). Shed Fas Ligand retains the ability to bind Fas, although its ability to trigger apoptosis is dramatically reduced (6, 7). In the absence of TGF‑ beta, however, Fas Ligand/Fas interactions instead promote neutrophil-mediated inflammatory responses (3, 8). Fas Ligand itself transmits reverse signals that costimulate the proliferation of freshly antigen-stimulated T cells (9). Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintance of immune privileged sites (10). This function is exploited by tumor cells which evade immune surveillance by upregulating Fas Ligand to kill tumor infiltrating lymphocytes (8, 11). In gld mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity (12, 13).
- Lettau, M. et al. (2008) Curr. Med. Chem. 15:1684.
- Takahashi, T. et al. (1994) Int. Immunol. 6:1567.
- Seino, K-I. et al. (1998) J. Immunol. 161:4484.
- Suda, T. et al. (1993) Cell 75:1169.
- Pitti, R.M. et al. (1998) Nature 396:699.
- Schneider, P. et al. (1998) J. Exp. Med. 187:1205.
- Tanaka, M. et al. (1998) Nature Med. 4:31.
- Chen, J.-J. et al. (1998) Science 282:1714.
- Suzuki, I. and P.J. Fink (2000) Proc. Natl. Acad. Sci. 97:1707.
- Ferguson, T.A. and T.S. Griffith (2006) Immunol. Rev. 213:228.
- Ryan, A.E. et al. (2005) Cancer Res. 65:9817.
- Takahashi, T. et al. (1994) Cell 76:969.
- Lynch, D.H. et al. (1994) Immunity 1:131.
Citations for Recombinant Human Fas Ligand/TNFSF6 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Fructose-1,6-bisphosphatase 1 dephosphorylates IkappaBalpha and suppresses colorectal tumorigenesis
Authors: W Zhu, H Chu, Y Zhang, T Luo, H Yu, H Zhu, Y Liu, H Gao, Y Zhao, Q Li, X Wang, G Li, W Yang
Cell Research, 2023-01-16;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Targeting hypoxia-induced tumor stemness by activating pathogen-induced stem cell niche defense.
Authors: Bhuyan S, Pal B, Pathak L, Saikia P, Mitra S, Gayan S, Mokhtari R, Li H, Ramana C, Baishya D, Das B
Front Immunol, 2022-09-29;13(0):933329.
Species: Complex Species Category
Sample Types: Whole Cells
Applications: Bioassay -
Adrenomedullin and truncated peptide adrenomedullin(22-52) affect chondrocyte response to apoptotis in vitro: downregulation of FAS protects chondrocyte from cell death
Authors: F Velard, A Chatron-Co, D Côme, MD Ah-Kioon, H Lin, N Hafsia, M Cohen-Sola, HK Ea, F Lioté
Sci Rep, 2020-10-07;10(1):16740.
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay -
An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease
Authors: AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle
MAbs, 2018-03-05;0(0):1-43.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR -
TLR3 Ligand Poly(I:C) Exerts Distinct Actions in Synovial Fibroblasts When Delivered by Extracellular Vesicles
Authors: M Frank-Bert, DS Pisetsky, C Kolling, BA Michel, RE Gay, A Jüngel, S Gay
Front Immunol, 2018-01-29;9(0):28.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
HAT1 induces lung cancer cell apoptosis via up regulating Fas
Authors: N Han, L Shi, Q Guo, W Sun, Y Yu, L Yang, X Zhang, M Zhang
Oncotarget, 2017-09-23;8(52):89970-89977.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Suberoylanilide hydroxamic acid increases anti-cancer effect of tumor necrosis factor-? through up-regulation of TNF receptor 1 in lung cancer cells.
Authors: Bo Ra You, Bo Ram Han, Woo Hyun Park
Oncotarget, 2017-03-14;0(0):1949-2553.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Endotoxemia contributes to CD27+ memory B-cell apoptosis via enhanced sensitivity to Fas ligation in patients with Cirrhosis
Sci Rep, 2016-11-18;6(0):36862.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Cytoplasmic myosin-exposed apoptotic cells appear with caspase-3 activation and enhance CLL cell viability.
Authors: Cui X, Zhang L, Magli A, Catera R, Yan X, Griffin D, Rothstein T, Barrientos J, Kolitz J, Allen S, Rai K, Chiorazzi N, Chu C
Leukemia, 2015-07-29;30(1):74-85.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Decoy Receptor 3 Improves Survival in Experimental Sepsis by Suppressing the Inflammatory Response and Lymphocyte Apoptosis.
Authors: Liang D, Hou Y, Lou X, Chen H
PLoS ONE, 2015-06-29;10(6):e0131680.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Targeted 25-hydroxyvitamin D3 1alpha-hydroxylase adoptive gene therapy ameliorates dss-induced colitis without causing hypercalcemia in mice.
Authors: Li B, Baylink D, Walter M, Lau K, Meng X, Wang J, Cherkas A, Tang X, Qin X
Mol Ther, 2014-10-20;23(2):339-51.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Suberoylanilide hydroxamic acid-induced HeLa cell death is closely correlated with oxidative stress and thioredoxin 1 levels.
Authors: You B, Park W
Int J Oncol, 2014-03-12;44(5):1745-55.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
In vivo administration of a JAK3 inhibitor during acute SIV infection leads to significant increases in viral load during chronic infection.
Authors: Takahashi Y, Byrareddy S, Albrecht C, Brameier M, Walter L, Mayne A, Dunbar P, Russo R, Little D, Villinger T, Khowawisetsut L, Pattanapanyasat K, Villinger F, Ansari A
PLoS Pathog, 2014-03-06;10(3):e1003929.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Bioassay -
Cross-species withdrawal of MCL1 facilitates postpartum uterine involution in both the mouse and baboon.
Authors: Kyathanahalli, Chandras, Marks, Jason, Nye, Kennedy, Lao, Belinda, Albrecht, Eugene D, Aberdeen, Graham W, Nathanielsz, Peter W, Jeyasuria, Panchara, Condon, Jennifer
Endocrinology, 2013-10-18;154(12):4873-84.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Granule exocytosis mediates immune surveillance of senescent cells.
Authors: Sagiv, A, Biran, A, Yon, M, Simon, J, Lowe, S W, Krizhanovsky, V
Oncogene, 2012-07-02;32(15):1971-7.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Antiapoptotic signaling via MCL1 confers resistance to caspase-3-mediated apoptotic cell death in the pregnant human uterine myocyte.
Authors: Stephenson-Famy A, Marks J, Suresh A
Mol. Endocrinol., 2011-12-22;26(2):320-30.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Murine insulin growth factor-like (IGFL) and human IGFL1 proteins are induced in inflammatory skin conditions and bind to a novel tumor necrosis factor receptor family member, IGFLR1.
Authors: Lobito AA, Ramani SR, Tom I, Bazan JF, Luis E, Fairbrother WJ, Ouyang W, Gonzalez LC
J. Biol. Chem., 2011-03-31;286(21):18969-81.
Species: Human
Sample Types:
Applications: Surface Plasmon Resonance -
Acute depletion of activated memory B cells involves the PD-1 pathway in rapidly progressing SIV-infected macaques.
Authors: Titanji K, Velu V, Chennareddi L
J. Clin. Invest., 2010-10-25;120(11):3878-90.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Bioassay -
Proton pump inhibitors induce apoptosis of human B-cell tumors through a caspase-independent mechanism involving reactive oxygen species.
Authors: De Milito A, Iessi E, Logozzi M, Lozupone F, Spada M, Marino ML, Federici C, Perdicchio M, Matarrese P, Lugini L, Nilsson A, Fais S
Cancer Res., 2007-06-01;67(11):5408-17.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?
The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.
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Is Recombinant Human Fas Ligand/TNFSF6 Protein (Catalog # 126-FL) a trimer in solution?
In house, we observed this 126-FL protein mostly in monomeric form with some dimers/trimers present while in solution. This protein can be oligamerized in the presence of the cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
Reviews for Recombinant Human Fas Ligand/TNFSF6 Protein
Average Rating: 3 (Based on 1 Review)
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Reason for Rating: The website states that the purity of the protein is >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. What we see is much less than this. At least the protein is functional.
We used the ligand for a pulldown assay and unfortunately one of the major contaminating protein is the same size as our protein, so we can not analyse our experiment. It was an expensive experiment to run for a small amount of supposedly pure ligand.
