Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein, CF

Catalog #: 1557-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1557-CD-050

Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein Bioactivity

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All CD320/TCblR/8D6A Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD320/TCblR/8D6A Fc Chimera is immobilized at 25 ng/mL (100 μL/well), the concentration of Recombinant Human Transcobalamin II (Catalog # 7895-TC) that produces 50% of the optimal binding response is 0.5-3 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human CD320/TCblR/8D6A protein

Human CD320 (Leu31-Val231) Accession # Q9NPF0	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q9NPF0

N-terminal Sequence
Analysis

Leu31 and Ser36

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

47.2 kDa (monomer)

SDS-PAGE

80-90 kDa, reducing conditions

Product Datasheets

1557-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1557-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Human CD320/TCblR/8D6A Fc Chimera (Catalog # 1557-CD) is coated at 25 ng/mL, Recombinant Human Transcobalamin II (Catalog # 7895-TC) binds with an ED₅₀ of 0.5-3 ng/mL.

Reconstitution Calculator

Background: CD320/TCblR/8D6A

CD320, also known as the Transcobalamin II receptor (TCb1R) and 8D6A, is an approximately 60 kDa transmembrane member of the LDL receptor family (1). Mature human CD320 consists of a 199 amino acid (aa) extracellular domain with two cysteine-rich LDLR-A repeats, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2). Within the extracellular domain, human CD320 shares 52% aa sequence identity with mouse and rat CD320. CD320 is a heavily glycosylated protein that is expressed as a noncovalent dimer in the kidney, placenta, liver, intestine, and secondary lymphoid organs (3 - 5). It functions as an endocytic receptor for circulating complexes of Cobalamin (Vitamin B₁₂) and Transcobalamin II (3). In the immune system, CD320 is expressed by germinal center B cells and follicular dendritic cells (FDC) and provides a co‑stimulatory signal to B cells during the germinal center reaction (2, 6 - 8). CD320 also cooperates with CD44 in promoting the development of B cell lymphomas (9).

References