Home / BAFF/BLyS/TNFSF13B / Recombinant Mouse BAFF/BLyS/TNFSF13B Protein

Recombinant Mouse BAFF/BLyS/TNFSF13B Protein

Catalog #: 2106-BF
21 Citations Datasheet / COA / SDS
Newer Version Available: 8876-BF
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His-tagged

Discontinued Product

2106-BF has been discontinued and is replaced by 8876-BF.

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Citations (21)
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Recombinant Mouse BAFF/BLyS/TNFSF13B Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.1‑0.5 ng/mL in the presence of goat anti‑mouse IgM µ chain.
Source
Mouse myeloma cell line, NS0-derived mouse BAFF/BLyS/TNFSF13B protein
MHHHHHHHHHH GGGSGGGSGGGS IEGR Mouse BAFF
(Ala127-Leu309)
Accession # Q9WU72
N-terminus C-terminus
Accession #
Q9WU72
N-terminal Sequence
Analysis
Met and Ala127
Predicted Molecular Mass
23.2 kDa
SDS-PAGE
24 kDa, reducing conditions

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2106-BF (with carrier)

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2106-BF/CF (carrier free)

Discontinued Product

2106-BF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: BAFF/BLyS/TNFSF13B

B-cell activating factor (BAFF), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a 32 kDa transmembrane glycoprotein in the TNF ligand superfamily. It is involved in multiple aspects of immune system regulation, particularly towards B cells (1, 2). Mature mouse BAFF consists of a 47 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 241 aa extracellular domain (ECD) with a stalk region and one TNF-like domain (3, 4). Within aa 127-309 of the ECD, mouse BAFF shares 72% aa sequence identity with human BAFF. It can be expressed as a homotrimer or as a heteromer in association with the related TNFSF member APRIL (4, 5). A 18 kDa fragment containing the TNF-like domain can be released by proteolysis between Arg126 and Ala127 (4). Soluble BAFF is stored intracellularly in neutrophils and released upon inflammatory stimulation (6). Alternative splicing generates an isoform termed deltaBAFF that lacks 19 aa between the proteolytic cleavage site and the TNF-like domain. deltaBAFF can form heteromers with BAFF and negatively regulates BAFF function (7). BAFF is produced by many hematopoietic cell types including by monocytes, macrophages, neutrophils, dendritic cells, and T cells and also by adipocytes (1, 2, 8). Both BAFF and APRIL are functional ligands for the TNF receptor superfamily members BCMA and TACI, and BAFF additionally binds and signals through BAFF R (9, 10). All three receptors are primarily expressed by B cells (10). BAFF plays a critical role in the development and survival of B lineage cells (2, 11, 12). Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity (13). Soluble BAFF is elevated in B cell malignancies, autoimmunity, and other immune disorders (1). In addition, BAFF co-stimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells (14-16). BAFF also promotes monocyte survival, proinflammatory cytokine secretion, and differentiation to macrophages (17).

References
  1. Lied, G.A. and A. Berstad (2011) Scand. J. Immunol. 73:1.
  2. Mackay, F. et al. (2010) Immunol. Rev. 237:205.
  3. Moore, P.A. et al. (1999) Science 285:260.
  4. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
  5. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  6. Scapini, P. et al. (2003) J. Exp. Med. 197:297.
  7. Gavin, A.L. et al. (2003) J. Biol. Chem. 278:38220.
  8. Alexaki, V.-I. et al. (2009) J. Immunol. 183:5948.
  9. Yu, G. et al. (2000) Nat. Immunol. 1:252.
  10. Thompson, J.S. et al. (2001) Science 293:2108.
  11. Schiemann, B. et al. (2001) Science 293:2111.
  12. Litinskiy, M.B. et al. (2002) Nat. Immunol. 3:822.
  13. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
  14. Huard, B. et al. (2001) J. Immunol. 167:6225.
  15. Sutherland, A.P.R. et al. (2005) J. Immunol. 174:5537.
  16. Walters, S. et al. (2009) J. Immunol. 182:793.
  17. Chang, S.K. et al. (2006) Blood 108:2687.
Long Name
B cell Activating Factor
Entrez Gene IDs
10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)
Alternate Names
ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4

Citations for Recombinant Mouse BAFF/BLyS/TNFSF13B Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Cyclin D3 Governs Clonal Expansion of Dark Zone Germinal Center B Cells
    Authors: P Ramezani-R, C Chen, Z Zhu, RC Rickert
    Cell Rep, 2020-11-17;33(7):108403.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Limitations of Qdot labelling compared to directly-conjugated probes for single particle tracking of B cell receptor mobility
    Authors: L Abraham, HY Lu, RC Falcão, J Scurll, T Jou, B Irwin, R Tafteh, MR Gold, D Coombs
    Sci Rep, 2017-09-12;7(1):11379.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Interactions between fibroblastic reticular cells and B cells promote mesenteric lymph node lymphangiogenesis
    Authors: L Kumar Dube, P Karempudi, SA Luther, B Ludewig, NL Harris
    Nat Commun, 2017-08-28;8(1):367.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Intrinsic transcriptional heterogeneity in B cells controls early class switching to IgE
    Authors: Yee Ling Wu
    J. Exp. Med, 2016-12-19;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. MRL Strains Have a BAFFR Mutation without Functional Consequence
    PLoS ONE, 2016-05-05;11(5):e0154518.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. mTOR-Dependent and Independent Survival Signaling by PI3K in B Lymphocytes.
    Authors: Kaileh M, Vazquez E, MacFarlane A, Campbell K, Kurosaki T, Siebenlist U, Sen R
    PLoS ONE, 2016-01-19;11(1):e0146955.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Immunosuppressive and Prometastatic Functions of Myeloid-Derived Suppressive Cells Rely upon Education from Tumor-Associated B Cells.
    Authors: Bodogai M, Moritoh K, Lee-Chang C, Hollander C, Sherman-Baust C, Wersto R, Araki Y, Miyoshi I, Yang L, Trinchieri G, Biragyn A
    Cancer Res, 2015-07-16;75(17):3456-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
    Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
    Leukemia, 2015-07-03;30(1):163-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Combined loss of the BH3-only proteins Bim and Bmf restores B-cell development and function in TACI-Ig transgenic mice.
    Authors: Woess C, Tuzlak S, Labi V, Drach M, Bertele D, Schneider P, Villunger A
    Cell Death Differ, 2015-02-20;22(9):1477-88.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. The effects of Jieduquyuzishen prescription-treated rat serum on the BAFF/BAFF-R signal pathway.
    Authors: Wu D, Xu L, Wen C, Xie G, Ji J, Pan J, Jiao Y, Fan Y
    PLoS ONE, 2015-02-17;10(2):e0118462.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Toll-like receptor ligands sensitize B-cell receptor signalling by reducing actin-dependent spatial confinement of the receptor.
    Authors: Freeman S, Jaumouille V, Choi K, Hsu B, Wong H, Abraham L, Graves M, Coombs D, Roskelley C, Das R, Grinstein S, Gold M
    Nat Commun, 2015-02-03;6(0):6168.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. The immunoregulator soluble TACI is released by ADAM10 and reflects B cell activation in autoimmunity.
    Authors: Hoffmann F, Kuhn P, Laurent S, Hauck S, Berer K, Wendlinger S, Krumbholz M, Khademi M, Olsson T, Dreyling M, Pfister H, Alexander T, Hiepe F, Kumpfel T, Crawford H, Wekerle H, Hohlfeld R, Lichtenthaler S, Meinl E
    J Immunol, 2014-12-10;194(2):542-52.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Uracil excision by endogenous SMUG1 glycosylase promotes efficient Ig class switching and impacts on A:T substitutions during somatic mutation.
    Authors: Dingler F, Kemmerich K, Neuberger M, Rada C
    Eur J Immunol, 2014-05-27;44(7):1925-35.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. The protein tyrosine phosphatase PTP1B is a negative regulator of CD40 and BAFF-R signaling and controls B cell autoimmunity.
    Authors: Medgyesi, David, Hobeika, Elias, Biesen, Robert, Kollert, Florian, Taddeo, Adriano, Voll, Reinhard, Hiepe, Falk, Reth, Michael
    J Exp Med, 2014-03-03;211(3):427-40.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Rgs13 constrains early B cell responses and limits germinal center sizes.
    Authors: Hwang I, Hwang K, Park C, Harrison K, Kehrl J
    PLoS ONE, 2013-03-22;8(3):e60139.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. TNFR-Associated Factor 2 Deficiency in B Lymphocytes Predisposes to Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma in Mice.
    Authors: Perez-Chacon G, Llobet D, Pardo C, Pindado J, Choi Y, Reed JC, Zapata JM
    J. Immunol., 2012-06-18;189(2):1053-61.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012-06-04;109(25):9971-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. BAFF receptor signaling aids the differentiation of immature B cells into transitional B cells following tonic BCR signaling.
    Authors: Rowland SL, Leahy KF, Halverson R
    J. Immunol., 2010-09-22;185(8):4570-81.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. TNF superfamily member, TL1A, is a potential mucosal vaccine adjuvant.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Katayama K, Yoshida T, Yamashita K, Yoshikawa T, Hiroi T, Itoh N, Kawai Y, Mayumi T, Kamada H, Tsunoda S, Tsutsumi Y
    Biochem. Biophys. Res. Commun., 2009-05-04;384(3):296-300.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  20. BAFF antagonist attenuates the development of skin fibrosis in tight-skin mice.
    Authors: Matsushita T, Fujimoto M, Hasegawa M, Matsushita Y, Komura K, Ogawa F, Watanabe R, Takehara K, Sato S
    J. Invest. Dermatol., 2007-06-21;127(12):2772-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. BAFF controls B cell metabolic fitness through a PKC beta- and Akt-dependent mechanism.
    Authors: Patke A, Mecklenbrauker I, Erdjument-Bromage H, Tempst P, Tarakhovsky A
    J. Exp. Med., 2006-10-23;203(11):2551-62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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