Home / BAFF/BLyS/TNFSF13B / Recombinant Human BAFF/BLyS/TNFSF13B Protein

Recombinant Human BAFF/BLyS/TNFSF13B Protein

Catalog #: 2149-BF
19 Citations 3 Reviews Datasheet / COA / SDS
Newer Version Available: 11403-BF
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Carrier Free

Catalog # Availability Size / Price Qty
2149-BF-010/CF
2149-BF-01M/CF

With Carrier

Catalog # Availability Size / Price Qty
2149-BF-010
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (19)
FAQs
Reviews (3)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BAFF/BLyS/TNFSF13B Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.4-2 ng/mL in the presence of goat anti‑mouse IgM µ chain.
Source
Mouse myeloma cell line, NS0-derived human BAFF/BLyS/TNFSF13B protein
MHHHHHHHHHH GGGSGGGSGGGS IEGR Human BAFF
(Ala134-Leu285)
Accession # Q9Y275.1
N-terminus C-terminus
Accession #
Q9Y275.1
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
19.6 kDa
SDS-PAGE
22-29 kDa, reducing conditions

Product Datasheets

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2149-BF (with carrier)

Product Datasheet
COA
SDS

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2149-BF/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2149-BF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2149-BF/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BAFF/BLyS/TNFSF13B

B-cell activating factor (BAFF), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a 32 kDa transmembrane glycoprotein in the TNF ligand superfamily. It is involved in multiple aspects of immune system regulation, particularly towards B cells (1, 2). Mature human BAFF consists of a 46 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 218 aa extracellular domain (ECD) with a stalk region and one TNF-like domain (3, 4). Within aa 134-285 of the ECD, human BAFF shares 72% aa sequence identity with mouse BAFF. It can be expressed as a homotrimer or as a heteromer in association with the related TNFSF member APRIL (4, 5). A 18 kDa fragment containing the TNF-like domain can be released by proteolysis between Arg133 and Ala134 (4). Soluble BAFF is stored intracellularly in neutrophils and released upon inflammatory stimulation (6). Alternative splicing generates an isoform termed deltaBAFF that lacks 19 aa between the proteolytic cleavage site and the TNF-like domain. deltaBAFF can form heteromers with BAFF and negatively regulates BAFF function (7). BAFF is produced by many hematopoietic cell types including by monocytes, macrophages, neutrophils, dendritic cells, and T cells and also by adipocytes (1, 2, 8). Both BAFF and APRIL are functional ligands for the TNF receptor superfamily members BCMA and TACI, and BAFF additionally binds and signals through BAFF R (9, 10). All three receptors are primarily expressed by B cells (10). BAFF plays a critical role in the development and survival of B lineage cells (2, 11, 12). Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity (13). Soluble BAFF is elevated in B cell malignancies, autoimmunity, and other immune disorders (1). In addition, BAFF co-stimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells (14-16). BAFF also promotes monocyte survival, proinflammatory cytokine secretion, and differentiation to macrophages (17).

References
  1. Lied, G.A. and A. Berstad (2011) Scand. J. Immunol. 73:1.
  2. Mackay, F. et al. (2010) Immunol. Rev. 237:205.
  3. Moore, P.A. et al. (1999) Science 285:260.
  4. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
  5. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  6. Scapini, P. et al. (2003) J. Exp. Med. 197:297.
  7. Gavin, A.L. et al. (2003) J. Biol. Chem. 278:38220.
  8. Alexaki, V.-I. et al. (2009) J. Immunol. 183:5948.
  9. Yu, G. et al. (2000) Nat. Immunol. 1:252.
  10. Thompson, J.S. et al. (2001) Science 293:2108.
  11. Schiemann, B. et al. (2001) Science 293:2111.
  12. Litinskiy, M.B. et al. (2002) Nat. Immunol. 3:822.
  13. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
  14. Huard, B. et al. (2001) J. Immunol. 167:6225.
  15. Sutherland, A.P.R. et al. (2005) J. Immunol. 174:5537.
  16. Walters, S. et al. (2009) J. Immunol. 182:793.
  17. Chang, S.K. et al. (2006) Blood 108:2687.
Long Name
B cell Activating Factor
Entrez Gene IDs
10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)
Alternate Names
ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4

Citations for Recombinant Human BAFF/BLyS/TNFSF13B Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Regulation of B cell functions by S-nitrosoglutathione in the EAE model
    Authors: J Kim, SMT Islam, F Qiao, AK Singh, M Khan, J Won, I Singh
    Redox Biology, 2021-06-23;45(0):102053.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. APRIL and BAFF: novel biomarkers for central nervous system lymphoma
    Authors: M Mulazzani, M Huber, S Borchard, S Langer, B Angele, E Schuh, E Meinl, M Dreyling, T Birnbaum, A Straube, U Koedel, L von Baumga
    J Hematol Oncol, 2019-10-15;12(1):102.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Condition-dependent generation of aquaporin-4 antibodies from circulating B cells in neuromyelitis optica
    Authors: R Wilson, M Makuch, AK Kienzler, J Varley, J Taylor, M Woodhall, J Palace, MI Leite, P Waters, SR Irani
    Brain, 2018-04-01;141(4):1063-1074.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease
    Authors: AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle
    MAbs, 2018-03-05;0(0):1-43.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  5. Abnormal differentiation of B cells and megakaryocytes in Roifman syndrome
    Authors: J Heremans, JE Garcia-Per, E Turro, SM Schlenner, I Casteels, R Collin, F de Zegher, D Greene, S Humblet-Ba, S Lesage, P Matthys, CJ Penkett, K Put, K Stirrups, C Thys, C Van Geet, E Van Nieuwe, C Wouters, I Meyts, K Freson, A Liston
    J. Allergy Clin. Immunol., 2018-01-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. NF-?B inducing kinase is a therapeutic target for systemic lupus erythematosus
    Authors: HD Brightbill, E Suto, N Blaquiere, N Ramamoorth, S Sujatha-Bh, EB Gogol, GM Castanedo, BT Jackson, YC Kwon, S Haller, J Lesch, K Bents, C Everett, PB Kohli, S Linge, L Christian, K Barrett, A Jaochico, LM Berezhkovs, PW Fan, Z Modrusan, K Veliz, MJ Townsend, J DeVoss, AR Johnson, R Godemann, WP Lee, CD Austin, BS McKenzie, JA Hackney, JJ Crawford, ST Staben, MH Alaoui Ism, LC Wu, N Ghilardi
    Nat Commun, 2018-01-12;9(1):179.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. EZH2 inhibition by tazemetostat results in altered dependency on B-cell activation signaling�in DLBCL
    Authors: D Brach, D Johnston-B, A Drew, T Lingaraj, V Motwani, NM Warholic, I Feldman, C Plescia, JJ Smith, RA Copeland, H Keilhack, E Chan-Peneb, SK Knutson, SA Ribich, A Raimondi, MJ Thomenius
    Mol. Cancer Ther., 2017-08-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. A TLR9-dependent checkpoint governs B cell responses to DNA-containing antigens
    Authors: VJ Sindhava, MA Oropallo, K Moody, M Naradikian, LE Higdon, L Zhou, A Myles, N Green, K Nündel, W Stohl, AM Schmidt, W Cao, S Dorta-Estr, T Kambayashi, A Marshak-Ro, MP Cancro
    J. Clin. Invest, 2017-03-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. TACI expression and signaling in chronic lymphocytic leukemia.
    Authors: Mamara, Antigoni, Germenis, Anastasi, Kompoti, Maria, Palassopoulou, Maria, Mandala, Eudokia, Banti, Anastasi, Giannakoulas, Nikolaos, Speletas, Matthaio
    J Immunol Res, 2015-04-09;2015(0):478753.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Phosphoinositide protein kinase PDPK1 is a crucial cell signaling mediator in multiple myeloma.
    Authors: Chinen Y, Kuroda J, Shimura Y, Nagoshi H, Kiyota M, Yamamoto-Sugitani M, Mizutani S, Sakamoto N, Ri M, Kawata E, Kobayashi T, Matsumoto Y, Horiike S, Iida S, Taniwaki M
    Cancer Res, 2014-09-30;74(24):7418-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Metabolic reprogramming is required for antibody production that is suppressed in anergic but exaggerated in chronically BAFF-exposed B cells.
    Authors: Caro-Maldonado, Alfredo, Wang, Ruoning, Nichols, Amanda G, Kuraoka, Masayuki, Milasta, Sandra, Sun, Lillian, Gavin, Amanda L, Abel, E Dale, Kelsoe, Garnett, Green, Douglas, Rathmell, Jeffrey
    J Immunol, 2014-03-10;192(8):3626-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Diversion of the host humoral response: a novel virulence mechanism of Haemophilus influenzae mediated via outer membrane vesicles.
    Authors: Deknuydt F, Nordstrom T, Riesbeck K
    J Leukoc Biol, 2014-02-18;95(6):983-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. H-Y antigen-binding B cells develop in male recipients of female hematopoietic cells and associate with chronic graft vs. host disease.
    Authors: Sahaf B, Yang Y, Arai S, Herzenberg L, Herzenberg L, Miklos D
    Proc Natl Acad Sci U S A, 2013-02-04;110(8):3005-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Overexpression of the autoimmunity-associated phosphatase PTPN22 promotes survival of antigen-stimulated CLL cells by selectively activating AKT.
    Blood, 2012-05-08;119(26):6278-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells.
    Authors: Zhu J, Sun L, Lin S
    J. Exp. Clin. Cancer Res., 2012-03-31;31(0):31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. BAFF is increased in renal transplant patients following treatment with alemtuzumab.
    Authors: Bloom D, Chang Z, Pauly K
    Am. J. Transplant., 2009-06-12;9(8):1835-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Neutralizing B-cell activating factor antibody improves survival and inhibits osteoclastogenesis in a severe combined immunodeficient human multiple myeloma model.
    Authors: Neri P, Kumar S, Fulciniti MT, Vallet S, Chhetri S, Mukherjee S, Tai Y, Chauhan D, Tassone P, Venuta S, Munshi NC, Hideshima T, Anderson KC, Raje N
    Clin. Cancer Res., 2007-10-01;13(19):5903-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Decreased expression of tumor necrosis factor family receptors involved in humoral immune responses in preterm neonates.
    Authors: Kaur K, Chowdhury S, Greenspan NS, Schreiber JR
    Blood, 2007-07-18;110(8):2948-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells.
    Authors: Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, Kuchen S, Lipsky PE
    J. Immunol., 2007-03-01;178(5):2872-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human BAFF/BLyS/TNFSF13B Protein

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Recombinant Human BAFF/BLyS/TNFSF13B Protein
By Anonymous on 12/12/2019
Application: In vitro bioactivity in cell culture
Reason for Rating: works in reporter assay
In vitro bioactivity in cell culture Recombinant Human BAFF/BLyS/TNFSF13B Protein 2149-BF
Recombinant Human BAFF/BLyS/TNFSF13B Protein
By Jenna Nguyen on 11/05/2018
Application: In vivo study
Recombinant Human BAFF/BLyS/TNFSF13B Protein
By Jenna Nguyen on 07/27/2018
Application: Binding assay/Protein-protein interaction