Recombinant Human BMP-10 Protein Summary
Product Specifications
Asn317-Arg424
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2926-BP
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCI containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
2926-BP/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCI. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: BMP-10
BMP-10, along with BMP-9, GDF-5, -6, and -7, belongs to a subgroup of TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors (1-3). Proteolytic removal of the propeptide from the 60 kDa proprotein yields a 12 kDa mature BMP-10 which forms disulfide-linked
non-glycosylated homodimers (4, 5). Mature human BMP-10 shares 98% amino acid sequence identity with mouse and rat BMP-10 and 49% - 63% with human BMP-9, GDF-5, -6, and -7. BMP-10 is critical for the proper development of the heart but is not expressed until after cardiac patterning or looping are completed (6-8). BMP-10 production appears at the onset of trabeculation and chamber formation and is restricted to the right atrium in the adult heart (6-8). Homozygous BMP-10 knockout mice die in utero due to arrested cardiac development (7). BMP-10 is required for the expression of the cardiogenic transcription factors NKX2.5 and MEF2C in developing myocardium and the growth of embryonic cardiomyocytes (7, 10). NKX2.5 itself negatively regulates BMP-10 expression in cardiac myocytes (10). Multiple human congenital heart defects result from mutations in NKX2.5 and require BMP-10 expression (10). In mice, genetic knockout of ErbB leads to a similar phenotype but appears not to involve BMP-10, and knockout of the calcium channel subunit FKBP12 induces BMP-10 over-expression (7). BMP-10 in the postnatal heart promotes increased cardiomyocyte and heart size (8). BMP-10 has been shown to signal through ALK-1, BMPR-IA, BMPR-IB, and BMPR-II in transfectants and non-cardiac cell lines (4, 5). A functional BMP-10 receptor in the heart has not yet been identified, although deletion of BMPR-IA causes similar cardiac morphogenetic abnormalities (11). In dermal endothelial cells, BMP-10 induces migration, proliferation, and gene expression typically associated with ALK-1 (5).
- Chen, D. et al. (2004) Growth Factors 22:233.
- Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
- Schneider, M.D. et al. (2003) Cytokine Growth Factor Rev. 14:1.
- Mazerbourg, S. et al. (2005) J. Biol. Chem. 280:32122.
- David, L. et al. (2007) Blood 109:1953.
- Neuhaus, H. et al. (1999) Mech. Dev. 80:181.
- Chen, H. et al. (2004) Development 131:2219.
- Chen, H. et al. (2006) J. Biol. Chem. 281:27481.
- Srivastava, D. and E.N. Olson (2000) Nature 407:221.
- Pashmforoush, M. et al. (2004) Cell 117:373.
- Gaussin, V. et al. (2002) Proc. Natl. Acad. Sci. 99:2878.
Citations for Recombinant Human BMP-10 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Shear Stress and Sub-Femtomolar Levels of Ligand Synergize to Activate ALK1 Signaling in Endothelial Cells
Authors: Cheng, YW;Anzell, AR;Morosky, SA;Schwartze, TA;Hinck, CS;Hinck, AP;Roman, BL;Davidson, LA;
Cells
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Oxidative stress-induced MMP- and gamma-secretase-dependent VE-cadherin processing is modulated by the proteasome and BMP9/10
Authors: C Ivaldo, M Passalacqu, AL Furfaro, C d'Abramo, S Ruiz, PK Chatterjee, CN Metz, M Nitti, P Marambaud
Scientific Reports, 2023-01-11;13(1):597.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Reciprocal SOX2 regulation by SMAD1-SMAD3 is critical for anoikis resistance and metastasis in cancer
Authors: Z Shonibare, M Monavarian, K O'Connell, D Altomare, A Shelton, S Mehta, R Jaskula-Sz, R Phaeton, MD Starr, R Whitaker, A Berchuck, AB Nixon, RC Arend, NY Lee, CR Miller, N Hempel, K Mythreye
Oncogene, 2022-07-26;40(4):111066.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Crystal structures of BMPRII extracellular domain in binary and ternary receptor complexes with BMP10
Authors: J Guo, B Liu, M Thorikay, M Yu, X Li, Z Tong, RM Salmon, RJ Read, P Ten Dijke, NW Morrell, W Li
Nature Communications, 2022-05-03;13(1):2395.
Species: Human
Sample Types: Recombinant Proteins
Applications: Surface Plasmon Resonance -
Functionally diverse heteromeric traps for ligands of the transforming growth factor-beta superfamily
Authors: R Kumar, AV Grinberg, H Li, TH Kuo, D Sako, L Krishnan, K Liharska, J Li, R Grenha, MC Maguire, SD Briscoe, RS Pearsall, BR Herrin, RNVS Suragani, R Castonguay
Scientific Reports, 2021-09-15;11(1):18341.
Species: Human
Sample Types: Recombinant Proteins
Applications: Bioassay -
Bone Morphogenetic Protein 9 Regulates Early Lymphatic-Specified Endothelial Cell Expansion during Mouse Embryonic Stem Cell Differentiation
Authors: M Subileau, G Merdzhanov, D Ciais, V Collin-Fau, JJ Feige, S Bailly, D Vittet
Stem Cell Reports, 2018-12-27;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
A heterodimer formed by bone morphogenetic protein 9 (BMP9) and BMP10 provides most BMP biological activity in plasma
Authors: E Tillet, M Ouarné, A Desroches-, C Mallet, M Subileau, R Didier, A Lioutsko, G Belthier, JJ Feige, S Bailly
J. Biol. Chem., 2018-05-22;0(0):.
Applications: Western Blot -
Bone morphogenetic protein (BMP) 9 and BMP10 enhance tumor necrosis factor-?-induced monocyte recruitment to the vascular endothelium mainly via activin receptor-like kinase 2
Authors: CG Mitrofan, SL Appleby, GB Nash, Z Mallat, ER Chilvers, PD Upton, NW Morrell
J. Biol. Chem., 2017-06-23;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Mutual interaction between endothelial cells and mural cells enhances BMP9 signaling in endothelial cells
Authors: Y Tachida, N Izumi, T Sakurai, H Kobayashi
Biol Open, 2017-03-15;6(3):370-380.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
BMP9 and BMP10 are necessary for proper closure of the ductus arteriosus.
Authors: Levet S, Ouarne M, Ciais D, Coutton C, Subileau M, Mallet C, Ricard N, Bidart M, Debillon T, Faravelli F, Rooryck C, Feige J, Tillet E, Bailly S
Proc Natl Acad Sci U S A, 2015-06-08;112(25):E3207-15.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A BMP7 Variant Inhibits Tumor Angiogenesis In Vitro and In Vivo through Direct Modulation of Endothelial Cell Biology.
Authors: Tate C, Mc Entire J, Pallini R, Vakana E, Wyss L, Blosser W, Ricci-Vitiani L, D'Alessandris Q, Morgante L, Giannetti S, Larocca L, Todaro M, Benfante A, Colorito M, Stassi G, De Maria R, Rowlinson S, Stancato L
PLoS ONE, 2015-04-28;10(4):e0125697.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
BMP9 and BMP10 are critical for postnatal retinal vascular remodeling.
Blood, 2012-05-07;119(25):6162-71.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
J. Biol. Chem., 2011-07-07;286(34):30034-46.
Species: Human, Mouse
Sample Types: Recombinant Protein, Whole Cells
Applications: Bioassay, Surface Plasmon Resonance -
A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
Authors: Herrera B, Inman GJ
BMC Cell Biol., 2009-03-19;10(0):20.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Targeting of bone morphogenetic protein growth factor complexes to fibrillin.
Authors: Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY
J. Biol. Chem., 2008-03-13;283(20):13874-88.
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay, Surface Plasmon Resonance
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Stimulated epithelial cell line with BMP-10 protein for 30 min to induce pSmad 1/5/8 phosphorylation
