Recombinant Human Integrin alpha 5 beta 1 Protein, CF Summary
Product Specifications
| Human Integrin alpha 5 (Phe42-Tyr995) Accession # P08648 |
acidic tail |
| Human Integrin beta 1 (Gln21-Asp728) Accession # P05556 |
basic tail |
| N-terminus | C-terminus |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3230-A5
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Integrin alpha 5 beta 1
Integrin alpha 5/ beta 1, also known as VLA-5, is a widely expressed non-covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit. alpha 5/ beta 1 functions in cell adhesion, migration, activation, and matrix fibrillogenesis (1, 2). The human Integrin alpha 5/CD49e cDNA encodes a 1049 amino acid (aa) precursor that includes a 41 aa signal sequence, a 954 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 28 aa cytoplasmic domain. The ECD contains seven FG-GAP repeats and nine internal disulfide bonds (3). Within the ECD, human alpha 5 shares 90% and 46% aa sequence identity with mouse alpha 5 and human alpha 8, respectively, and less than 30% aa sequence identitity with other human alpha chains. The human Integrin beta 1/CD29 cDNA encodes a 798 aa precursor that includes a 20 aa signal sequence, a 708 aa ECD, a 23 aa transmembrane segment, and a 47 aa cytoplasmic domain. The ECD contains one vWF-A domain, four Cys-rich repeats, and 29 internal disulfide bonds (3). Five alternate splice forms of the cytoplasmic domain vary by 12 to 48 aa. Within the ECD, human beta 1 shares 92-96% aa sequence identity with rat, bovine, mouse, and feline beta 1. It shares 35-45% aa sequence identity with other beta chains. alpha 5/ beta 1 binds fibronectin in both RGD-dependent and -independent manners (4, 5). alpha 5/ beta 1 is up‑regulated on tumor vasculature and promotes angiogenesis (6, 7). This is accomplished in part by a constitutive association in cis of alpha 5/ beta 1 with VEGFR3 and Tie2, a requirement for optimal activation of those receptors (8, 9). alpha 5/ beta 1 interacts with a variety of other proteins, including HER2, uPAR, Galectin‑1, CTGF, and thrombin-cleaved Osteopontin (5, 10-13). alpha 5/ beta 1 also functions on some hematopoietic and neuronal stem cells (14, 15).
- Mao, Y. and J.E. Schwarzbauer (2005) Matrix Biol. 24:389.
- Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
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- Kim, S. et al. (2000) Am. J. Pathol. 156:1345.
- Zhang, X. et al. (2004) J. Cell. Physiol. 202:205.
- Cascone, I. et al. (2005) J. Cell Biol. 170:993.
- Kuwada, S.K. et al. (2005) J. Biol. Chem. 280:19027.
- Fischer, C. et al. (2005) J. Biol. Chem. 280:37266.
- Gao, R. and D.R. Brigstock (2005) Gut 129:1019
- Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
- Carstanjen, D. et al. (2005) Transfusion 45:1192.
- Gibson, R.M. et al. (2005) Mol. Cell. Neurosci. 28:588.
Citations for Recombinant Human Integrin alpha 5 beta 1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Identification of AXL as a co-receptor for human parvovirus B19 infection of human erythroid progenitors
Authors: K Ning, W Zou, P Xu, F Cheng, EY Zhang, A Zhang-Chen, S Kleiboeker, J Qiu
Science Advances, 2023-01-11;9(2):eade0869.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Angiopoietin-like 3-derivative LNA043 for cartilage regeneration in osteoarthritis: a randomized phase 1 trial
Authors: N Gerwin, C Scotti, C Halleux, M Fornaro, J Elliott, Y Zhang, K Johnson, J Shi, S Walter, Y Li, C Jacobi, N Laplanche, M Belaud, J Paul, G Glowacki, T Peters, KA Wharton, I Vostiar, F Polus, I Kramer, S Guth, A Seroutou, S Choudhury, D Laurent, J Gimbel, J Goldhahn, M Schieker, S Brachat, R Roubenoff, M Kneissel
Nature Medicine, 2022-12-01;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Immunoprecipitation -
An innovative strategy to identify new targets for delivering antibodies to the brain has led to the exploration of the integrin family
Authors: C Cegarra, B Cameron, C Chaves, T Dabdoubi, TM Do, B Genêt, V Roudières, Y Shi, P Tchepikoff, D Lesuisse
PLoS ONE, 2022-09-15;17(9):e0274667.
Species: Human
Sample Types: Cell Lysates
Applications: ELISA Capture -
Receptor binding domain of SARS-CoV-2 is a functional alphav-integrin agonist
Authors: EG Norris, XS Pan, DC Hocking
bioRxiv : the preprint server for biology, 2022-04-11;0(0):.
Species: N/A
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR -
Angiopoietin-like 3-derivative LNA043 for cartilage regeneration in osteoarthritis: a randomized phase 1 trial
Authors: N Gerwin, C Scotti, C Halleux, M Fornaro, J Elliott, Y Zhang, K Johnson, J Shi, S Walter, Y Li, C Jacobi, N Laplanche, M Belaud, J Paul, G Glowacki, T Peters, KA Wharton, I Vostiar, F Polus, I Kramer, S Guth, A Seroutou, S Choudhury, D Laurent, J Gimbel, J Goldhahn, M Schieker, S Brachat, R Roubenoff, M Kneissel
Nature Medicine, 2022;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Immunoprecipitation -
The Cell Surface Hyaluronidase TMEM2 Regulates Cell Adhesion and Migration via Degradation of Hyaluronan at Focal Adhesion Sites
Authors: F Irie, Y Tobisawa, A Murao, H Yamamoto, C Ohyama, Y Yamaguchi
The Journal of Biological Chemistry, 2021-02-26;0(0):100481.
Species: Human
Sample Types: Protein
Applications: Bioassay -
Interaction of Pregnancy-Specific Glycoprotein 1 With Integrin alpha5beta1 Is a Modulator of Extravillous Trophoblast Functions
Authors: S Rattila, CEE Dunk, M Im, O Grichenko, Y Zhou, M Yanez-Mo, SM Blois, KM Yamada, O Erez, N Gomez-Lope, SJ Lye, B Hinz, R Romero, M Cohen, G Dveksler
Cells, 2019-10-31;8(11):.
Species: Human
Sample Types:
Applications: ELISA Standard -
ITGA5 inhibition in pancreatic stellate cells attenuates desmoplasia and potentiates efficacy of chemotherapy in pancreatic cancer
Authors: PR Kuninty, R Bansal, SWL De Geus, DF Mardhian, J Schnittert, J van Baarle, G Storm, MF Bijlsma, HW van Laarho, JM Metselaar, PJK Kuppen, AL Vahrmeijer, A Östman, CFM Sier, J Prakash
Sci Adv, 2019-09-04;5(9):eaax2770.
Species: Human
Sample Types: Peptide
Applications: Bioassay -
CagY-Dependent Regulation of Type IV Secretion in Helicobacter pylori Is Associated with Alterations in Integrin Binding
Authors: EC Skoog, VA Morikis, ME Martin, GA Foster, LP Cai, LM Hansen, B Li, JA Gaddy, SI Simon, JV Solnick
MBio, 2018-05-15;9(3):.
Applications: Bioassay -
Therapeutic potential of an anti-angiogenic multimodal biomimetic peptide in hepatocellular carcinoma
Authors: MA Barbhuiya, AC Mirando, BW Simons, G Lemtiri-Ch, JJ Green, AS Popel, NB Pandey, PT Tran
Oncotarget, 2017-09-21;8(60):101520-101534.
Species: Human
Sample Types: Peptide
Applications: Bioassay -
Systematic site-directed mutagenesis of the Helicobacter pylori CagL protein of the Cag type IV secretion system identifies novel functional domains
Sci Rep, 2016-12-06;6(0):38101.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Developmet -
Integrin alpha3beta1 Binding to Fibronectin Is Dependent on the Ninth Type III Repeat.
Authors: Brown A, Dysart M, Clarke K, Stabenfeldt S, Barker T
J Biol Chem, 2015-08-28;290(42):25534-47.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Snail modulates the assembly of fibronectin via alpha5 integrin for myocardial migration in zebrafish embryos.
Authors: Qiao L, Gao H, Zhang T, Jing L, Xiao C, Xiao Y, Luo N, Zhu H, Meng W, Xu H, Mo X
Sci Rep, 2014-03-26;4(0):4470.
Species: Zebrafish
Sample Types: Embryo
Applications: In Vivo -
LGALS3BP, lectin galactoside-binding soluble 3 binding protein, promotes oncogenic cellular events impeded by antibody intervention.
Authors: Stampolidis P, Ullrich A, Iacobelli S
Oncogene, 2013-12-23;34(1):39-52.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods: a FeCl3-induced thrombosis model in mice.
Authors: Kwon I, Hong S, Kim Y, Nam H, Kang S, Yang S, Heo J
PLoS ONE, 2013-11-18;8(11):e81165.
Applications: Binding Assay -
Integrin alpha2beta1 mediates tyrosine phosphorylation of vascular endothelial cadherin induced by invasive breast cancer cells.
Authors: Haidari M, Zhang W, Caivano A, Chen Z, Ganjehei L, Mortazavi A, Stroud C, Woodside D, Willerson J, Dixon R
J Biol Chem, 2012-07-25;287(39):32981-92.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
PEGylated liposomal doxorubicin targeted to alpha5beta1-expressing MDA-MB-231 breast cancer cells.
Authors: Shroff K, Kokkoli E
Langmuir, 2012-02-29;28(10):4729-36.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Heterodimeric integrin complexes containing beta1-integrin promote internalization and lethality of anthrax toxin.
Authors: Martchenko M, Jeong SY, Cohen SN
Proc. Natl. Acad. Sci. U.S.A., 2010-08-16;107(35):15583-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Efficient delivery of angiostatin K1-5 into tumors following insertion of an NGR peptide into adenovirus capsid.
Authors: Jullienne B, Vigant F, Muth E, Chaligne R, Bouquet C, Giraudier S, Perricaudet M, Benihoud K
Gene Ther., 2009-12-01;16(12):1405-15.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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What is the amino acid sequence of the acidic and basic tails?
Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.
Reviews for Recombinant Human Integrin alpha 5 beta 1 Protein, CF
Average Rating: 4.3 (Based on 3 Reviews)
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Reason for Rating: The product was easy to dissolve and induced an expected response in cell culture.
Integrin was used to stimulate a pro-inflammatory activity on rats’ synoviocytes. For this, primary synoviocytes were isolated from rats’ articulations and treated with the integrin. Results showed a pro-inflammatory production of IL-1ra, IL-6, and TNF-a.
