Recombinant Human Notch-1 Fc Chimera Protein, CF Summary
Product Specifications
| Human Notch-1 (Ala19 - Gln526) Accession # P46531 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3647-TK
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Notch-1
Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes (1 - 3). Notch signaling is important for maintaining stem cells and inducing differentiation, especially in the nervous system and lymphoid tissues (2 - 4). Notch can specify binary cell fates; for example, promoting T- over B-cell development from a common precursor (2). More than 50% of human T-lineage acute lymphoblastic leukemia (T-ALL) have activating mutations of Notch1 (1, 5). Human Notch-1 is synthesized as a 2556 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1718 aa extracellular domain (ECD) with 36 EGF-like repeats and three Lin-12/Notch repeats (LNR), a 23 aa transmembrane (TM) segment and a 785 aa cytoplasmic domain containing six ankyrin repeats, a glutamine-rich domain and a PEST sequence. The 11th and 12th EGF-like repeats bind ligands including Jagged and Delta-like families in humans (6). O-fucosylation by Fringe family members at a site within this region can inhibit the interaction of Notch with Jagged ligands, thereby promoting Delta-like ligand interactions (7). Notch-1 receptor undergoes post-translational furin-type proteolytic cleavage, forming a heterodimer through interaction of a hydrophobic area C-terminal to the LNR on the 1647 aa ligand-binding extracellular region with the 891 aa transmembrane/cytoplasmic portion (8, 9). Upon ligand binding, additional sequential proteolysis by TNF-converting enzyme (ADAM-17) and the presenilin-dependent gamma -secretase results in the release of the Notch intracellular domain (NICD) which translocates into the nucleus, activating transcription of Notch-responsive genes (10). Human Notch-1 ECD aa 19 - 526, including the first 13 EGF repeats, shows 91% aa identity with corresponding regions of mouse and rat, 89% with canine, and 79% with chicken Notch-1. This region also exhibits 60% aa identity with human Notch-2 and Notch-3.
- Ellisen, L. W. et al. (1991) Cell 66:649.
- Dumortier, A. et al. (2005) Int. J. Hematol. 82:277.
- Yoon, K. and N. Gaiano (2005) Nat. Neurosci. 8:709.
- Androutsellis-Theotokis, A. et al. (2006) Nature 442:823.
- Weng, A. P. et al. (2004) Science 306:269.
- Hambleton, S. et al. (2004) Structure 12:2173.
- Yang, L. et al. (2005) Mol. Biol. Cell 16:927.
- Sanchez-Irizarry, C. et al. (2004) Mol. Cell. Biol. 24:9265.
- Logeat, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8108.
- Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151.
Citations for Recombinant Human Notch-1 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Generation of synthetic antibody fragments with optimal complementarity determining region lengths for Notch-1 recognition
Authors: BV Maruthacha, K Barreto, D Hogan, A Kusalik, CR Geyer
Frontiers in Microbiology, 2022-08-03;13(0):931307.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
ABT-165, a Dual Variable Domain Immunoglobulin (DVD-Ig) Targeting DLL4 and VEGF, Demonstrates Superior Efficacy and Favorable Safety Profiles in Preclinical Models
Authors: Y Li, JA Hickson, DJ Ambrosi, DL Haasch, KD Foster-Duk, LJ Eaton, EL Digiammari, SC Panchal, F Jiang, SR Mudd, C Zhang, SS Akella, W Gao, SL Ralston, L Naumovski, J Gu, SE Morgan-Lap
Mol. Cancer Ther., 2018-03-28;0(0):.
Applications: ELISA (Standard) -
Intrinsic selectivity of Notch 1 for Delta-like 4 over Delta-like 1.
Authors: Andrawes M, Xu X, Liu H, Ficarro S, Marto J, Aster J, Blacklow S
J Biol Chem, 2013-07-09;288(35):25477-89.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Inhibition of Dll4-mediated signaling induces proliferation of immature vessels and results in poor tissue perfusion.
Authors: Scehnet JS, Jiang W, Kumar SR, Krasnoperov V, Trindade A, Benedito R, Djokovic D, Borges C, Ley EJ, Duarte A, Gill PS
Blood, 2007-02-20;109(11):4753-60.
Species: Human
Sample Types: Recombinant Protein, Whole Cells
Applications: Binding Assay, Bioassay
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