Home / PDGF-BB / Recombinant Rat PDGF-BB Protein

Recombinant Rat PDGF-BB Protein

Catalog #: 520-BB
44 Citations 3 Reviews Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
520-BB-050/CF

With Carrier

Catalog # Availability Size / Price Qty
520-BB-050
Graph showing dose-dependent bioactivity of rat PDGF-BB protein
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Product Details
Citations (44)
FAQs
Supplemental Products
Reviews (3)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat PDGF-BB Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 1.5‑6 ng/mL.
Source
E. coli-derived rat PDGF-BB protein
Ser74-Thr182
Accession #
Q05028.1
N-terminal Sequence
Analysis
Ser74
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
25 kDa

Product Datasheets

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520-BB (with carrier)

Product Datasheet
COA
SDS

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520-BB/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

520-BB

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

520-BB/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Graph showing dose-dependent bioactivity of rat PDGF-BB protein View Larger

Recombinant rat PDGF-BB (520-BB) induces proliferation in the NR6R-3T3 mouse fibroblast cell line. The ED50 for this effect is 1.5-6 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGF-BB

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the
alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production; temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

References
  1. Raines, E.W. et al. (1990) Platelet-derived growth factor in Handbook of Experimental Pharmacology, Peptide growth factors and their receptors, M.B. Sporn and A.B. Roberts, eds. p. 173.
Long Name
Platelet-derived Growth Factor BB
Entrez Gene IDs
5155 (Human); 24628 (Rat)
Alternate Names
c-sis;IBGC5;PDGF2;PDGF-2;PDGFB;Platelet-derived growth factor subunit B;SIS;SSV; PDGFBB; PDGF-BB

Citations for Recombinant Rat PDGF-BB Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
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  1. Sigma-1 Receptor Agonist Fluvoxamine Ameliorates Fibrotic Response of Trabecular Meshwork Cells
    Authors: Hodrea, J;Tran, MN;Besztercei, B;Medveczki, T;Szabo, AJ;Őrfi, L;Kovacs, I;Fekete, A;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Combination of PDGF-BB and adipose-derived stem cells accelerated wound healing through modulating PTEN/AKT pathway
    Authors: F Lai, S Dai, Y Zhao, Y Sun
    Injury, 2023-02-13;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Ant-Neointimal Formation Effects of SLC6A6 in Preventing Vascular Smooth Muscle Cell Proliferation and Migration via Wnt/beta-Catenin Signaling
    Authors: Z Rong, F Li, R Zhang, S Niu, X Di, L Ni, C Liu
    International Journal of Molecular Sciences, 2023-02-03;24(3):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Integrin beta1, PDGFRbeta, and type II collagen are essential for meniscus regeneration by synovial mesenchymal stem cells in rats
    Authors: T Kitahashi, R Kogawa, K Nakamura, I Sekiya
    Scientific Reports, 2022-08-19;12(1):14148.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Temporal control of PDGFRalpha regulates the fibroblast-to-myofibroblast transition in wound healing
    Authors: L Yao, BH Rathnakar, HR Kwon, H Sakashita, JH Kim, A Rackley, JJ Tomasek, WL Berry, LE Olson
    Cell Reports, 2022-08-16;40(7):111192.
    Species: Mouse
    Sample Types: Serum
    Applications: Bioassay
  6. Recombinant platelet-derived growth factor-BB alleviates osteoarthritis in a rat model by decreasing chondrocyte apoptosis in vitro and in vivo
    Authors: P Zhu, Z Wang, Z Sun, B Liao, Y Cai
    Journal of Cellular and Molecular Medicine, 2021-07-11;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Endosomal mTORC2 Is Required for Phosphoinositide-Dependent AKT Activation in Platelet-Derived Growth Factor-Stimulated Glioma Cells
    Authors: S Kim, S Heo, J Brzostowsk, D Kang
    Cancers, 2021-05-16;13(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Improved Recovery from Liver Fibrosis by Crenolanib
    Authors: D Reichert, L Adolph, JP Köhler, T Buschmann, T Luedde, D Häussinger, C Kordes
    Cells, 2021-04-04;10(4):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Ketogenic Diet Enhances the Cholesterol Accumulation in Liver and Augments the Severity of CCl4 and TAA-Induced Liver Fibrosis in Mice
    Authors: YJ Liao, YH Wang, CY Wu, FY Hsu, CY Chien, YC Lee
    International Journal of Molecular Sciences, 2021-03-13;22(6):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  10. PDGF Receptor Alpha Signaling Is Key for M�ller Cell Homeostasis Functions
    Authors: N Díaz-Lezam, A Wolf, S Koch, AM Pfaller, J Biber, X Guillonnea, T Langmann, A Grosche
    International Journal of Molecular Sciences, 2021-01-25;22(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  11. Genetic analyses in mouse fibroblast and melanoma cells demonstrate novel roles for PDGF-AB ligand and PDGF receptor alpha
    Authors: JL Kadrmas, MC Beckerle, M Yoshigi
    Sci Rep, 2020-11-09;10(1):19303.
    Species: Rat
    Sample Types: Cell Lysates, Whole Cells
    Applications: Bioassay, Western Blot
  12. Periprocedural Hydrogen Sulfide Therapy Improves Vascular Remodeling and Attenuates Vein Graft Disease
    Authors: P Kip, M Tao, KM Trocha, MR MacArthur, HAB Peters, SJ Mitchell, CG Mann, TJ Sluiter, J Jung, S Patterson, PHA Quax, MR de Vries, JR Mitchell, C Keith Ozak
    J Am Heart Assoc, 2020-11-04;0(0):e016391.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  13. Gsta4 controls apoptosis of differentiating adult oligodendrocytes during homeostasis and remyelination via the mitochondria-associated Fas-Casp8-Bid-axis
    Authors: KE Carlström, K Zhu, E Ewing, IE Krabbendam, RA Harris, AM Falcão, M Jagodic, G Castelo-Br, F Piehl
    Nat Commun, 2020-08-13;11(1):4071.
    Species: Rat
    Sample Types: Tissue Homogenates, Whole Cells
    Applications: Bioassay, Cell Culture
  14. Long-Term Exposure to Low-Dose Di-(2-ethylhexyl) Phthalate Impairs Cholesterol Metabolism in Hepatic Stellate Cells and Exacerbates Liver Librosis
    Authors: CY Lee, FM Suk, YC Twu, YJ Liao
    Int J Environ Res Public Health, 2020-05-27;17(11):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Heat shock protein 90 inhibitor AUY922 attenuates platelet-derived growth factor-BB-induced migration and proliferation of vascular smooth muscle cells
    Authors: J Kim, KP Lee, BS Kim, SJ Lee, BS Moon, S Baek
    Korean J. Physiol. Pharmacol., 2020-05-01;24(3):241-248.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Reducing Th2 inflammation through neutralizing IL-4 antibody rescues myelination in IUGR rat brain
    Authors: AE Zanno, MA Romer, L Fox, T Golden, L Jaeckle-Sa, RA Simmons, JB Grinspan
    J Neurodev Disord, 2019-12-16;11(1):34.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Ipragliflozin-induced adipose expansion inhibits cuff-induced vascular remodeling in mice
    Authors: K Mori, K Tsuchiya, S Nakamura, Y Miyachi, K Shiba, Y Ogawa, K Kitamura
    Cardiovasc Diabetol, 2019-06-24;18(1):83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Medial artery calcification increases neointimal hyperplasia after balloon injury
    Authors: AP Marshall, W Luo, XL Wang, T Lin, Y Cai, RJ Guzman
    Sci Rep, 2019-06-03;9(1):8193.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Growth factor signaling regulates mechanical nociception in flies and vertebrates
    Authors: R Lopez-Bell, S Puig, PJ Huang, CR Tsai, HN Turner, MJ Galko, HB Gutstein
    J. Neurosci., 2019-05-28;0(0):.
    Species: Rat
    Sample Types: In Vivo
    Applications: Bioassay, In Vivo
  20. Cantharidin attenuates the proliferation and migration of vascular smooth muscle cells through suppressing inflammatory response
    Authors: L Qiu, C Xu, H Jiang, W Li, S Tong, H Xia
    Biol. Pharm. Bull., 2018-11-03;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Bone marrow-derived mesenchymal stem cells inhibit vascular smooth muscle cell proliferation and neointimal hyperplasia after arterial injury in rats
    Authors: Y Iso, S Usui, M Toyoda, JL Spees, A Umezawa, H Suzuki
    Biochem Biophys Rep, 2018-10-24;16(0):79-87.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Naked physically synthesized gold nanoparticles affect migration, mitochondrial activity, and proliferation of vascular smooth muscle cells
    Authors: HM Lo, MC Ma, JM Shieh, HL Chen, WB Wu
    Int J Nanomedicine, 2018-05-29;13(0):3163-3176.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Dual Role of CREB in The Regulation of VSMC Proliferation: Mode of Activation Determines Pro- or Anti-Mitogenic Function
    Authors: C Hudson, TE Kimura, A Duggirala, GB Sala-Newby, AC Newby, M Bond
    Sci Rep, 2018-03-20;8(1):4904.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  24. 4-Hydroxybenzaldehyde accelerates acute wound healing through activation of focal adhesion signalling in keratinocytes
    Authors: CW Kang, YE Han, J Kim, JH Oh, YH Cho, EJ Lee
    Sci Rep, 2017-10-27;7(1):14192.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  25. Homer binds to Orai1 and TRPC channels in the neointima and regulates vascular smooth muscle cell migration and proliferation
    Authors: S Jia, M Rodriguez, AG Williams, JP Yuan
    Sci Rep, 2017-07-11;7(1):5075.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Wedelolactone, a plant coumarin, prevents vascular smooth muscle cell proliferation and injury-induced neointimal hyperplasia through Akt and AMPK signaling
    Authors: L Peng, X Huang, X Jin, Z Jing, L Yang, Y Zhou, J Ren, Y Zhao
    Exp. Gerontol., 2017-06-17;96(0):73-81.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Src-homology protein tyrosine phosphatase-1 agonist, SC-43, reduces liver fibrosis
    Authors: TH Su, CW Shiau, P Jao, NJ Yang, WT Tai, CJ Liu, TC Tseng, HC Yang, CH Liu, KW Huang, TC Hu, YJ Huang, YM Wu, LJ Chen, PJ Chen, DS Chen, KF Chen, JH Kao
    Sci Rep, 2017-05-11;7(1):1728.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Responses of adventitial CD34(+) vascular wall-resident stem/progenitor cells and medial smooth muscle cells to carotid injury in rats
    Authors: Jian Song
    Exp. Mol. Pathol., 2016-11-14;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Activation of Protein Kinase G (PKG) Reduces Neointimal Hyperplasia, Inhibits Platelet Aggregation, and Facilitates Re-endothelialization
    Sci Rep, 2016-11-11;6(0):36979.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Smooth Muscle Cell Pro-angiogenic Phenotype Induced by Cyclopentenyl Cytosine Promotes Endothelial Cell Proliferation and Migration
    Authors: Shi-You Chen
    J. Biol. Chem., 2016-11-07;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  31. A 3D Poly(ethylene glycol)-based Tumor Angiogenesis Model to Study the Influence of Vascular Cells on Lung Tumor Cell Behavior
    Sci Rep, 2016-09-06;6(0):32726.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Epothilones Suppress Neointimal Thickening in the Rat Carotid Balloon-Injury Model by Inducing Vascular Smooth Muscle Cell Apoptosis through p53-Dependent Signaling Pathway
    PLoS ONE, 2016-05-24;11(5):e0155859.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Sphingosine-1-phosphate mediates epidermal growth factor-induced muscle satellite cell activation.
    Authors: Nagata Y, Ohashi K, Wada E, Yuasa Y, Shiozuka M, Nonomura Y, Matsuda R
    Exp Cell Res, 2014-06-21;326(1):112-24.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Systemic sclerosis immunoglobulin induces growth and a pro-fibrotic state in vascular smooth muscle cells through the epidermal growth factor receptor.
    Authors: Arts M, Baron M, Chokr N, Fritzler M, Servant M
    PLoS ONE, 2014-06-13;9(6):e100035.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  35. TGFbeta-mediated suppression of CD248 in non-cancer cells via canonical Smad-dependent signaling pathways is uncoupled in cancer cells.
    Authors: Suresh Babu S, Valdez Y, Xu A, O'Byrne A, Calvo F, Lei V, Conway E
    BMC Cancer, 2014-02-20;14(0):113.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Endothelial dysfunction exacerbates renal interstitial fibrosis through enhancing fibroblast Smad3 linker phosphorylation in the mouse obstructed kidney.
    Authors: Sun Y, Qu X, Li X, Nikolic-Paterson D, Li J
    PLoS ONE, 2013-12-31;8(12):e84063.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  37. PSCs and GLP-1R: occurrence in normal pancreas, acute/chronic pancreatitis and effect of their activation by a GLP-1R agonist.
    Authors: Nakamura T, Ito T, Uchida M, Hijioka M, Igarashi H, Oono T, Kato M, Nakamura K, Suzuki K, Jensen R, Takayanagi R
    Lab Invest, 2013-11-11;94(1):63-78.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  38. A lymphocyte-dependent mode of action for imatinib mesylate in experimental pulmonary hypertension.
    Authors: Ormiston M, Deng Y, Rundle N, Bendjelloul F, Tsoporis J, Parker T, Stewart D, Courtman D
    Am J Pathol, 2013-03-19;182(5):1541-51.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  39. TGF beta1-Induced Baf60c Regulates both Smooth Muscle Cell Commitment and Quiescence.
    Authors: Sohni A, Mulas F, Ferrazzi F, Luttun A, Bellazzi R, Huylebroeck D, Ekker S, Verfaillie C
    PLoS ONE, 2012-10-26;7(10):e47629.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  40. A naturally occurring carotenoid, lutein, reduces PDGF and H2O2 signaling and compromised migration in cultured vascular smooth muscle cells.
    Authors: Lo HM, Tsai YJ, Du WY, Tsou CJ, Wu WB
    J. Biomed. Sci., 2012-02-08;19(0):18.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Endogenous angiogenesis inhibitor vasohibin1 exhibits broad-spectrum antilymphangiogenic activity and suppresses lymph node metastasis.
    Authors: Heishi T, Hosaka T, Suzuki Y, Miyashita H, Oike Y, Takahashi T, Nakamura T, Arioka S, Mitsuda Y, Takakura T, Hojo K, Matsumoto M, Yamauchi C, Ohta H, Sonoda H, Sato Y
    Am. J. Pathol., 2010-02-04;176(4):1950-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  42. Imatinib promotes osteoblast differentiation by inhibiting PDGFR signaling and inhibits osteoclastogenesis by both direct and stromal cell-dependent mechanisms.
    Authors: O'Sullivan S, Naot D, Callon K, Porteous F, Horne A, Wattie D, Watson M, Cornish J, Browett P, Grey A
    J. Bone Miner. Res., 2007-11-01;22(11):1679-89.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  43. Lin(-)CD34(-) bone marrow cells from adult mice can differentiate into neural-like cells.
    Authors: Li Q, Hosaka N, Cui W, Wang X, Cui Y, Cui Y, Song C, Li Q, Ryu T, Fan T, Kawamoto K, Ikehara S
    Neurosci. Lett., 2006-09-25;408(1):51-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  44. Ligand stimulation of CD155alpha inhibits cell adhesion and enhances cell migration in fibroblasts.
    Authors: Oda T, Ohka S, Nomoto A
    Biochem. Biophys. Res. Commun., 2004-07-09;319(4):1253-64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Rat PDGF-BB Protein

Average Rating: 5 (Based on 3 Reviews)

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Recombinant Rat PDGF-BB Protein
By Anonymous on 05/20/2023
Application: CellProlif

PDGF-BB Protein promote cell differentiation

Cell Proliferation Recombinant Rat PDGF-BB Protein 520-BB
Recombinant Rat PDGF-BB Protein
By Anonymous on 03/01/2022
Application: Stem/Immune cell maintenance or differentiation
Reason for Rating: Rat PDGF-BB Protein was use to promote the formation of neural cells.
Stem/Immune cell maintenance or differentiation Recombinant Rat PDGF-BB Protein 520-BB
Recombinant Rat PDGF-BB Protein
By Anonymous on 08/25/2021
Application: Stem/Immune cell maintenance or differentiation

10 ng/ml PDGF were added to ipsc cell culture medium.

Stem/Immune cell maintenance or differentiation Recombinant Rat PDGF-BB Protein 520-BB