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Recombinant Human IL-28B/IFN-lambda 3 Protein

Catalog #: 5259-IL
27 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
5259-IL-025/CF

With Carrier

Catalog # Availability Size / Price Qty
5259-IL-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (27)
FAQs
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-28B/IFN-lambda 3 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The ED50 for this effect is 0.0800-0.800 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human IL-28B/IFN-lambda 3 protein
Arg30-Val200, with the variant of Lys74Arg and a C-terminal 6-His tag
Accession #
AAN28264
N-terminal Sequence
Analysis
Arg30
Predicted Molecular Mass
20.1 kDa
SDS-PAGE
22 kDa, reducing conditions

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5259-IL (with carrier)

Product Datasheet
COA
SDS

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5259-IL/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5259-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5259-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and EDTA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-28B/IFN-lambda 3

IL-28B (also named interferon-lambda 3, IFN-lambda 3), IL-28A (IFN-lambda 2) and IL-29 (IFN-lambda 1) are type III interferons that are class II cytokine receptor ligands (1-4). They are distantly related to members of the IL-10 family and type I IFN family (1- 4). Human IL-28B cDNA encodes a 200 amino acid (aa) protein with a 25 aa signal peptide and a 175 aa mature protein that lacks N-glycosylation sites. Mature human IL-28B shares 64% and 75% aa sequence identity with mouse and canine IL-28B, respectively, and is active across species (5). Human IL-28B shares 94% and 69% aa identity with human IL-28A and IL‑29, respectively (4). Type III interferons are widely expressed, but are mainly produced by antigen presenting cells in response to viruses and double-stranded RNA that interact with Toll-like receptors or RIG-1 family helicases (2-6). They signal through a widely expressed receptor that is a heterodimer of the IL-10 receptor beta (IL-10 R beta ) and IL-28 receptor alpha (IL-28 R alpha ; also called IFN-lambda R1) (2, 3, 7, 9). Interaction of either type I or type III IFNs with their receptors activates similar pathways, including JAK tyrosine kinase activation, STAT phosphorylation and formation of the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex (1-3). Both type I and III IFNs induce anti‑viral activity and up‑regulate MHC class I antigen expression (2-6). Cell lines responsive to type III IFNs are also responsive to type I IFNs, but in general, higher concentrations of type III IFNs are needed for similar in vitro responses (8). In vivo, however, type III IFNs enhance levels of IFN-gamma in serum, suggesting that the robust anti-viral activity of type III IFNs may stem in part from activation of the immune system (5, 7). Anti-proliferative and antitumor activity in vivo has also been shown for type III IFNs (9-11).

References
  1. Chen, Q. et al. (2006) Vitam. Horm. 74:207.
  2. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  3. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
  4. Bartlett, N.W. et al. (2005) J. Gen. Virol. 86:1589.
  5. Ank, N. et al. (2006) J. Virol. 80:4501.
  6. Onoguchi, K. et al. (2007) J. Biol. Chem. 282:7576.
  7. Siebler, J. et al. (2007) Gastroenterology 132:358.
  8. Meager, A. et al. (2005) Cytokine 31:109.
  9. Lasfar, A. et al. (2006) Cancer Res. 66:4468.
  10. Sato, A. et al. (2006) J. Immunol. 176:7686.
  11. Zitzmann, K. et al. (2006) Biochem. Biophys. Res. Commun. 344:1334.
Long Name
Interleukin 28B
Entrez Gene IDs
282617 (Human); 338374 (Mouse)
Alternate Names
interleukin-28B; IFN-lambda 3; IL28B; IL-28B; IL28C; interferon, lambda 3

Citations for Recombinant Human IL-28B/IFN-lambda 3 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. MEF2A suppresses stress responses that trigger DDX41-dependent IFN production
    Authors: Smith, JR;Dowling, JW;McFadden, MI;Karp, A;Schwerk, J;Woodward, JJ;Savan, R;Forero, A;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. An RNA-based system to study hepatitis B virus replication and evaluate antivirals
    Authors: Y Yu, WM Schneider, MA Kass, E Michailidi, A Acevedo, AL Pamplona M, J Bordignon, A Koenig, CM Livingston, H van Gijzel, Y Ni, PM Ambrose, CA Freije, M Zhang, C Zou, M Kabbani, C Quirk, C Jahan, X Wu, S Urban, S You, A Shlomai, YP de Jong, CM Rice
    Science Advances, 2023-04-12;9(15):eadg6265.
    Species: Human
    Sample Types: Whole Cell
    Applications: Bioassay
  3. Genome-Wide Gene Expression Analysis Reveals Unique Genes Signatures of Epithelial Reorganization in Primary Airway Epithelium Induced by Type-I, -II and -III Interferons
    Authors: A Erb, UM Zissler, M Oelsner, AM Chaker, CB Schmidt-We, CA Jakwerth
    Biosensors, 2022-10-26;12(11):.
    Species: Human
    Sample Types: Organoids
    Applications: Bioassay
  4. An endogenously activated antiviral state restricts SARS-CoV-2 infection in differentiated primary airway epithelial cells
    Authors: L Broadbent, CGG Bamford, G Lopez Camp, S Manzoor, D Courtney, A Ali, O Touzelet, C McCaughey, K Mills, UF Power
    PLoS ONE, 2022-04-18;17(4):e0266412.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Distinct molecular phenotypes involving several human diseases are induced by IFN-lambda3 and IFN-lambda4 in monocyte-derived macrophages
    Authors: M De, A Bhushan, WS Grubbe, S Roy, JL Mendoza, S Chinnaswam
    Genes and immunity, 2022-02-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Development of a New Reverse Genetics System for Ebola Virus
    Authors: T Gan, D Zhou, Y Huang, S Xiao, Z Ma, X Hu, Y Tong, H Yan, J Zhong
    mSphere, 2021-05-05;6(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Sarbecovirus ORF6 proteins hamper induction of interferon signaling
    Authors: I Kimura, Y Konno, K Uriu, K Hopfensper, D Sauter, S Nakagawa, K Sato
    Cell Reports, 2021-03-12;34(13):108916.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Human Intestinal Organoids Recapitulate Enteric Infections of Enterovirus and Coronavirus
    Authors: X Zhao, C Li, X Liu, MC Chiu, D Wang, Y Wei, H Chu, JP Cai, I Hau-Yee Ch, K Kak-Yuen W, J Fuk-Woo Ch, K Kai-Wang T, KY Yuen, J Zhou
    Stem Cell Reports, 2021-02-12;16(3):493-504.
    Species: Primate - C. aethiops
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Activation of Interferon-Stimulated Transcriptomes and ACE2 Isoforms in Human Airway Epithelium Is Curbed by Janus Kinase Inhibitors
    Authors: HK Lee, O Jung, L Hennighaus
    Research square, 2020-12-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  10. Monocytes differentiated into macrophages and dendritic cells in the presence of human IFN-&lambda3 or IFN-&lambda4 show distinct phenotypes
    Authors: M De, A Bhushan, S Chinnaswam
    J Leukoc Biol, 2020-11-17;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  11. Interferons and viruses induce a novel truncated ACE2 isoform and not the full-length SARS-CoV-2 receptor
    Authors: OO Onabajo, AR Banday, ML Stanifer, W Yan, A Obajemu, DM Santer, O Florez-Var, H Piontkivsk, JM Vargas, TJ Ring, C Kee, P Doldan, DL Tyrrell, JL Mendoza, S Boulant, L Prokunina-
    Nat Genet, 2020-10-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Interferon-&lambda Enhances the Differentiation of Naive B Cells into Plasmablasts via the mTORC1 Pathway
    Authors: M Syedbasha, F Bonfiglio, J Linnik, C Stuehler, D Wüthrich, A Egli
    Cell Rep, 2020-10-06;33(1):108211.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Interferons and viruses induce a novel primate-specific isoform dACE2 and not the SARS-CoV-2 receptor ACE2
    Authors: OO Onabajo, AR Banday, W Yan, A Obajemu, ML Stanifer, DM Santer, O Florez-Var, H Piontkivsk, J Vargas, C Kee, DLJ Tyrrell, JL Mendoza, S Boulant, L Prokunina-
    bioRxiv, 2020-07-20;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Differential expression of interferon-lambda receptor 1 splice variants determines the magnitude of the antiviral response induced by interferon-lambda 3 in human immune cells
    Authors: DM Santer, GES Minty, DP Golec, J Lu, J May, A Namdar, J Shah, S Elahi, D Proud, M Joyce, DL Tyrrell, M Houghton
    PLoS Pathog., 2020-04-30;16(4):e1008515.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Structure-based glycoengineering of interferon lambda 4 enhances its productivity and anti-viral potency
    Authors: JH Chung, SH Hong, N Seo, TS Kim, HJ An, P Lee, EC Shin, HM Kim
    Cytokine, 2019-08-31;125(0):154833.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Type III interferon signaling restricts enterovirus 71 infection of goblet cells
    Authors: C Good, AI Wells, CB Coyne
    Sci Adv, 2019-03-06;5(3):eaau4255.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Adenovirus infection of human enteroids reveals interferon sensitivity and preferential infection of goblet cells
    Authors: MK Holly, JG Smith
    J. Virol., 2018-04-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. IFN-?4 potently blocks IFN-? signalling by ISG15 and USP18 in hepatitis C virus infection
    Authors: PS Sung, SH Hong, JH Chung, S Kim, SH Park, HM Kim, SK Yoon, EC Shin
    Sci Rep, 2017-06-19;7(1):3821.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Hepatitis-C-virus-induced microRNAs dampen interferon-mediated antiviral signaling
    Nat. Med., 2016-11-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Immune Cell Profiling of IFN-? Response Shows pDCs Express Highest Level of IFN-?R1 and Are Directly Responsive Via the JAK-STAT Pathway
    J Interferon Cytokine Res, 2016-09-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Resistin reinforces interferon ?-3 to eliminate hepatitis C virus with fine-tuning from RETN single-nucleotide polymorphisms
    Sci Rep, 2016-08-01;6(0):30799.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Impact and regulation of lambda interferon response in human metapneumovirus infection.
    Authors: Banos-Lara M, Harvey L, Mendoza A, Simms D, Chouljenko V, Wakamatsu N, Kousoulas K, Guerrero-Plata A
    J Virol, 2014-10-29;89(1):730-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Respiratory syncytial virus can infect basal cells and alter human airway epithelial differentiation.
    Authors: Persson B, Jaffe A, Fearns R, Danahay H
    PLoS ONE, 2014-07-17;9(7):e102368.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Epigenetic reprogramming of the type III interferon response potentiates antiviral activity and suppresses tumor growth.
    Authors: Ding S, Khoury-Hanold W, Iwasaki A, Robek M
    PLoS Biol, 2014-01-07;12(1):e1001758.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Hepatitis C virus pathogen associated molecular pattern (PAMP) triggers production of lambda-interferons by human plasmacytoid dendritic cells.
    Authors: Stone, Amy E L, Giugliano, Silvia, Schnell, Gretja, Cheng, Linling, Leahy, Katelyn, Golden-Mason, Lucy, Gale, Michael, Rosen, Hugo R
    PLoS Pathog, 2013-04-18;9(4):e1003316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. IFN-lambda3 inhibits HIV infection of macrophages through the JAK-STAT pathway.
    Authors: Liu MQ, Zhou DJ, Wang X, Zhou W, Ye L, Li JL, Wang YZ, Ho WZ
    PLoS ONE, 2012-04-27;7(4):e35902.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human IL-28B/IFN-lambda 3 Protein
By Jordan Gaston on 12/01/2017
Application: In vitro bioactivity in cell culture