Recombinant Rat GFR alpha-1 Fc Chimera Protein
Recombinant Rat GFR alpha-1 Fc Chimera Protein Summary
Product Specifications
| Rat GFR alpha -1 (Asp25-Leu445) Accession # Q62997 |
CSIEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
560-GR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
560-GR/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: GFR alpha-1/GDNF R alpha-1
Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF‑ beta superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha - (GFR alpha -1) or GFR alpha -2). GFR alpha -1 and -2 are members of a family of at least four cysteine‑rich glycosyl-phosphatidylinositol (GPI)‑linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase.
Rat GFR alpha -1 cDNA encodes a 468 amino acid (aa) residue protein with an N-terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% amino acid identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.
- Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
- Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
- Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
Citations for Recombinant Rat GFR alpha-1 Fc Chimera Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Postnatal development of mouse spermatogonial stem cells as determined by immunophenotype, regenerative capacity, and long-term culture-initiating ability: a model for practical applications
Authors: Song, Y;Zhang, X;Desmarais, JA;Nagano, M;
Scientific reports
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Dissecting the spermatogonial stem cell niche using spatial transcriptomics
Authors: Rajachandran, S;Zhang, X;Cao, Q;Caldeira-Brant, AL;Zhang, X;Song, Y;Evans, M;Bukulmez, O;Grow, EJ;Nagano, M;Orwig, KE;Chen, H;
Cell reports
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Constitutive activation of CTNNB1 results in a loss of spermatogonial stem cell activity in mice
Authors: A Boyer, X Zhang, A Levasseur, N Abou Nader, G St-Jean, MC Nagano, D Boerboom
PLoS ONE, 2021-05-20;16(5):e0251911.
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Gfra1 under-expression causes Hirschsprung's disease and associated enterocolitis in mice
Authors: LL Porokuokka, HT Virtanen, J Lindén, Y Sidorova, T Danilova, M Lindahl, M Saarma, JO Andressoo
Cell Mol Gastroenterol Hepatol, 2018-12-27;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
TGF-?1 promotes expression of fibrosis-related genes through the induction of histone variant H3.3 and histone chaperone HIRA
Authors: T Shindo, S Doi, A Nakashima, K Sasaki, K Arihiro, T Masaki
Sci Rep, 2018-09-19;8(1):14060.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
DDX4-EGFP transgenic rat model for the study of germline development and spermatogenesis
Authors: K Gassei, Y Sheng, A Fayomi, P Mital, M Sukhwani, CC Lin, KA Peters, A Althouse, H Valli, KE Orwig
Biol. Reprod., 2017-03-01;96(3):707-719.
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture -
Curcumin inhibits glial scar formation by suppressing astrocyte-induced inflammation and fibrosis in vitro and in vivo
Authors: Jiangkai Lin
Brain Res., 2016-11-16;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence
Nature, 2016-07-13;535(7612):440-3.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function.
Authors: Fonseca-Pereira D, Arroz-Madeira S, Rodrigues-Campos M, Barbosa I, Domingues R, Bento T, Almeida A, Ribeiro H, Potocnik A, Enomoto H, Veiga-Fernandes H
Nature, 2014-07-27;514(7520):98-101.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Substrate specificity of R3 receptor-like protein-tyrosine phosphatase subfamily toward receptor protein-tyrosine kinases.
Authors: Sakuraba, Juichi, Shintani, Takafumi, Tani, Sachiko, Noda, Masaharu
J Biol Chem, 2013-06-28;288(32):23421-31.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM).
Authors: Euteneuer S, Yang K, Chavez E, Leichtle A, Loers G, Olshansky A, Pak K, Schachner M, Ryan A
Mol Cell Neurosci, 2012-12-20;54(0):30-43.
Species: Rat
Sample Types: Whole Tissue
Applications: Bioassay -
Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
Authors: Kubota H, Wu X, Goodyear SM, Avarbock MR, Brinster RL
FASEB J., 2011-04-27;25(0):2604-14.
Species: Rabbit
Sample Types: Whole Cells
Applications: Bioassay -
Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin.
Authors: Carmillo P, Dago L, Day ES, Worley DS, Rossomando A, Walus L, Orozco O, Buckley C, Miller S, Tse A, Cate RL, Rosenblad C, Sah DW, Gronborg M, Whitty A
Biochemistry, 2005-02-22;44(7):2545-54.
Species: Rat
Sample Types: Recombinant Protein, Whole Cells
Applications: Bioassay, ELISA Developmet, Surface Plasmon Resonance -
The neuronal scaffold protein Shank3 mediates signaling and biological function of the receptor tyrosine kinase Ret in epithelial cells.
Authors: Schuetz G, Rosario M, Grimm J, Boeckers TM, Gundelfinger ED, Birchmeier W
J. Cell Biol., 2004-11-29;167(5):945-52.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The soluble ectodomain of RetC634Y inhibits both the wild-type and the constitutively active Ret.
Authors: Cerchia L, Libri D, Carlomagno MS, de Franciscis V
Biochem. J., 2003-06-15;372(0):897-903.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
TGFbeta induces GDNF responsiveness in neurons by recruitment of GFRalpha1 to the plasma membrane.
Authors: Peterziel H, Unsicker K, Krieglstein K
J. Cell Biol., 2002-10-07;159(1):157-67.
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay
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Average Rating: 4.5 (Based on 2 Reviews)
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Reason for Rating: Works well for our purposes (growing spermatogonial stem cells)
