Recombinant Human Neurexin 2 alpha Isoform 2 Fc Chimera, CF
Recombinant Human Neurexin 2 alpha Isoform 2 Fc Chimera, CF Summary
Product Specifications
When Recombinant Human Neurexin 2 alpha /NRXN2a Fc Chimera is coated at 2 μg/mL, biotinylated recombinant rat NXPH-1 binds with an apparent KD <10 nM.
| Human Neurexin 2 alpha (Met1 - Thr1566) Accession # NP_620060 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6636-NX
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Neurexin 2 alpha/NRXN2a
Neurexin 2, also known as NRXN2, is a neuronal type I transmembrane molecule that is encoded by one of three Neurexin genes (1, 2). Mature human Neurexin 2 alpha is an approximately 200 kDa N- and O‑glycosylated protein with a 1608 amino acid (aa) extracellular domain (ECD) and a 55 aa cytoplasmic domain. The ECD contains six LNS domains interspersed with three EGF-like domains and a juxtamembrane O‑glycosylation region (1 ‑ 3). The ECD of human Neurexin 2 alpha shares 98% and 99% aa sequence identity with mouse and rat Neurexin 2 alpha, respectively. Transcription of the Neurexin 2 gene from an internal promoter gives rise to Neurexin 2 beta which lacks the first five LNS domains and all of the EGF-like domains (3, 4). Human Neurexin 2 alpha is combinatorially spliced at five canonical sites in the ECD, giving rise to nearly 200 potential isoforms (4). This recombinant protein (isoform 2 according to SwissProt # Q9P2S2) does not contain inserts at splice sites 1, 2, 3,or 4. Neurexin 2 alpha is primarily expressed in the brain where it is localized to presynaptic terminals (3, 5, 6). It binds Neurexophilin 1 and 3, Neuroligin 2, and Dystroglycan (7 ‑ 9). Only Neurexin 2 isoforms that lack the insert at splice site 4 additionally bind LRRTM2 (10). The interaction between Neurexins and Neuroligins is complex and is restricted to particular combinations of splice forms of each binding partner (11). These interactions promote the differentiation and maintenance of postsynaptic GABA and NMDA but not AMPA terminals (8, 12). At presynaptic terminals as well as in pituitary melanotrophs, alpha Neurexins are required for coupling voltage-dependent calcium channel signaling to neurotransmitter or hormone release (13 ‑ 15).
- Craig, A.M. and Y. Kang (2007) Curr. Opin. Neurobiol. 17:43.
- Lise, M.-F. and A. El-Husseini (2006) Cell. Mol. Life Sci. 63:1833.
- Ushkaryov, Y.A. et al. (1992) Science 257:50.
- Rowen, L. et al. (2002) Genomics 79:587.
- Dean, C. et al. (2003) Nat. Neurosci. 7:708.
- Ullrich, B. et al. (1995) Neuron 14:497.
- Missler, M. et al. (1998) J. Biol. Chem. 273:34716.
- Kang, Y. et al. (2008) J. Biol. Chem. 283:2323.
- Sugita, S. et al. (2001) J. Cell Biol. 154:435.
- Siddiqui, T.J. et al. (2010) J. Neurosci. 30:7495.
- Boucard, A.A. et al. (2005) Neuron 48:229.
- Kattenstroth, G. et al. (2004) Proc. Natl. Acad. Sci. 101:2607.
- Missler, M. et al. (2003) Nature 423:939.
- Zhang, W. et al. (2005) J. Neurosci. 25:4330.
- Dudanova, I. et al. (2006) J. Neurosci. 26:10599.
Citation for Recombinant Human Neurexin 2 alpha Isoform 2 Fc Chimera, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Interaction of A? Oligomers with Neurexin 2? and Neuroligin 1 Mediates Synapse Damage and Memory Loss in Mice
Authors: J Brito-More, MV Lourenco, MM Oliveira, JF Vital, FC Ribeiro, JH Ledo, LP Diniz, MH Magdesian, HM Melo, F Barros-Ara, JM de Souza, SV Alves-Leon, FC Gomes, JR Clarke, CP Figueiredo, FG De Felice, ST Ferreira
J. Biol. Chem, 2017-03-10;0(0):.
Species: Mouse
Sample Types: Protein
Applications: Bioassay
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