Recombinant Human GFR alpha-1 Fc Chimera Protein, CF
Recombinant Human GFR alpha-1 Fc Chimera Protein, CF Summary
Product Specifications
| Human GFR alpha -1 (Ala19-Lys429) Accession # NP_665736 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
714-GR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: GFR alpha-1/GDNF R alpha-1
Glial cell line-derived growth factor (GDNF), neurturin (NTN), artemin and persephin are distant members of the TGF-beta superfamily. They function as neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits [GDNF receptor alpha -1 (GFR alpha -1) or GFR alpha -2]. GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane‑associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase.
Human GFR alpha -1 cDNA encodes a 465 amino acid (aa) residue protein with an N-terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, human GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% aa identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.
- Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
- Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
- Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
Citations for Recombinant Human GFR alpha-1 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Bimodal regulation of axonal transport by the GDNF-RET signalling axis in healthy and diseased motor neurons
Authors: ER Rhymes, AP Tosolini, AD Fellows, W Mahy, NQ McDonald, G Schiavo
Cell Death & Disease, 2022-07-07;13(7):584.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Optogenetic delivery of trophic signals in a genetic model of Parkinson's disease
Authors: A Ingles-Pri, N Furthmann, SH Crossman, AM Tichy, N Hoyer, M Petersen, V Zheden, J Biebl, E Reichhart, A Gyoergy, DE Siekhaus, P Soba, KF Winklhofer, H Janovjak
PloS Genetics, 2021-04-15;17(4):e1009479.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
RET signaling in prostate cancer
Authors: K Ban, S Feng, L Shao, M Ittmann
Clin. Cancer Res., 2017-05-10;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
DDX4-EGFP transgenic rat model for the study of germline development and spermatogenesis
Authors: K Gassei, Y Sheng, A Fayomi, P Mital, M Sukhwani, CC Lin, KA Peters, A Althouse, H Valli, KE Orwig
Biol. Reprod., 2017;96(3):707-719.
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture -
Parkin cooperates with GDNF/RET signaling to prevent dopaminergic neuron degeneration.
Authors: Meka D, Muller-Rischart A, Nidadavolu P, Mohammadi B, Motori E, Ponna S, Aboutalebi H, Bassal M, Annamneedi A, Finckh B, Miesbauer M, Rotermund N, Lohr C, Tatzelt J, Winklhofer K, Kramer E
J Clin Invest, 2015-03-30;125(5):1873-85.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Ret rescues mitochondrial morphology and muscle degeneration of Drosophila Pink1 mutants.
Authors: Klein P, Muller-Rischart A, Motori E, Schonbauer C, Schnorrer F, Winklhofer K, Klein R
EMBO J, 2014-01-28;33(4):341-55.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling.
Authors: Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R
J Neurosci, 2013-03-20;33(12):5399-410.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
RET PLC-gamma phosphotyrosine binding domain regulates Ca2+ signaling and neocortical neuronal migration.
Authors: Lundgren TK, Nakahata K, Fritz N, Rebellato P, Zhang S, Uhlen P
PLoS ONE, 2012-02-15;7(2):e31258.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi.
Authors: Lu B, PereiraPerrin M
Infect. Immun., 2008-06-09;76(8):3530-8.
Species: Bacteria
Sample Types: Trypanosoma
Applications: PcIP -
Neurturin-GFRalpha2 signaling controls liver bud migration along the ductus venosus in the chick embryo.
Authors: Tatsumi N, Miki R, Katsu K, Yokouchi Y
Dev. Biol., 2007-04-03;307(1):14-28.
Species: Chicken
Sample Types: Whole Tissue
Applications: Bioassay -
A specific survival response in dopamine neurons at most risk in Parkinson's disease.
Authors: Murase S, McKay RD
J. Neurosci., 2006-09-20;26(38):9750-60.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin.
Authors: Carmillo P, Dago L, Day ES, Worley DS, Rossomando A, Walus L, Orozco O, Buckley C, Miller S, Tse A, Cate RL, Rosenblad C, Sah DW, Gronborg M, Whitty A
Biochemistry, 2005-02-22;44(7):2545-54.
Species: Rat
Sample Types: Recombinant Protein, Whole Cells
Applications: Bioassay, ELISA Developmet, Surface Plasmon Resonance
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human GFR alpha-1 Fc Chimera Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human GFR alpha-1 Fc Chimera Protein, CF and earn rewards!
Have you used Recombinant Human GFR alpha-1 Fc Chimera Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
