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Recombinant Mouse E-Cadherin Fc Chimera Protein, CF

Catalog #: 748-EC
8 Citations Datasheet / COA / SDS
Newer Version Available: 8875-EC
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Discontinued Product

748-EC has been discontinued and is replaced by 8875-EC.

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Citations (8)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse E-Cadherin Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. When 5 x 104 cells/well are added to Recombinant Mouse E-Cadherin Fc Chimera coated plates (1.5 µg/mL with 100 µL/well), >50% cells will adhere after 90 minutes at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse E-Cadherin protein
Mouse E-Cadherin
(Asp157-Val709)
Accession # P09803		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
P09803
N-terminal Sequence
Analysis
Asp157
Predicted Molecular Mass
88.2 kDa (monomer)
SDS-PAGE
120 kDa, reducing conditions

Product Datasheets

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748-EC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

748-EC

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate, NaCl and CaCl2.
Reconstitution Reconstitute at 100 μg/mL in sterile DPBS with Ca2+ and Mg2+.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: E-Cadherin

E-Cadherin/Cadherin-1, also known as Uvomorulin in the mouse and rat, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion (1). Mouse E-Cadherin shares 92% and 81% amino acid sequence identity with the rat and human proteins, respectively (2). It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. E-Cadherin has five extracellular EC domains that form homophilic cis-clusters between adjacent epithelial cells and trans-clusters within the same cell. E-Cadherin clusters are critical components of adherens junctions between epithelial cells and act in the formation and maintenance of the epithelial cell barrier (3, 4). The intracellular domain of E-Cadherin binds to the Catenin cytoskeletal complex, which includes p120 Catenin, beta-Catenin, alpha-Catenin, and Vinculin. E-Cadherin expression is critical for epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition and cell migration, and E-Cadherin loss contributes to cancer metastasis (5). The extracellular E-Cadherin N-terminal domain can be cleaved by several proteases and is released as a soluble factor that enhances cancer cell motility and EGF R‑dependent survival and proliferation (6).

References
  1. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  2. Nagafuchi, A. et al. (1987) Nature 329:341.
  3. Guillot, C. and T. Lecuit (2013) Science 340:1185.
  4. Tian, X. et al. (2011) J. Biomed. Biotechnol. 2011:567305.
  5. Stemmler, M.P. (2008) Mol. Biosyst. 4:835.
  6. David, J.M. and A.K. Rajasekaran (2012) Cancer Res. 72:2917.
Entrez Gene IDs
999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin

Citations for Recombinant Mouse E-Cadherin Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. DamC reveals principles of chromatin folding in vivo without crosslinking and ligation
    Authors: J Redolfi, Y Zhan, C Valdes-Que, M Kryzhanovs, I Guerreiro, V Iesmantavi, T Pollex, RS Grand, E Mulugeta, J Kind, G Tiana, SA Smallwood, W de Laat, L Giorgetti
    Nat. Struct. Mol. Biol., 2019-05-27;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Local Integrin Activation in Pancreatic ? Cells Targets Insulin Secretion to the Vasculature
    Authors: WJ Gan, OH Do, L Cottle, W Ma, E Kosobrodov, J Cooper-Whi, M Bilek, P Thorn
    Cell Rep, 2018-09-11;24(11):2819-2826.e3.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Network plasticity of pluripotency transcription factors in embryonic stem cells.
    Authors: Filipczyk A, Marr C, Hastreiter S, Feigelman J, Schwarzfischer M, Hoppe P, Loeffler D, Kokkaliaris K, Endele M, Schauberger B, Hilsenbeck O, Skylaki S, Hasenauer J, Anastassiadis K, Theis F, Schroeder T
    Nat Cell Biol, 2015-09-21;17(10):1235-46.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Chondroitin sulfate is indispensable for pluripotency and differentiation of mouse embryonic stem cells.
    Authors: Izumikawa, Tomomi, Sato, Ban, Kitagawa, Hiroshi
    Sci Rep, 2014-01-15;4(0):3701.
    Species: Mouse
    Sample Types: Protein
    Applications: Binding Assay
  5. Soluble E-cadherin: a critical oncogene modulating receptor tyrosine kinases, MAPK and PI3K/Akt/mTOR signaling.
    Authors: Brouxhon, S M, Kyrkanides, S, Teng, X, Athar, M, Ghazizadeh, S, Simon, M, O'Banion, M K, Ma, L
    Oncogene, 2013-01-14;33(2):225-35.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. The ectodomain shedding of E-cadherin by ADAM15 supports ErbB receptor activation.
    Authors: Najy AJ, Day KC, Day ML
    J. Biol. Chem., 2008-04-22;283(26):18393-401.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Early loss of E-cadherin from cell-cell contacts is involved in the onset of Anoikis in enterocytes.
    Authors: Fouquet S, Lugo-Martinez VH, Faussat AM, Renaud F, Cardot P, Chambaz J, Pincon-Raymond M, Thenet S
    J. Biol. Chem., 2004-08-02;279(41):43061-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  8. The alpha 1 beta 1 and alpha E beta 7 integrins define a subset of dendritic cells in peripheral lymph nodes with unique adhesive and antigen uptake properties.
    Authors: Pribila JT, Itano AA, Mueller KL, Shimizu Y
    J. Immunol., 2004-01-01;172(1):282-91.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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