Recombinant Human PANP Fc Chimera Protein, CF

Recombinant Human PANP Fc Chimera Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: PANP

PILR‑alpha associated neural protein (PANP) is an approximately 30 kDa type 1 transmembrane glycoprotein that contains a 147 amino acid (aa) extracellular domain (ECD) and an 83 aa cytoplasmic domain (1). Within the ECD, human PANP shares 93% aa sequence identity with mouse and rat PANP. Alternative splicing may generate an additional isoform with a 6 aa truncation at the C‑terminus. PANP is preferentially expressed in the central nervous system (1). It binds to PILR‑alpha, an inhibitory receptor that is expressed on dendritic cells, neutrophils, and macrophages (1, 2). PILR‑alpha additionally binds to CD99, NPDC‑1, CL‑P1/COLEC12, and glycoprotein B of Herpes simplex virus type 1 (3‑5). PILR‑alpha binding requires the presence of sialylated O‑linked carbohydrates on each of these ligands, including PANP (1, 3, 4, 6). The in cis association of PILR‑alpha with sialylated molecules on the neutrophil surface inhibits beta integrin activation and neutrophil extravasation during inflammatory responses (7).