Recombinant Mouse Gas6 (Full Length) Protein, CF Summary
Product Specifications
Ala46-Pro673, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8310-GS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Mouse Gas6 (Catalog # 8310-GS) down regulates the expression of Axl in DU145 human prostate carcinoma cells. The ED50 for this effect is 20-100 ng/mL.
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1 μg/lane of Recombinant Mouse Gas6 (Full Length, Catalog # 8310-GS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing R bands at 85.4 and 69.2 kDa and NR band at 64.4 kDa.
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Background: Gas6
Gas6 (Growth Arrest Specific 6) is a secreted 75 kDa multimodular protein that is up-regulated by a wide variety of cell types in response to growth arrest. It is expressed by endothelial cells, fibroblasts, neurons, smooth muscle cells, and platelets, and it plays a role in vascular, thrombotic, atherosclerotic, inflammatory, autoimmune, renal, and cancer pathologies (1, 2). Both Gas6 and the related Protein S contain an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and two C-terminal Laminin G-like domains. And like Protein S, Gas6 is dependent upon vitamin K for activity. Within the Gla, EGF-like, and Laminin G-like domains, mouse Gas6 shares 83%, 94%, and 41% aa sequence identity with the equivalent regions in human Gas6, rat Gas6, and mouse Protein S, respectively. Gas6 binds to and induces signaling through the receptor tyrosine kinase TAM subfamily, which includes Axl, Dtk/Tyro3, and Mer (3). Shed soluble forms of Axl and Mer retain the ability to bind Gas6 and function as decoy receptors (4, 5). Gas6 participates in tissue homeostasis by protecting cells from stress-induced apoptosis and promoting apoptotic cell phagocytosis. The affinity of the gamma -carboxylated Gla domain for phosphatidylserine contributes to the role of Gas6 in phagocytosis as well as the cellular entry of select viruses (6, 7). Gas6 can function as a pro-inflammatory molecule by promoting platelet activation (8, 9) and can also inhibit inflammatory cytokine production from monocytes, macrophages, and microglia (10). In addition, Gas6 induces the proliferation of cardiac fibroblasts, Schwann cells, vascular smooth muscle cells, and the differentiation of NK cell precursors (11-14). It also inhibits VEGF-induced angiogenesis (15) and can have either positive or negative effects on tumor cell proliferation and invasion (16, 17).
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- Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
- Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
- Sather, S. et al. (2007) Blood 109:1026.
- Ekman, C. et al. (2010) J. Thromb. Haemost. 8:838.
- Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.
- Morizono, K. et al. (2011) Cell Host Microbe 9:286.
- Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
- Robins, R.S. et al. (2012) Blood 121:692.
- Alciato, F. et al. (2010) J. Leukoc. Biol. 87:869.
- Stenhoff, J. et al. (2004) Biochem. Biophys. Res. Commun. 319:871.
- Li, R. et al. (1996) J. Neurosci. 16:2012.
- Nakano, T. et al. (1995) J. Biol. Chem. 270:5702.
- Caraux, A. et al. (2006) Nat. Immunol. 7:747.
- Gallicchio, M. et al. (2005) Blood 105:1970.
- Loges, S. et al. (2010) Blood 115:2264.
- Gustafsson, A. et al. (2009) PLoS ONE 4:e7575.
Citations for Recombinant Mouse Gas6 (Full Length) Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Stromal changes in the aged lung induce an emergence from melanoma dormancy
Authors: ME Fane, Y Chhabra, GM Alicea, DA Maranto, SM Douglass, MR Webster, VW Rebecca, GE Marino, F Almeida, BL Ecker, DJ Zabransky, L Hüser, T Beer, HY Tang, A Kossenkov, M Herlyn, DW Speicher, W Xu, X Xu, EM Jaffee, JA Aguirre-Gh, AT Weeraratna
Nature, 2022-06-01;606(7913):396-405.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay -
Corticosterone inhibits GAS6 to govern hair follicle stem-cell quiescence
Authors: S Choi, B Zhang, S Ma, M Gonzalez-C, D Stein, X Jin, ST Kim, YL Kang, A Besnard, A Rezza, L Grisanti, JD Buenrostro, M Rendl, M Nahrendorf, A Sahay, YC Hsu
Nature, 2021-03-31;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Growth Arrest-Specific Gene 6 Administration Ameliorates Sepsis-Induced Organ Damage in Mice and Reduces ROS Formation In Vitro
Authors: L Salmi, F Gavelli, F Patrucco, M Bellan, PP Sainaghi, GC Avanzi, LM Castello
Cells, 2021-03-09;10(3):.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay -
Growth Arrest-Specific 6 Enhances the Suppressive Function of CD4(+)CD25(+) Regulatory T Cells Mainly through Axl Receptor
Authors: GJ Zhao, JY Zheng, JL Bian, LW Chen, N Dong, Y Yu, GL Hong, A Chandoo, YM Yao, ZQ Lu
Mediators Inflamm, 2017-02-08;2017(0):6848430.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
FAQs
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Is the Recombinant Mouse Gas6 (Full Length), CF (R&D Systems catalog # 8310-GS) gamma-carboxylated?
R&D Systems does not generally test for the presence of post translational modifications and has not confirmed if this protein is gamma-carboxylated. However, this protein is expressed in mammalian cells (NS0-cells), therefore it is possible that post translational modifications like the Gamma-carboxyglutamate will have occurred. This protein is biologically active, contains the Gla-domain, and is cultured in the presence of vitamin K, so it is expected to have a gamma-carboxylated amino terminus.
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