Home / Gas6 / Recombinant Human Gas6 Protein, CF

Recombinant Human Gas6 Protein, CF

Catalog #: 885-GSB
38 Citations 6 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
885-GSB-050
885-GSB-500
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Product Details
Citations (38)
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Supplemental Products
Reviews (6)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Gas6 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. Mishra, A. et al. (2012) Mol. Cancer Res. 10:703. The ED50 for this effect is 25-150 ng/mL
Source
Mouse myeloma cell line, NS0-derived human Gas6 protein
Ala49-Ala678, with a C-terminal 6-His tag
Accession #
NP_000811
N-terminal Sequence
Analysis
Ala49
Predicted Molecular Mass
70.5 kDa
SDS-PAGE
70-85 kDa, reducing conditions

Product Datasheets

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885-GSB

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

885-GSB

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Citrate.
Reconstitution Reconstitute at 500 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Graph showing bioactivity of recombinant human GAS6 View Larger

Recombinant Human Gas6 885-GSB down regulates the expression of Axl in DU145 human prostate carcinoma cells. The ED50 for this effect is 25-150 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Gas6

Gas6 (Growth Arrest Specific 6) is a multimodular protein that is up-regulated by a wide variety of cell types in response to growth arrest (1). Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG) (2). Human Gas6 is a 75 kDa protein that shares 77%-79% aa sequence identity with mouse and rat Gas6, and 43% aa identity with human protein S (over the region expressed). Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the
EGF‑like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer (3-5). Human Gas6 interacts with both mouse and rat orthologs of these receptors (1). The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl (6). Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors (7, 8). Gas6 induces a variety of responses, including prevention of apoptosis (9), cell proliferation (10), platelet-mediated thrombosis (11), retinal epithelial cell phagocytosis of outer rod segments (12), inhibition of VEGF-induced endothelial cell chemotaxis (13), and the differentiation and expansion of NK cell precursors (14). The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells (15). Several of these effects have been shown to require gamma -carboxylation of the Gla domain (12, 16).

References
  1. Hafizi, S. and B. Dahlback (2006) FEBS J. 273:5231.
  2. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
  3. Stitt, T.N. et al. (1995) Cell 80:661.
  4. Ohashi, K. et al. (1995) J. Biol. Chem. 270:22681.
  5. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
  6. Goruppi, S. et al. (1997) FEBS Lett. 415:59.
  7. Sather, S. et al. (2007) Blood 109:1026.
  8. Budagian, V. et al. (2005) Mol. Cell. Biol. 25:9324.
  9. Shankar, S.L. et al. (2006) J. Neurosci. 26:5638.
  10. Yanagita, M. et al. (2002) J. Clin. Invest. 110:239.
  11. Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
  12. Hall, M.O. et al. (2002) Exp. Eye Res. 75:391.
  13. Gallicchio, M. et al. (2005) Blood 105:1970.
  14. Caraux, A. et al. (2006) Nat. Immunol. 7:747.
  15. Wu, Y. et al. (2005) J. Cell Sci. 118:539.
  16. Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.
Long Name
Growth-arrest-specific Protein 6
Entrez Gene IDs
2621 (Human); 14456 (Mouse)
Alternate Names
AXLLG; AXLLGAXL stimulatory factor; AXSFAXL receptor tyrosine kinase ligand; DKFZp666G247; FLJ34709; Gas6; GAS-6; growth arrest-specific 6; growth arrest-specific protein 6

Citations for Recombinant Human Gas6 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. Targeting Tyro3, Axl, and MerTK Receptor Tyrosine Kinases Significantly Sensitizes Triple-Negative Breast Cancer to CDK4/6 Inhibition
    Authors: Demirsoy, S;Tran, H;Liu, J;Li, Y;Yang, S;Aregawi, D;Glantz, MJ;Jacob, NK;Walter, V;Schell, TD;Olmez, I;
    Cancers
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Regulation of Mertk Surface Expression via ADAM17 and ?-Secretase Proteolytic Processing
    Authors: Lahey, KC;Varsanyi, C;Wang, Z;Aquib, A;Gadiyar, V;Rodrigues, AA;Pulica, R;Desind, S;Davra, V;Calianese, DC;Liu, D;Cho, JH;Kotenko, SV;De Lorenzo, MS;Birge, RB;
    International journal of molecular sciences
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Western Blot
  3. Gas6-Axl Signaling Induces SRF/MRTF-A Gene Transcription via MICAL2
    Authors: Lundquist, MR;Jaffrey, SR;
    Genes
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Mertk-expressing microglia influence oligodendrogenesis and myelin modelling in the CNS
    Authors: Nguyen, LT;Aprico, A;Nwoke, E;Walsh, AD;Blades, F;Avneri, R;Martin, E;Zalc, B;Kilpatrick, TJ;Binder, MD;
    Journal of neuroinflammation
    Species: Xenopus, Transgenic Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  5. CNK2 promotes cancer cell motility by mediating ARF6 activation downstream of AXL signalling
    Authors: Serwe, G;Kachaner, D;Gagnon, J;Plutoni, C;Lajoie, D;Duramé, E;Sahmi, M;Garrido, D;Lefrançois, M;Arseneault, G;Saba-El-Leil, MK;Meloche, S;Emery, G;Therrien, M;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Gas6 ameliorates intestinal mucosal immunosenescence to prevent the translocation of a gut pathobiont, Klebsiella pneumoniae, to the liver
    Authors: Tsugawa, H;Ohki, T;Tsubaki, S;Tanaka, R;Matsuzaki, J;Suzuki, H;Hozumi, K;
    PLoS pathogens
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. PRAME Is a Novel Target of Tumor-Intrinsic Gas6/Axl Activation and Promotes Cancer Cell Invasion in Hepatocellular Carcinoma
    Authors: Hedrich, V;Breitenecker, K;Ortmayr, G;Pupp, F;Huber, H;Chen, D;Sahoo, S;Jolly, MK;Mikulits, W;
    Cancers
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Targeting AXL Using the AVB-500 Soluble Receptor and through Genetic Knockdown Inhibits Bile Duct Cancer Growth and Metastasis
    Authors: J Kim, G Nam, YK Shin, N Vilaplana-, HC Jeung, EJ Moon, IJ Lee
    Cancers, 2023-03-21;15(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. AXL regulates neuregulin1 expression leading to cetuximab resistance in head and neck cancer
    Authors: M Iida, NK McDaniel, KL Kostecki, NB Welke, CA Kranjac, P Liu, C Longhurst, JY Bruce, S Hong, R Salgia, DL Wheeler
    BMC Cancer, 2022-04-23;22(1):447.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. High Glucose Impairs Expression and Activation of MerTK in ARPE-19 Cells
    Authors: A Puddu, S Ravera, I Panfoli, N Bertola, D Maggi
    International Journal of Molecular Sciences, 2022-01-20;23(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Corosolic Acid Attenuates the Invasiveness of Glioblastoma Cells by Promoting CHIP-Mediated AXL Degradation and Inhibiting GAS6/AXL/JAK Axis
    Authors: LW Sun, SH Kao, SF Yang, SW Jhang, YC Lin, CM Chen, YH Hsieh
    Cells, 2021-10-28;10(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Anti-Axl monoclonal antibodies attenuate the migration of MDA-MB-231 breast cancer cells
    Authors: H Chang, R An, X Li, X Lang, J Feng, M Lv
    Oncology Letters, 2021-08-27;22(5):749.
    Species: Human
    Sample Types: Protein, Whole Cells
    Applications: Bioassay, ELISA (Capture)
  13. Quantitative Tyrosine Phosphoproteome Profiling of AXL Receptor Tyrosine Kinase Signaling Network
    Authors: X Wu, L Wang, NA Pearson, S Renuse, R Cheng, Y Liang, DG Mun, AK Madugundu, Y Xu, PS Gill, A Pandey
    Cancers, 2021-08-23;13(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. ST3GAL1 is a target of the SOX2-GLI1 transcriptional complex and promotes melanoma metastasis through AXL
    Authors: S Pietrobono, G Anichini, C Sala, F Manetti, LL Almada, S Pepe, RM Carr, BD Paradise, JN Sarkaria, JI Davila, L Tofani, I Battisti, G Arrigoni, L Ying, C Zhang, H Li, A Meves, ME Fernandez-, B Stecca
    Nat Commun, 2020-11-17;11(1):5865.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. KPNB1-mediated nuclear translocation of PD-L1 promotes non-small cell lung cancer cell proliferation via the Gas6/MerTK signaling pathway
    Authors: W Du, J Zhu, Y Zeng, T Liu, Y Zhang, T Cai, Y Fu, W Zhang, R Zhang, Z Liu, JA Huang
    Cell Death Differ, 2020-11-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. CD14+CD16- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK
    Authors: J Xue, L Xu, H Zhu, M Bai, X Li, Z Zhao, H Zhong, G Cheng, X Li, F Hu, Y Su
    Arthritis Research & Therapy, 2020-09-21;22(1):221.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. AXL confers cell migration and invasion by hijacking a PEAK1-regulated focal adhesion protein network
    Authors: A Abu-Thurai, MA Goyette, J Boulais, C Delliaux, C Apcher, C Schott, R Chidiac, H Bagci, MP Thibault, D Davidson, M Ferron, A Veillette, RJ Daly, AC Gingras, JP Gratton, JF Côté
    Nat Commun, 2020-07-17;11(1):3586.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Standard
  18. Distinct synovial tissue macrophage subsets regulate inflammation and remission in rheumatoid arthritis
    Authors: S Alivernini, L MacDonald, A Elmesmari, S Finlay, B Tolusso, MR Gigante, L Petricca, C Di Mario, L Bui, S Perniola, M Attar, M Gessi, AL Fedele, S Chilaka, D Somma, SN Sansom, A Filer, C McSharry, NL Millar, K Kirschner, A Nerviani, MJ Lewis, C Pitzalis, AR Clark, G Ferracciol, I Udalova, CD Buckley, E Gremese, IB McInnes, TD Otto, M Kurowska-S
    Nat. Med., 2020-06-29;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Cabozantinib inhibits AXL- and MET-dependent cancer cell migration induced by growth-arrest-specific 6 and hepatocyte growth factor
    Authors: T Hara, A Kimura, T Miyazaki, H Tanaka, M Morimoto, K Nakai, J Soeda
    Biochem Biophys Rep, 2020-01-17;21(0):100726.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  20. A TAZ-AXL-ABL2 Feed-Forward Signaling Axis Promotes Lung Adenocarcinoma Brain Metastasis
    Authors: JP Hoj, B Mayro, AM Pendergast
    Cell Rep, 2019-12-10;29(11):3421-3434.e8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  21. Interaction between androgen receptor and coregulator SLIRP is regulated by Ack1 tyrosine kinase and androgen
    Authors: D De Silva, Z Zhang, Y Liu, JS Parker, C Xu, L Cai, GG Wang, HS Earp, YE Whang
    Sci Rep, 2019-12-09;9(1):18637.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  22. A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET
    Authors: A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí
    Cell Mol Gastroenterol Hepatol, 2019-11-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  23. A gain-of-functional screen identifies the Hippo pathway as a central mediator of receptor tyrosine kinases during tumorigenesis
    Authors: T Azad, K Nouri, HJ Janse van, SM Maritan, L Wu, Y Hao, T Montminy, J Yu, P Khanal, LM Mulligan, X Yang
    Oncogene, 2019-09-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Axl Promotes Zika Virus Entry and Modulates the Antiviral State of Human Sertoli Cells
    Authors: DP Strange, B Jiyarom, N Pourhabibi, X Xie, C Baker, H Sadri-Arde, PY Shi, S Verma
    MBio, 2019-07-16;10(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. AXL Is a Novel Predictive Factor and Therapeutic Target for Radioactive Iodine Refractory Thyroid Cancer
    Authors: F Collina, L La Sala, F Liotti, N Prevete, E La Mantia, MG Chiofalo, G Aquino, L Arenare, M Cantile, G Liguori, F Di Gennaro, L Pezzullo, NS Losito, G Vecchio, G Botti, RM Melillo, R Franco
    Cancers (Basel), 2019-06-07;11(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Engineered AXL(-ECD)-Fc variants that abolish the AXL/Gas6 interaction suppress tumor cell migration.
    Authors: Y Duan, B Hu, C Qiao, L Luo, X Li, J Wang, H Liu, T Zhou, B Shen, M Lv, J Feng
    Oncol Lett, 2019-04-15;17(6):5784-5792.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay, ELISA (Capture)
  27. p5RHH nanoparticle-mediated delivery of AXL siRNA inhibits metastasis of ovarian and uterine cancer cells in mouse xenografts
    Authors: KA Mills, JM Quinn, ST Roach, M Palisoul, M Nguyen, H Noia, L Guo, J Fazal, DG Mutch, SA Wickline, H Pan, KC Fuh
    Sci Rep, 2019-03-18;9(1):4762.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Inhibition of MERTK Promotes Suppression of Tumor Growth in BRAF Mutant and BRAF Wild-Type Melanoma
    Authors: L Sinik, KA Minson, JJ Tentler, J Carrico, SM Bagby, WA Robinson, R Kami, T Burstyn-Co, SG Eckhardt, X Wang, SV Frye, HS Earp, D DeRyckere, DK Graham
    Mol. Cancer Ther., 2018-11-27;18(2):278-288.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. AXL phosphorylates and up-regulates TNS2 and its implications in IRS-1-associated metabolism in cancer cells
    Authors: LC Cheng, YL Chen, AN Cheng, AY Lee, CY Cho, JS Huang, SE Chuang
    J. Biomed. Sci., 2018-11-12;25(1):80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Activation
  30. Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology
    Authors: K Fujita, X Chen, H Homma, K Tagawa, M Amano, A Saito, S Imoto, H Akatsu, Y Hashizume, K Kaibuchi, S Miyano, H Okazawa
    Nat Commun, 2018-01-30;9(1):433.
    Applications: Bioassay
  31. Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma
    Authors: C Dantas-Bar, T Lesluyes, FL Loarer, F Chibon, I Treilleux, JM Coindre, P Meeus, M Brahmi, O Bally, I Ray-Coquar, MP Sunyach, AL Cesne, O Mir, S Bonvalot, M Toulmonde, A Italiano, P Saintigny, M Jean-Denis, F Ducimetier, D Ranchere, H El Sayadi, L Alberti, JY Blay
    Br. J. Cancer, 2017-10-12;117(12):1787-1797.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation
    Authors: SN Cross, JA Potter, P Aldo, JY Kwon, M Pitruzzell, M Tong, S Guller, CV Rothlin, G Mor, VM Abrahams
    J. Immunol., 2017-09-15;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  33. Inhibition of the Receptor Tyrosine Kinase AXL Restores Paclitaxel Chemosensitivity in Uterine Serous Cancer
    Authors: ML Palisoul, JM Quinn, E Schepers, IS Hagemann, L Guo, K Reger, AR Hagemann, CK McCourt, PH Thaker, MA Powell, DG Mutch, KC Fuh
    Mol. Cancer Ther., 2017-09-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. AXL receptor signalling suppresses p53 in melanoma through stabilization of the MDMX-MDM2 complex
    Authors: Zhi-Min Yuan
    J Mol Cell Biol, 2017-04-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. BCL6 promotes glioma and serves as a therapeutic target
    Authors: L Xu, Y Chen, M Dutra-Clar, A Mayakonda, M Hazawa, SE Savinoff, N Doan, JW Said, WH Yong, A Watkins, H Yang, LW Ding, YY Jiang, JW Tyner, J Ching, JP Kovalik, V Madan, SL Chan, M Müschen, JJ Breunig, DC Lin, HP Koeffler
    Proc. Natl. Acad. Sci. U.S.A, 2017-03-29;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Phosphatidylserine Sensing by TAM Receptors Regulates AKT-Dependent Chemoresistance and PD-L1 Expression.
    Authors: Kasikara C, Kumar S, Kimani S, Tsou W, Geng K, Davra V, Sriram G, Devoe C, Nguyen K, Antes A, Krantz A, Rymarczyk G, Wilczynski A, Empig C, Freimark B, Gray M, Schlunegger K, Hutchins J, Kotenko S, Birge R
    Mol Cancer Res, 2017-02-09;15(6):753-764.
    Applications: ELISA (Standard)
  37. Therapeutic activity of anti-AXL antibody against triple-negative breast cancer Patient Derived Xenografts and metastasis
    Authors: Wilhem Leconet
    Clin. Cancer Res, 2016-12-06;0(0):.
    Applications: Western Blot
  38. The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles
    Authors: KE Happonen, S Tran, M Mörgelin, R Prince, S Calzavarin, A Angelillo-, B Dahlbäck
    J. Biol. Chem., 2016-03-22;291(20):10586-601.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. Are the NS0 cells used to raise Recombinant Human Gas6 Protein, CF (Catalog # 885-GSB) cultured in the presence of Vitamin K?

    • Yes, the NS0 cells are cultured in the presence of Vitamin K.

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Reviews for Recombinant Human Gas6 Protein, CF

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Recombinant Human Gas6 Protein, CF
By Anonymous on 08/19/2022
Application: SDS-PAGE Control
Reason for Rating: Confirmed purity via reduced PAGE.
SDS-PAGE Control Recombinant Human Gas6 Protein, CF 885-GSB
Recombinant Human Gas6 Protein, CF
By Anonymous on 06/30/2021
Application: Immunoassay Standard
Immunoassay Standard Recombinant Human Gas6 Protein, CF 885-GSB
Recombinant Human Gas6 Protein, CF
By Anonymous on 01/04/2019
Application: In vitro bioactivity in cell culture
Recombinant Human Gas6 Protein, CF
By Ruchi Gupta on 07/09/2018
Application: SDS-PAGE Control
SDS-PAGE Control Recombinant Human Gas6 Protein, CF 885-GSB
Recombinant Human Gas6 Protein, CF
By Anonymous on 04/03/2018
Application: Binding assay/Protein-protein interaction
Recombinant Human Gas6 Protein, CF
By Anonymous on 04/26/2017