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Recombinant Human DPPIV/CD26 (High Purity Dimer) Protein, CF

Catalog #: 9168-SE
10 Citations Datasheet / COA / SDS
Newer Version Available: 11244-SE
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Discontinued Product

9168-SE has been discontinued and is replaced by 11244-SE.

Recombinant Human DPPIV/CD26 (High Purity Dimer) Protein Enzyme Activity
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Citations (10)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human DPPIV/CD26 (High Purity Dimer) Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >8500 pmol/min/µg as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human DPPIV/CD26 protein
Asn29-Pro766, with a C-terminal Asp-Ile and 6-His tag
Accession #
CAA43118
N-terminal Sequence
Analysis
Asn29
Structure / Form
Noncovalently-linked homodimer
Predicted Molecular Mass
86 kDa
SDS-PAGE
95-110 kDa, reducing condtions

Product Datasheets

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9168-SE

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9168-SE

Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Assay Procedure

Materials
  • Assay Buffer: 25 mM Tris, pH 8.0
  • Recombinant Human DPPIV/CD26 (rhDPPIV) (Catalog # 9168-SE)
  • Substrate: H-Gly-Pro-AMC (Bachem, Catalog # I-1225), 10 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhDPPIV to 0.1 ng/μL in Assay Buffer.
  2. Dilute Substrate to 200 μM in Assay Buffer.
  3. Load into a black plate 50 μL of 0.1 ng/μL rhDPPIV, and start the reaction by adding 50 μL of 200 μM Substrate. As a Substrate Blank combine 50 μL of Assay Buffer and 50 μL of 200 μM Substrate.
  4. Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes.
  5. Calculate specific activity:

 

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank.
     **Derived using calibration standard 7-amino, 4-Methyl Coumarin (Sigma, Catalog # A9891).

Per Well:
  • rhDPPIV: 0.005 μg
  • Substrate: 100 μM






















Scientific Data

Enzyme Activity Recombinant Human DPPIV/CD26 (High Purity Dimer) Protein Enzyme Activity View Larger

Recombinant Human DPPIV/CD26 (Catalog # 9168-SE) is measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The activity (orange) is approximately 4-fold greater than the competitor's DPPIV/CD26 (green).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DPPIV/CD26

DPPIV/CD26 (EC 3.4.14.5) is an approximately 110 kDa serine exopeptidase that releases Xaa-Pro or Xaa-Ala dipeptides from the N-terminus of oligo- and polypeptides. It regulates immune and endocrine function through the cleavage of multiple chemokines, growth factors, and peptide hormones (1, 2). Mature human DPPIV consists of a 6 amino acid (aa) cytoplasmic tail, a 22 aa transmembrane segment, and a 738 aa extracellular domain (ECD) that contains the catalytic active site (Ser, Asp, and His charge relay system) (3). Within the ECD, human DPPIV/CD26 shares 84% amino acid sequence identity with mouse and rat DPPIV. DPPIV is expressed as a noncovalent homodimer on the surface of epithelial cells, endothelial cells, and activated lymphocytes, and it can be released by MMP mediated shedding (4). It cleaves a range of peptide hormones including Glucagon, Glucagon-like Peptides 1 and 2, GIP, GHRH, Procalcitonin, Neuropeptide Y, and Substance P (5). It is released from adipocytes and induces insulin resistance in adipocytes and skeletal muscle (6). DPPIV also cleaves many chemokines, resulting in reduced chemotactic activity of CXCL6, 9, 10, 11, 12, and CCL5 (7-10) but unchanged angiostatic activity of CXCL9 and CXCL10 (8). Cleavage can increase (CCL5), decrease (CXCL12), or have no effect (CCL4) on chemokine blockade of HIV-1 cellular infectivity (7, 9, 11). In addition, DPPIV cleavage of CCL4 broadens chemokine receptor usage to also include CCR2b (11). DPPIV serves as a cell entry coreceptor for HIV and coronavirus (12, 13). It cleaves human GM-CSF and IL-3 and reduces their ability to promote myeloid cell development (14). It also interferes with CXCL12 induced hematopoietic cell migration, homing, and engraftment (15). DPPIV interacts in cis with adenosine deaminase on T cells and in trans with caveolin-1 on antigen presenting cells (16, 17). It provides costimulatory proliferation and activation signals to both CD4+ and CD8+ T cells (17, 18).

References
  1. Klemann, C. et al. (2016) Clin. Exp. Immunol. Epub PMID 26919392.
  2. Mortier, A. et al. (2016) J. Leukoc. Biol. Epub PMID 26744452.
  3. Tanaka, T. et al. (1992) J. Immunol. 149:481.
  4. Rohrborn, D. et al. (2014) FEBS Lett. 588:3870.
  5. Waumans, Y. et al. (2015) Front. Immunol. 6:387.
  6. Lamers, D. et al. (2011) Diabetes 60:1917.
  7. Proost, P. et al. (1998) J. Biol. Chem. 273:7222.
  8. Proost, P. et al. (2001) Blood 98:3554.
  9. Ohtsuki, T. et al. (1998) FEBS Lett. 431:236.
  10. Barreira da Silva, R. et al. (2015) Nat. Immunol. 16:850.
  11. Guan, E. et al. (2002) J. Biol. Chem. 277:32348.
  12. Callebaut, C. et al. (1993) Science 262:2045.
  13. Raj, V.S. et al. (2013) Nature 495:251.
  14. Broxmeyer, H.E. et al. (2012) Nat. Med. 18:1786.
  15. Christopherson II, K.W. et al. (2004) Science 305:1000.
  16. Kameoka, J. et al. (1993) Science 261:466.
  17. Ohnuma, K. et al. (2007) J. Biol. Chem. 282:10117.
  18. Hatano, R. et al. (2013) Immunology 138:165.
Long Name
Dipeptidyl-peptidase IV
Entrez Gene IDs
1803 (Human); 13482 (Mouse); 397492 (Porcine); 102133935 (Cynomolgus Monkey)
Alternate Names
ADABP; ADCP-2; ADCP2DPP IV; Adenosine deaminase complexing protein 2TP103; CD26 antigen; CD26; CD26T-cell activation antigen CD26; dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidyl-peptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP4; DPPIV; EC 3.4.14.5

Citations for Recombinant Human DPPIV/CD26 (High Purity Dimer) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. SDF-1 inhibits the dedifferentiation of islet beta cells in hyperglycaemia by up-regulating FoxO1 via binding to CXCR4
    Authors: XY Chen, YX Shi, YP Huang, M Ding, QL Shen, CJ Li, JN Lin
    Journal of Cellular and Molecular Medicine, 2021-12-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Dipeptidyl peptidase 4 contributes to Alzheimer's disease-like defects in a mouse model and is increased in sporadic Alzheimer's disease brains
    Authors: A Valverde, J Dunys, T Lorivel, D Debayle, AS Gay, C Caillava, M Chami, F Checler
    The Journal of Biological Chemistry, 2021-07-12;297(2):100963.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Continuous stimulation of dual-function peptide PGLP-1-VP inhibits the morbidity and mortality of NOD mice through anti-inflammation and immunoregulation
    Authors: H Gao, Q Zhao, S Tang, K Li, F Qin, Z Song, Y Pan, L Jin, Y Zhang
    Scientific Reports, 2021-02-11;11(1):3593.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Discovery of a novel fibroblast activation protein (FAP) inhibitor, BR103354, with anti-diabetic and anti-steatotic effects
    Authors: JM Cho, EH Yang, W Quan, EH Nam, HG Cheon
    Scientific Reports, 2020-12-04;10(1):21280.
    Species: N/A
    Sample Types:
    Applications: Bioassay
  5. Dipeptidyl Peptidase 4 Inhibitors Reduce Hepatocellular Carcinoma by Activating Lymphocyte Chemotaxis in Mice
    Authors: S Nishina, A Yamauchi, T Kawaguchi, K Kaku, M Goto, K Sasaki, Y Hara, Y Tomiyama, F Kuribayash, T Torimura, K Hino
    Cell Mol Gastroenterol Hepatol, 2018-09-11;7(1):115-134.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Pharmacological Effects of EGLP-1, a Novel Analog of Glucagon-Like Peptide-1, on Carbohydrate and Lipid Metabolism
    Authors: H Gao, Z Song, Q Zhao, Y Wu, S Tang, M Alahdal, Y Shen, Y Xing, Y Pan, J Li, Y Zhang, L Jin
    Cell. Physiol. Biochem., 2018-07-24;48(3):1112-1122.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Reduction of soluble dipeptidyl peptidase 4 levels in plasma of patients infected with Middle East respiratory syndrome coronavirus
    Authors: KS Inn, Y Kim, A Aigerim, U Park, ES Hwang, MS Choi, YS Kim, NH Cho
    Virology, 2018-03-26;518(0):324-327.
    Species: Virus
    Sample Types: Virus
    Applications: Bioassay
  8. Pericyte-targeting prodrug overcomes tumor resistance to vascular disrupting agents
    Authors: M Chen, X Lei, C Shi, M Huang, X Li, B Wu, Z Li, W Han, B Du, J Hu, Q Nie, W Mai, N Ma, N Xu, X Zhang, C Fan, A Hong, M Xia, L Luo, A Ma, H Li, Q Yu, H Chen, D Zhang, W Ye
    J. Clin. Invest., 2017-08-28;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  9. A novel recombinant antibody specific to full-length stromal derived factor-1 for potential application in biomarker studies
    Authors: DI Bromage, S Taferner, M Pillai, DM Yellon, SM Davidson
    PLoS ONE, 2017-04-05;12(4):e0174447.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA (Standard)
  10. Improved glycaemia in high-fat-fed neprilysin-deficient mice is associated with reduced DPP-4 activity and increased active GLP-1 levels
    Authors: Joshua R Willard
    Diabetologia, 2016-12-08;0(0):.
    Applications: Bioassay

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