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Recombinant Cynomolgus DNAM-1/CD226 Fc Chimera Protein, CF

Catalog #: 9276-DN Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9276-DN-100
Recombinant Cynomolgus DNAM-1/CD226 Fc Chimera Protein Bioactivity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus DNAM-1/CD226 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey DNAM-1/CD226 Fc Chimera is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) that produces 50% of the optimal binding response is approximately
0.2-1 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey DNAM-1/CD226 protein
Cynomolgus Monkey DNAM-1/CD226
(Glu19-Asn247)
Accession # XP_005586537		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
XP_005586537
N-terminal Sequence
Analysis
Glu19
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
53 kDa
SDS-PAGE
74-87 kDa, reducing conditions

Product Datasheets

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9276-DN

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9276-DN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Cynomolgus DNAM-1/CD226 Fc Chimera Protein Bioactivity View Larger

When Recombinant Cynomolgus Monkey DNAM-1/CD226 Fc Chimera (Catalog # 9276-DN) is immobilized at 0.25 µg/mL, 100 µL/well, Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) binds with a typical ED50 of 0.2-1 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature cynomolgus DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, cynomolgus DNAM-1 shares 93%, 53% and 50% aa sequence identity with human, mouse, and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid, myeloid cells, and activated vascular endothelial cells and interacts with CD155/PVR and Nectin-2/CD112 (3-5). It competes with CD96 and TIGIT for binding to these proteins (6). It associates in cis with Integrin beta 2/CD18 on activated, but not resting, NK, T, and mast cells (5, 7, 8). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (2-4, 7, 9), Th17 polarization (10), dendritic cell maturation, megakaryocyte, and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it also inhibits the formation of osteoclasts (11-14). Platelet-endothelium interactions mediated by DNAM-1 enable the metastasis of tumor cells to the lung (15). Downregulation of DNAM-1 on tumor cells can interfere with anti-tumor cellular immunity (16).

References
  1. Ceboni, C. et al. (2013) Front. Immunol. 4:508.
  2. Shibuya, A. et al. (1996) Immunity 4:573.
  3. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
  4. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
  5. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
  6. Chan, C.J. et al. (2014) Nat. Immunol. 15:431.
  7. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
  8. Shibuya, K. et al. (1999) Immunity 11:615.
  9. Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
  10. Lozano, E. et al. (2013) J. Immunol. 191:3673.
  11. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  12. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
  13. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
  14. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
  15. Morimoto, K. et al. (2007) Oncogene 27:264.
  16. Carlsten, M. et al. (2009) J. Immunol. 183:4921.
Long Name
DNAX Accessory Molecule 1
Entrez Gene IDs
10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)
Alternate Names
CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1

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