Recombinant Human BAI2 Protein Fc Chimera, CF

Catalog #: 9338-BA Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9338-BA-050

Recombinant Human BAI2 Protein Fc Chimera Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BAI2 Protein Fc Chimera, CF Summary

Product Specifications

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL3 (Catalog # 9115-TN) is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI2 Fc Chimera that produces 50% of the optimal binding response is approximately 25-150 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human BAI2 protein

Human BAI2 (Phe21-Asp911 ) Accession # O60241-3	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

O60241-3

N-terminal Sequence
Analysis

Phe21

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

123 kDa

SDS-PAGE

128-200 kDa, reducing conditions

Product Datasheets

9338-BA

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9338-BA

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Human C1qL3 (Catalog # 9115-TN) is coated at 2 µg/mL (100 µL/well), Recombinant Human BAI2 Fc Chimera (Catalog # 9338-BA) binds with an ED₅₀ of 25-150 ng/mL.

Reconstitution Calculator

Background: BAI2

Brain angiogenesis inhibitor 2 (BAI2), along with BAI1 and BAI3, form a subfamily of brain specific adhesion GPCRs. They are large, glycosylated molecules, approximately 180-220 kDa, and play a role in neuronal synapse formation and maintenance. Mature human BAI2 consists of a 903 amino acid (aa) N-terminal extracellular domain (ECD) containing four TSP1 domains, a hormone binding domain (HBD), a GAIN domain and a GPS protease sensitive linker, followed by a region with seven transmembrane segments and a 410 aa C-terminal cytoplasmic domain (1). Within the N-terminal ECD, human BAI2 shares 96% aa sequence identity with both mouse and rat BAI2. BAI2 is expressed primarily in neurons and astrocytes of the hippocampus and cerebral cortex in the brain (2, 3). To become functional, BAI2 might require activation by cleavage at the GPS domain (4). BAI family members are known to bind the C1q-like complement (C1qL) family via thrombospondin type 1 repeats (TSRs) which are conserved between BAI members (5). BAI2 has been shown to suppress expression of VEGF leading to increased neurogenesis in the dentate gyrus of the hippocampus (6).

References