Recombinant Human VCAM-1/CD106 Protein, CF

Catalog #: ADP5
9 Citations Datasheet / COA / SDS

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ADP5-050
ADP5-200

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Citations (9)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human VCAM-1/CD106 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10⁴ cells/well are added to human sVCAM-1 coated plates (10 μg/mL with 100 μL/well), >85% will adhere after 1 hour incubation at room temperature.

Source

Chinese Hamster Ovary cell line, CHO-derived human VCAM-1/CD106 protein
Phe25-Glu698 (Arg322Gly)

Accession #

P19320-1

Predicted Molecular Mass

74.1 kDa

SDS-PAGE

93-95 kDa, reducing conditions

Product Datasheets

ADP5

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

ADP5

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
Reconstitution	Reconstitute at 1 mg/mL in sterile, deionized water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: VCAM-1/CD106

Human VCAM-1 (CD106), a member of the Immunoglobulin Superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7.During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

Long Name

Vascular Cell Adhesion Molecule 1

Entrez Gene IDs

7412 (Human); 22329 (Mouse)

Alternate Names

CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1

Related Research Areas

Citations for Recombinant Human VCAM-1/CD106 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Cell adhesion to collagen promotes leukemia resistance to doxorubicin by reducing DNA damage through the inhibition of Rac1 activation
Authors: D Naci, S Berrazouan, F Barabé, F Aoudjit
Sci Rep, 2019-12-19;9(1):19455.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
The Synthetic Dipeptide Pidotimod Shows a Chemokine-Like Activity through CXC Chemokine Receptor 3 (CXCR3)
Authors: F Caccuri, A Bugatti, S Corbellini, S Roversi, A Zani, P Mazzuca, S Marsico, A Caruso, C Giagulli
Int J Mol Sci, 2019-10-24;20(21):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity
Sci Rep, 2016-11-28;6(0):37953.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Overexpression of progelatinase B/proMMP-9 affects migration regulatory pathways and impairs chronic lymphocytic leukemia cell homing to bone marrow and spleen.
Authors: Bailon E, Ugarte-Berzal E, Amigo-Jimenez I, Van den Steen P, Opdenakker G, Garcia-Marco J, Garcia-Pardo A
J Leukoc Biol, 2014-08-01;96(2):185-99.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
A novel CD44-binding peptide from the pro-matrix metalloproteinase-9 hemopexin domain impairs adhesion and migration of chronic lymphocytic leukemia (CLL) cells.
Authors: Ugarte-Berzal E, Bailon E, Amigo-Jimenez I, Albar J, Garcia-Marco J, Garcia-Pardo A
J Biol Chem, 2014-04-16;289(22):15340-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
Authors: Barkho BZ, Song H, Aimone JB
Stem Cells Dev., 2006-06-01;15(3):407-21.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
Urokinase-type plasminogen activator modulates airway eosinophil adhesion in asthma.
Authors: Brooks AM, Bates ME, Vrtis RF, Jarjour NN, Bertics PJ, Sedgwick JB
Am. J. Respir. Cell Mol. Biol., 2006-05-25;35(4):503-11.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1.
Authors: Yamamoto H, Nagata M, Sakamoto Y
Ann. Allergy Asthma Immunol., 2005-02-01;94(2):292-300.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Bioassay
Characterization of hematopoietic progenitor mobilization in protease-deficient mice.
Authors: Levesque JP, Liu F, Simmons PJ, Betsuyaku T, Senior RM, Pham C, Link DC
Blood, 2004-03-09;104(1):65-72.
Species: Mouse
Sample Types: Bone Marrow Fluid
Applications: Bioassay