Mouse IL-2 Antibody

Catalog #: AF-402-NA
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF-402-NA
AF-402-SP

Cell Proliferation Induced by IL‑2 and Neutralization by Mouse IL‑2 Antibody.

1 Image

All IL-2 Antibodies

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant rat IL‑2 and recombinant cotton rat IL‑2 is observed and less than 2% cross-reactivity with recombinant human IL‑2, recombinant canine IL‑2, recombinant feline IL‑2, recombinant equine IL‑2, recombinant bovine IL‑2, and recombinant porcine IL‑2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse IL-2 (R&D Systems, Catalog # 402-ML)
Ala21-Gln169
Accession # P04351

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑2 (Catalog # 402-ML)

ELISA

This antibody functions as an ELISA detection antibody when paired with Rabbit Anti-Mouse IL‑2 Monoclonal Antibody(Catalog # MAB4022).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse IL-2 DuoSet ELISA Kit (Catalog # DY402) for convenient development of a sandwich ELISA or the Mouse IL-2 Quantikine ELISA Kit (Catalog # M2000) for a complete optimized ELISA.

Neutralization

Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line [Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 276]. The Neutralization Dose (ND₅₀) is typically 0.1-0.3 µg/mL in the presence of 2 ng/mL Recombinant Mouse IL‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

View Larger

Cell Proliferation Induced by IL‑2 and Neutralization by Mouse IL‑2 Antibody. Recombinant Mouse IL‑2 (Catalog # 402-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-402-NA). The ND₅₀ is typically 0.1-0.3 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

Interleukin-2 (IL-2) is an O-glycosylated four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4⁺ and CD8⁺ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils. Mature mouse IL-2 shares 56% and 73% aa sequence identity with human and rat IL-2, respectively. It shows strain-specific heterogeneity in an N-terminal region that contains a poly-glutamine stretch. Mouse and human IL-2 exhibit cross-species activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis. It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4⁺ T cells but not activated CD4⁺ memory lymphocytes. IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells. Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity.

Long Name

Interleukin 2

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Alternate Names

Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IL-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

Enhanced production of IL-2 from anti-CD3 antibody-stimulated mouse spleen cells by artepillin C, a major component of Brazilian green propolis
Authors: H Tsuruta, M Mizuno-Kam, M Takahashi, M Ando, K Ikeno, K Ueno, E Takayama, H Kawaki, G Nakamura, T Nikaido, H Fujita, N Kondoh
Journal of oral biosciences, 2022-06-03;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Atherogenic effect of interleukin-2 and antiatherogenic effect of interleukin-2 antibody in apo-E-deficient mice.
Authors: Upadhya S, Mooteri S, Peckham N, Pai RG
Angiology, 2004-05-01;55(3):289-94.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization