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Human alpha -L-Iduronidase/IDUA Antibody

Catalog #: AF4119
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4119
AF4119-SP
Detection of Human alpha ‑L‑Iduronidase/IDUA by Western Blot.
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Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha -L-Iduronidase/IDUA Antibody Summary

Species Reactivity
Human
Specificity
Detects human alpha ‑L‑Iduronidase/IDUA in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human alpha ‑L‑Iduronidase/IDUA
Ala26-Pro653 (Ala26Thr)
Accession # AAA81589
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human alpha -L-Iduronidase/IDUA (Catalog # 4119-GH), see our available Western blot detection antibodies
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human a-L-Iduronidase/IDUA antibody by Western Blot. View Larger

Detection of Human alpha ‑L‑Iduronidase/IDUA by Western Blot. Western blot shows lysates of human kidney tissue and IMR-90 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human a-L-Iduronidase/IDUA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4119) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for a-L-Iduronidase/IDUA at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry a-L-Iduronidase/IDUA antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

alpha ‑L‑Iduronidase/IDUA in HepG2 Human Cell Line. a-L-Iduronidase/IDUA was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human a-L-Iduronidase/IDUA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4119) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-L-Iduronidase/IDUA

alpha -L-Iduronidase encoded by the IDUA gene is an important enzyme required for the lysosomal degradation of glycosaminoglycans (GAGs). It hydrolyzes the non‑reducing terminal alpha -L-iduronic acid residues in GAGs including dermatan sulfate and heparan sulfate. Mutations in IDUA that result in enzymatic deficiency lead to the autosomal recessive disease mucopolysaccharidosis type I (MPS I) (1). MPS I causes progressive cellular, tissue and organ damage, and several clinical studies using enzyme replacement therapy have shown promising benefits (2).

References
  1. Scott, H.S. et al. (1995) Hum. Mutat. 6:288.
  2. Wraith, J.E. (2005) Expert Opin. Pharmacother. 6:489.
Entrez Gene IDs
3425 (Human); 15932 (Mouse); 360904 (Rat); 102116889 (Cynomolgus Monkey)
Alternate Names
alphaLIduronidase; alpha-L-Iduronidase; IDA; IDUA; MPS1; MPSI

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Citations for Human alpha -L-Iduronidase/IDUA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. STEP� vectors for rapid generation of stable transfected CHO cell pools and clones with high expression levels and product quality homogeneity of difficult-to-express proteins
    Authors: A Luthra, RA Spanjaard, S Cheema, N Veith, L Kober, Y Wang, T Jing, Y Zhao, F Hoeksema, C Yallop, M Havenga, WAM Bakker
    Protein Expression and Purification, 2021-05-25;0(0):105920.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  2. ZFN-Mediated In�Vivo Genome Editing Corrects Murine Hurler Syndrome
    Authors: L Ou, RC DeKelver, M Rohde, S Tom, R Radeke, SJ St Martin, Y Santiago, S Sproul, MJ Przybilla, BL Koniar, KM Podetz-Ped, K Laoharawee, RD Cooksley, KE Meyer, MC Holmes, RS McIvor, T Wechsler, CB Whitley
    Mol. Ther., 2018-11-01;0(0):.
    Species: Transgenic Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Toxicology Study of Intra-Cisterna Magna Adeno-Associated Virus 9 Expressing Human Alpha-L-Iduronidase in Rhesus Macaques
    Authors: J Hordeaux, C Hinderer, T Goode, N Katz, EL Buza, P Bell, R Calcedo, LK Richman, JM Wilson
    Mol Ther Methods Clin Dev, 2018-07-14;10(0):79-88.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Mutation in VPS33A affects metabolism of glycosaminoglycans: a new type of mucopolysaccharidosis with severe systemic symptoms
    Authors: Hidehito Kondo
    Hum. Mol. Genet, 2017-01-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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