Human PSMA/FOLH1/NAALADase I Antibody Summary
Lys44-Ala750
Accession # Q04609
Applications
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human PSMA/FOLH1/NAALADase I Monoclonal Antibody (Catalog # MAB42341).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human PSMA/FOLH1 DuoSet ELISA Kit (Catalog # DY4234-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human PSMA/FOLH1/ NAALADase I by Western Blot. Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human PSMA/FOLH1/ NAALADase I Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF4234) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PSMA/FOLH1/NAALADase I at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of PSMA/FOLH1/ NAALADase I in LnCAP Human Cell Line by Flow Cytometry. LnCAP human prostate cancer cell line was stained with Human PSMA/FOLH1/NAALADase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4234, filled histo-gram) or control antibody (Catalog # 5-001-A, open histo-gram), followed by Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
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PSMA/FOLH1/NAALADase I in Human Prostate Cancer Tissue. PSMA/FOLH1/NAALADase I was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human PSMA/FOLH1/NAALADase I Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF4234) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific labeling was localized to the plasma membrane and extracellular space. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Human PSMA/FOLH1/NAALADase I ELISA Standard Curve. Recombinant Human PSMA/FOLH1/NAALADase I protein was serially diluted 2-fold and captured by Mouse Anti-Human PSMA/FOLH1/NAALADase I Monoclonal Antibody (MAB42341) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Human PSMA/FOLH1/NAALADase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4234) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PSMA/FOLH1/NAALADase I
Human prostate-specific membrane antigen (PSMA), a tumor marker in prostate cancer encoded by the FOLH1 gene, is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine (1, 2). The enzyme is also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1, folypoly-gamma-glutamate carboxypeptidase (FGCP), and N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I). In the brain, PSMA hydrolyzes the neurotransmitter N-acetyl-Asp-Glu to produce glutamate, another neurotransmitter. Inhibition of brain PSMA activity is considered to be a promising approach for the treatment of neurological disorders associated with glutamate excitotoxicity, such as stroke, chronic pain, and amyotrophic lateral sclerosis (3). Intestinal PSMA hydrolyzes folylpoly-gamma -glutamates, facilitating the uptake of folate (4).
- Silver, D.A. et al. (1997) Clin. Cancer Res. 3:81.
- Carter, R.E. et al. (1996) Pro. Natl. Acad. Sci. USA 93:749.
- Jackson, P.F. and B.S. Slusher (2001) Curr. Med. Chem. 8:949.
- Heston, W.D. (1997) Urology 49:104.
Product Datasheets
Citations for Human PSMA/FOLH1/NAALADase I Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Prostate cancer extracellular vesicle digital scoring assay - a rapid noninvasive approach for quantification of disease-relevant mRNAs
Authors: JJ Wang, N Sun, YT Lee, M Kim, T Vagner, K Rohena-Riv, Z Wang, Z Chen, RY Zhang, J Lee, C Zhang, H Tang, J Widjaja, TX Zhang, D Qi, PC Teng, YJ Jan, KC Hou, C Hamann, HM Sandler, TJ Daskivich, DJ Luthringer, NA Bhowmick, R Pei, S You, DD Vizio, HR Tseng, JF Chen, Y Zhu, EM Posadas
Nano Today, 2023-01-03;48(0):.
Species: Human
Sample Types: Plasma
Applications: Capture Assay -
Diagnostic potential of serum extracellular vesicles expressing prostate-specific membrane antigen in urologic malignancies
Authors: K Kawakami, Y Fujita, T Kato, K Horie, T Koie, K Umezawa, H Tsumoto, Y Miura, Y Katagiri, T Miyazaki, I Ohsawa, K Mizutani, M Ito
Scientific Reports, 2021-07-22;11(1):15000.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Capture
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