Summary
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Human IL-18 BPa Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)
Sensitivity
7.52 pg/mL
Assay Range
26.6 - 1,700 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human IL-18 BPa
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human IL-18 BPa Immunoassay is a 4.5 hour solid phase ELISA
designed to measure IL-18 BPa in cell culture supernates, cell lysates,
serum, and plasma. It contains NS0-expressed recombinant human IL-18 BPa
and antibodies raised against the recombinant factor. This immunoassay
has been shown to accurately quantitate recombinant human IL-18 BPa.
Results obtained using natural human IL-18 BPa showed linear curves that
were parallel to the standard curves obtained using the Quantikine kit
standards. These results indicate that this kit can be used to
determine relative mass values for natural human IL-18 BPa.
Recovery
The recovery of human IL-18 BPa spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 106 | 98-114 |
| EDTA Plasma (n=4) | 106 | 93-113 |
| Heparin Plasma (n=4) | 105 | 92-119 |
| Serum (n=4) | 102 | 92-113 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of human IL-18 BPa were serially diluted with the
appropriate Calibrator Diluent to produce samples with values within
the dynamic range of the assay.
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-18 BPa
Interleukin 18 binding proteins are secreted glycoproteins which antagonize IL-18 activity by binding IL-18 and blocking its biological activity. Alternative splicing of the IL-18 BP gene produces at least four isoforms that differ in affinity for IL-18. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig-like domain.
Long Name:
Interleukin 18 Binding Protein Isoform a
Entrez Gene IDs:
10068 (Human); 84388 (Rat); 107126419 (Cynomolgus Monkey)
Alternate Names:
IL18 BPa; IL-18 BPa; IL18BP; IL-18BP; IL18BPa; interleukin 18 binding protein; interleukin-18-binding protein; MC51L-53L-54L homolog gene product; tadekinig-alfa
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL of Conjugate to each well.
- For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
For Cell Culture Supernate/Urine Samples: Cover with a new plate sealer, and incubate at room temperature for 20 minutes. PROTECT FROM LIGHT. - Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Citations for Human IL-18 BPa Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
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Anti-Inflammatory and Anti-(Lymph)angiogenic Properties of an ABCB5+ Limbal Mesenchymal Stem Cell Population
Authors: Meshko, B;Volatier, TLA;Mann, J;Kluth, MA;Ganss, C;Frank, MH;Frank, NY;Ksander, BR;Cursiefen, C;Notara, M;
International journal of molecular sciences
Species: Human
Sample Types: Cell Culture Supernates
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Increased Levels of Caspase-1 and IL-1? Among Adults With Persistent Immune Activation After 12 Years of Suppressive Antiretroviral Therapy in the Infectious Diseases Institute HIV Treatment Cohort
Authors: Nabatanzi, R;Ssekamatte, P;Castelnuovo, B;Kambugu, A;Nakanjako, D;
Open forum infectious diseases
Species: Human
Sample Types: Plasma
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Role of NLRP3 inflammasome activation in HCC cell progression
Authors: Dai, B;Cao, H;Hu, Y;Gong, Z;Huang, X;Chen, Y;Liu, F;Peng, X;Zhang, Y;Lei, X;
Heliyon
Species: Human
Sample Types: Cell Culture Supernates
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Pharmacokinetic Study of rhIL-18BP and Its Effect on Radiation-Induced Cytokine Changes in Mouse Serum and Intestine
Authors: W Cui, L Hull, A Zizzo, L Wang, B Lin, M Zhai, M Xiao
Toxics, 2022-12-30;11(1):.
Species: Mouse
Sample Types: Serum
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Expression of Circulating MicroRNAs and Myokines and Interactions with Serum Osteopontin in Type 2 Diabetic Patients with Moderate and Poor Glycemic Control: A Biochemical and Molecular Study
Authors: HA Al-Rawaf, AH Alghadir, SA Gabr
BioMed Research International, 2021-12-07;2021(0):7453000.
Species: Human
Sample Types: Serum
-
The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males.
Authors: Tominaga T, Ikemura T, Yada K, Kanda K, Sugama K, Ma S, Choi W, Araya M, Huang J, Nakamura N, Suzuki K
Antioxidants (Basel), 2021-05-28;10(6):.
Species: Human
Sample Types: Urine
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A pilot study evaluating GSK1070806 inhibition of interleukin-18 in renal transplant delayed graft function
Authors: E Wlodek, RB Kirkpatric, S Andrews, R Noble, R Schroyer, J Scott, CJE Watson, M Clatworthy, EM Harrison, SJ Wigmore, K Stevenson, D Kingsmore, NS Sheerin, O Bestard, HA Stirnadel-, L Abberley, M Busz, S DeWall, M Birchler, D Krull, KS Thorneloe, A Weber, L Devey
PLoS ONE, 2021-03-08;16(3):e0247972.
Species: Human
Sample Types: Serum
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IL-18BP is a secreted immune checkpoint and barrier to IL-18 immunotherapy
Authors: T Zhou, W Damsky, OE Weizman, MK McGeary, KP Hartmann, CE Rosen, S Fischer, R Jackson, RA Flavell, J Wang, MF Sanmamed, MW Bosenberg, AM Ring
Nature, 2020-06-24;0(0):.
Species: Human
Sample Types: Plasma
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Quartz Dust Exposure Affects NLRP3 Inflammasome Activation and Plasma Levels of IL-18 and IL-1Ra in Iron Foundry Workers
Authors: A Hedbrant, L Andersson, IL Bryngelsso, D Eklund, H Westberg, E Särndahl, A Persson
Mediators Inflamm., 2020-01-07;2020(0):8490908.
Species: Human
Sample Types: Plasma
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Whole blood assay as a model for in vitro evaluation of inflammasome activation and subsequent caspase-mediated interleukin-1 beta release
Authors: TAT Tran, HW Grievink, K Lipinska, C Kluft, J Burggraaf, M Moerland, D Tasev, KE Malone
PLoS ONE, 2019-04-08;14(4):e0214999.
Species: Human
Sample Types: Cell Culture Supernates
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Evaluation of the Effect Derived from Silybin with Vitamin D and Vitamin E Administration on Clinical, Metabolic, Endothelial Dysfunction, Oxidative Stress Parameters, and Serological Worsening Markers in Nonalcoholic Fatty Liver Disease Patients
Authors: A Federico, M Dallio, M Masarone, AG Gravina, R Di Sarno, C Tuccillo, V Cossiga, S Lama, P Stiuso, F Morisco, M Persico, C Loguercio
Oxid Med Cell Longev, 2019;2019(0):8742075.
Species: Human
Sample Types: Serum
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Aberrant monocyte responses predict and characterize dengue virus infection in individuals with severe disease
Authors: YK Yong, HY Tan, SH Jen, EM Shankar, SK Natkunam, J Sathar, R Manikam, SD Sekaran
J Transl Med, 2017-05-31;15(1):121.
Species: Human
Sample Types: Plasma
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Human Mucosa-Associated Invariant T Cells Accumulate in Colon Adenocarcinomas but Produce Reduced Amounts of IFN-gamma.
Authors: Sundstrom P, Ahlmanner F, Akeus P, Sundquist M, Alsen S, Yrlid U, Borjesson L, Sjoling A, Gustavsson B, Wong S, Quiding-Jarbrink M
J Immunol, 2015-08-21;195(7):3472-81.
Species: Human
Sample Types: Cell Culture Supernates
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Reviews for Human IL-18 BPa Quantikine ELISA Kit
Average Rating: 5 (Based on 1 Review)
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Human IL-18 BPa Quantikine ELISA Kit
By Milos Lazic
on 05/08/2018
Sample Tested: Kupffer cells
Very good ELISA. Have been using it only in cell supernatants. Linear standard curve, easy to calculate IL-18 concentrations. Appropriate for concentration response curves. Used it instead of HTRF, due to higher sensitivity!
