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Human Growth Hormone ELISA Kit - Quantikine

Catalog #: DGH00
12 Citations 3 Reviews Datasheet / COA / SDS
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QC99: Quantikine Immunoassay Control Set 1076 Human Growth Hormone
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Human Growth Hormone ELISA Calibrator Diluent RD5K Standard Curve
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Citations (12)
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Reviews (3)
Summary Sample Values Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Growth Hormone Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
7.18 pg/mL
Assay Range
25.0 - 1,600 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Growth Hormone
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Sample Values

Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence of human Growth Hormone in this assay. No medical histories were available for the donors used in this study.

FemalesMean of Detectable (pg/mL)% DetectableRange (pg/mL)
Serum (n=18)55010029.3-4360
EDTA plasma (n=18)64810030.2-5936
Heparin plasma (n=18)64110038.1-5392

MalesMean of Detectable (pg/mL)% DetectableRange (pg/mL)
Serum (n=17)62.083ND-120.5
EDTA plasma (n=17)56.486ND-99.2
Heparin plasma (n=17)58.186ND-109.6
ND=Non-detectable

Cell culture Supernates - - Human peripheral blood cells (1 x 106 cells/mL) were cultured in DMEM supplemented with 5% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates were removed and assayed for levels of human Growth Hormone. No detectable levels were observed.




Product Summary

The Quantikine Human Growth Hormone Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Growth Hormone in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Growth Hormone and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Growth Hormone showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Growth Hormone.

Recovery

The recovery of Growth Hormone spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 97-105
EDTA Plasma (n=4) 108 101-117
Heparin Plasma (n=4) 107 99-117
Serum (n=4) 105 98-111

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Growth Hormone were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Growth Hormone ELISA Linearity

Scientific Data

N/A Human Growth Hormone ELISA Calibrator Diluent RD5K Standard Curve View Larger

Human Growth Hormone ELISA Calibrator Diluent RD5K Standard Curve

N/A Human Growth Hormone ELISA Calibrator Diluent RD6-15 Standard Curve View Larger

Human Growth Hormone ELISA Calibrator Diluent RD6-15 Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Growth Hormone

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretory granules.

Entrez Gene IDs:
2688 (Human)
Alternate Names:
GH1; GHB5; GHIGHD1B; GHN; GH-N; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; Growth Hormone; hGH-N; IGHD1A; IGHD2; Somatotropin
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Growth Hormone Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Magnetically actuating implantable pump for the on-demand and needle-free administration of human growth hormone
    Authors: SH Lee, CR Kim, YC Cho, SN Kim, BH Kim, C Lee, HB Ji, JH Han, CG Park, H Hong, YB Choy
    International journal of pharmaceutics, 2022-03-12;618(0):121664.
    Species: Rat
    Sample Types: Plasma
  2. Local non-pituitary growth hormone is induced with aging and facilitates epithelial damage
    Authors: V Chesnokova, S Zonis, A Apostolou, HQ Estrada, S Knott, K Wawrowsky, K Michelsen, A Ben-Shlomo, R Barrett, V Gorbunova, K Karalis, S Melmed
    Cell Reports, 2021-12-14;37(11):110068.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. High-throughput mediation analysis of human proteome and metabolome identifies mediators of post-bariatric surgical diabetes control
    Authors: JM Dreyfuss, Y Yuchi, X Dong, V Efthymiou, H Pan, DC Simonson, A Vernon, F Halperin, P Aryal, A Konkar, Y Sebastian, BW Higgs, J Grimsby, CM Rondinone, S Kasif, BB Kahn, K Foster, R Seeley, A Goldfine, V Djordjilov, ME Patti
    Nature Communications, 2021-11-29;12(1):6951.
    Species: Human
    Sample Types: Plasma
  4. Structural and functional characterization of recombinant human growth hormone isolated from transgenic pig milk
    Authors: SY Lee, JH Han, EK Lee, YK Kim, SA Hwang, SH Lee, M Kim, GY Cho, JH Hwang, SJ Kim, JG Yoo, SK Cho, KJ Lee, WK Cho
    PLoS ONE, 2020-07-31;15(7):e0236788.
    Species: Porcine
    Sample Types: Cell Culture Supernates, Milk
  5. Investigating Stress Response during Vaginal Delivery and Elective Cesarean Section through Assessment of Levels of Cortisol, Interleukin 6 (IL-6), Growth Hormone (GH) and Insulin-Like Growth Factor 1 (IGF-1)
    Authors: N Kiriakopou, S Grigoriadi, E Maziotis, A Philippou, A Rapani, P Giannelou, P Tsioulou, K Sfakianoud, A Kontogeorg, P Bakas, G Mastorakos, M Koutsilier, M Simopoulou
    J Clin Med, 2019-07-27;8(8):.
    Species: Human
    Sample Types: Serum
  6. Metabolomics in plasma of Malawian children 7?years after surviving severe acute malnutrition: "ChroSAM" a cohort study
    Authors: C Bourdon, N Lelijveld, D Thompson, PS Dalvi, GB Gonzales, D Wang, M Alipour, E Wine, E Chimwezi, JC Wells, M Kerac, R Bandsma, MJ Nyirenda
    EBioMedicine, 2019-06-27;0(0):.
    Species: Human
    Sample Types: Plasma
  7. Implantable multireservoir device with stimulus-responsive membrane for on-demand and pulsatile delivery of growth hormone
    Authors: SH Lee, H Piao, YC Cho, SN Kim, G Choi, CR Kim, HB Ji, CG Park, C Lee, CI Shin, WG Koh, YB Choy, JH Choy
    Proc. Natl. Acad. Sci. U.S.A., 2019-05-23;0(0):.
    Species: Rat
    Sample Types: Plasma
  8. Relative Bioavailability of a Single 4-mg Dose of Somatropin Administered by Subcutaneous Injection or by Needle-free Device and Coadministered With the Growth Hormone Inhibitor Octreotide Acetate in Healthy Adult Subjects
    Authors: DB Brimhall, N Petri, P D'Angelo
    Clin Ther, 2018-04-24;0(0):.
    Species: Human
    Sample Types: Serum
  9. Elevations in growth hormone and glucagon-like peptide-2 levels on admission are associated with increased mortality in trauma patients.
    Authors: Matthew P Rowan, Darrick J Beckman, Julie A Rizzo, Claire L Isbell, Christopher E White, Stephen M Cohn, Kevin K Chung
    Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine, 2016-10-04;0(0):1757-7241.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  10. Endocrine responses during overnight recovery from exercise: impact of nutrition and relationships with muscle protein synthesis.
    Authors: Betts JA, Beelen M, Stokes KA, Saris W HM, van Loon L JC
    Int J Sport Nutr Exerc Metab, 2011-08-10;21(5):398-409.
    Species: Human
    Sample Types: Plasma
  11. Skeletal muscle myofibrillar and sarcoplasmic protein synthesis rates are affected differently by altitude-induced hypoxia in native lowlanders.
    Authors: Holm L, Haslund ML, Robach P, van Hall G, Calbet JA, Saltin B, Lundby C
    PLoS ONE, 2010-12-20;5(12):e15606.
    Species: Human
    Sample Types: Plasma
  12. CYP2C19 pharmacogenetics in advanced cancer: compromised function independent of genotype.
    Authors: Helsby NA, Lo WY, Sharples K, Riley G, Murray M, Spells K, Dzhelai M, Simpson A, Findlay M
    Br. J. Cancer, 2008-10-21;99(8):1251-5.
    Species: Human
    Sample Types: Serum

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