Human Granzyme B Quantikine ELISA Kit Summary
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL)
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates)
Specificity
natural and recombinant human Granzyme B.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® Human Granzyme B Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human Granzyme B in culture supernates. It contains NS0-expressed recombinant human Granzyme B and antibodies raised against the recombinant protein. Results obtained using natural human Granzyme B showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Granzyme B
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians
Cell Culture Supernates
|
Intra-Assay Precision |
Inter-Assay Precision |
| Sample |
1 |
2 |
3 |
1 |
2 |
3 |
| n |
20 |
20 |
20 |
20 |
20 |
20 |
| Mean (pg/mL) |
312 |
630 |
1298 |
311 |
615 |
1208 |
| Standard Deviation |
8.04 |
11 |
28.7 |
27.5 |
56.1 |
77.2 |
| CV% |
2.6 |
1.7 |
2.2 |
8.8 |
9.1 |
6.4 |
Recovery
The recovery of human Granzyme B spiked to levels throughout the range of the assay was evaluated.
| Sample Type |
Average % Recovery |
Range % |
| Cell Culture Media (n=4) |
103 |
93-114 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Granzyme B were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Human Granzyme B Quantikine ELISA Kit Linearity Competitor Comparison
Human Granzyme B Standard Curve
Product Datasheets
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Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Granzyme B
Granzyme B is a member of the granzyme family of serine proteases found specifically in
granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays
an essential role in granule-mediated apoptosis utilizing the substrates in this pathway, such as
Caspase 3, Caspase 8 and Bid (3, 4). Recent research indicates expanded Granzyme B
functionality to include extracellular roles along with its classical pro-apoptotic function. It has
been found that Granzyme B is an important mediator of skin injury, repair and inflammation
(4) through extracellular substrates including Laminin, VE-Cadherin, Fibronectin and the
proteoglycans Aggrecan (3) and Decorin (4).
As one of the five Granzymes (A, B, H, K and M) identified in the human genome, Granzyme B
(32kDa) (5) is the most widely researched in terms of its biological function and its utility in
health and disease (4). It is synthesized as a precursor (247 residues) with a signal peptide
(residues 1-18), a pro-peptide (residues 19-20), and a mature chain (residues 21-247) (6-8).
Once inside granules, Granzyme B is fully processed into the mature chain and becomes an
active protease when the pro-peptide, Gly-Glu is removed from the N-terminus by cleavage
with Cathepsin C (9). The protease activity of Granzyme B is tightly controlled by Serpin B9/
Protease Inhibitor 9 (9). The amino acid sequence of human Granzyme B is 71%, 69%, and 68%
identical to its canine, rat, and mouse counterparts, respectively.
Granzymes have been shown to modulate inflammation, and Granzyme B plasma levels have
been found higher with atopic dermatitis and psoriasis when compared to healthy controls.
This is in contrast to Granzyme A plasma levels which remain unchanged (10). Serum from
patients with Crohn's disease have significantly higher Granzyme B levels than controls (11).
Entrez Gene IDs:
3002 (Human); 14939 (Mouse)
Alternate Names:
C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; Fragmentin-2; Granzyme B; Granzyme-2; GranzymeB; GRB; GrzB; GZMB; HLP; SECT
Citation for Human Granzyme B Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
1
Citation: Showing 1 - 1
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Chemotherapy-induced high expression of IL23A enhances efficacy of anti-PD-1 therapy in TNBC by co-activating the PI3K-AKT signaling pathway of CTLs
Authors: Pan, F;Liu, J;Chen, Y;Zhu, B;Chen, W;Yang, Y;Zhu, C;Zhao, H;Liu, X;Xu, Y;Xu, X;Huo, L;Xie, L;Wang, R;Gu, J;Huang, G;
Scientific reports
Species: Human
Sample Types: Cell Culture Supernates
