Human HB-EGF Quantikine ELISA Kit Summary
Product Summary
human HB-EGF.
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 82 | 186 | 328 | 76 | 171 | 313 |
| Standard Deviation | 2.82 | 2.3 | 2.5 | 7.31 | 10.1 | 22.2 |
| CV% | 3.4 | 1.2 | 0.8 | 9.6 | 5.9 | 7.1 |
Recovery
The recovery of human HB-EGF spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 120 | 113-125 |
| EDTA Plasma (n=4) | 99 | 83-114 |
| Heparin Plasma (n=4) | 84 | 75-96 |
| Platelet-poor EDTA Plasma (n=4) | 101 | 84-119 |
| Platelet-poor Heparin Plasma (n=4) | 98 | 75-111 |
| Serum (n=4) | 95 | 79-107 |
| Urine (n=4) | 98 | 81-121 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: HB-EGF
HB-EGF (Heparin-Binding EGF-like growth factor), also known as DTR, is produced by bronchial epithelium, visceral and vascular smooth muscle, CD4+ T cells, cardiac muscle, glomerular podocytes, keratinocytes, and IL-10-secreting regulatory macrophages. It activates both the EGF R/ErbB1 and ErbB4 receptors and has been linked to many cellular processes including proliferation, apoptosis, cell migration/invasion, differentiation, morphogenesis, and development. HB-EGF is also involved in several aspects of cancer development and progression.
Citations for Human HB-EGF Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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ERBB4-mediated signaling is a mediator of resistance to BTK and PI3K inhibitors in B cell lymphoid neoplasms
Authors: AJ Arribas, S Napoli, L Cascione, L Barnabei, G Sartori, E Cannas, E Gaudio, C Tarantelli, AA Mensah, F Spriano, A Zucchetto, FM Rossi, A Rinaldi, MC de Moura, S Jovic, RB Pittau, A Stathis, G Stussi, V Gattei, JR Brown, M Esteller, E Zucca, D Rossi, F Bertoni
bioRxiv : the preprint server for biology, 2023-01-02;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Serum Concentration of Selected Angiogenesis-Related Molecules Differs among Molecular Subtypes, Body Mass Index and Menopausal Status in Breast Cancer Patients
Authors: D Balalis, D Tsakogiann, E Kalogera, S Kokkali, E Tripodaki, A Ardavanis, D Manatakis, D Dimas, N Koufopoulo, F Fostira, D Korkolis, I Misitzis, N Vassos, C Spiliopoul, D Vlachodimi, G Bletsa, N Arkadopoul
Oncogene, 2022-07-14;11(14):.
Species: Human
Sample Types: Serum
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Forkhead Box q1 promotes invasion and metastasis in colorectal cancer by activating the epidermal growth factor receptor pathway
Authors: JJ Zhang, CX Cao, LL Wan, W Zhang, ZJ Liu, JL Wang, Q Guo, H Tang
World Journal of Gastroenterology, 2022-05-07;28(17):1781-1797.
Species: Human
Sample Types: Cell Culture Supernates
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Serum Amphiregulin and Heparin-Binding Epidermal Growth Factor as Biomarkers in Patients with Idiopathic Inflammatory Myopathy
Authors: N Hanata, Y Nagafuchi, Y Sugimori, S Kobayashi, Y Tsuchida, Y Iwasaki, H Shoda, K Fujio
Journal of Clinical Medicine, 2021-08-22;10(16):.
Species: Human
Sample Types: Serum
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