Home / IL-17F / Human IL-17F DuoSet ELISA

Human IL-17F DuoSet ELISA

Catalog #: DY1335B
13 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY1335B-05
DY1335B
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human IL-17F ELISA Standard Curve
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Product Details
Procedure
Citations (13)
FAQs
Supplemental Products
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human IL-17F DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Range
12.5 - 800 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Interleukin 17F (IL-17F). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, and plasma samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

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Human IL-17F ELISA Standard Curve

N/A Human IL-17F ELISA Standard Curve View Larger

Human IL-17F ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17F

The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.

Long Name:
Interleukin 17F
Entrez Gene IDs:
112744 (Human); 257630 (Mouse); 301291 (Rat); 708220 (Rhesus Macaque)
Alternate Names:
Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; interleukin 17F

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human IL-17F DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Differential impacts of TNFalpha inhibitors on the transcriptome of Th cells
    Authors: CH Ho, AA Silva, B Tomita, HY Weng, IC Ho
    Arthritis Research & Therapy, 2021-07-23;23(1):199.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. The DHODH Inhibitor PTC299 Arrests SARS-CoV-2 Replication and Suppresses Induction of Inflammatory Cytokines
    Authors: J Luban, R Sattler, E Mühlberger, JD Graci, L Cao, M Weetall, C Trotta, JM Colacino, S Bavari, C Strambio-D, EL Suder, Y Wang, V Soloveva, K Cintron-Lu, NA Naryshkin, M Pykett, EM Welch, K O'Keefe, R Kong, E Goodwin, A Jacobson, S Paessler, S Peltz
    Virus Res, 2020-11-26;0(0):198246.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Impact of malnutrition on systemic immune and metabolic profiles in type 2 diabetes
    Authors: A Rajamanick, S Munisankar, CK Dolla, K Thiruvenga, S Babu
    BMC Endocr Disord, 2020-11-12;20(1):168.
    Species: Human
    Sample Types: Plasma
  4. Ex vivo culture of lesional psoriasis skin for pharmacological testing
    Authors: ML Tiirikaine, A Woetmann, H Norsgaard, LF Santamaria, P Lovato
    J. Dermatol. Sci., 2019-12-27;0(0):.
    Species: Human
    Sample Types: Tissue Lysates
  5. Interleukin-17F expression is elevated in hepatitis C patients with fibrosis and hepatocellular carcinoma
    Authors: MS Wu, CH Wang, FC Tseng, HJ Yang, YC Lo, YP Kuo, DJ Tsai, WT Tsai, GY Yu
    Infect. Agents Cancer, 2017-07-26;12(0):42.
    Species: Human
    Sample Types: Serum
  6. Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency.
    Authors: Mazerolles F, Picard C, Kracker S, Fischer A, Durandy A
    J Allergy Clin Immunol, 2013-02-10;131(4):1146-56, 1156.
    Species: Human
    Sample Types: Whole Cells
  7. A naturally occurring, soluble antagonist of human IL-23 inhibits the development and in vitro function of human Th17 cells.
    Authors: Yu RY, Gallagher G
    J. Immunol., 2010-11-12;185(12):7302-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Chronic mucocutaneous candidiasis in APECED or thymoma patients correlates with autoimmunity to Th17-associated cytokines.
    Authors: Kisand K, Boe Wolff AS, Podkrajsek KT, Tserel L, Link M, Kisand KV, Ersvaer E, Perheentupa J, Erichsen MM, Bratanic N, Meloni A, Cetani F, Perniola R, Ergun-Longmire B, Maclaren N, Krohn KJ, Pura M, Schalke B, Strobel P, Leite MI, Battelino T, Husebye ES, Peterson P, Willcox N, Meager A
    J. Exp. Med., 2010-02-01;207(2):299-308.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Opposing roles of IL-17A and IL-25 in the regulation of TSLP production in human nasal epithelial cells.
    Authors: Xu G, Zhang L, Wang DY, Xu R, Liu Z, Han DM, Wang XD, Zuo KJ, Li HB
    Allergy, 2009-11-25;65(5):581-9.
    Species: Human
    Sample Types: Nasal Lavage Fluid
  10. Characterization of the interleukin-17 isoforms and receptors in lesional psoriatic skin.
    Authors: Johansen C, Usher PA, Kjellerup RB
    Br. J. Dermatol., 2008-10-21;160(2):319-24.
    Species: Human
    Sample Types: Tissue Homogenates
  11. Functional characterization of IL-17F as a selective neutrophil attractant in psoriasis.
    Authors: Watanabe H, Kawaguchi M, Fujishima S, Ogura M, Matsukura S, Takeuchi H, Ohba M, Sueki H, Kokubu F, Hizawa N, Adachi M, Huang SK, Iijima M
    J. Invest. Dermatol., 2008-10-02;129(3):650-6.
    Species: Human
    Sample Types: Tissue Homogenates
  12. IL-22 mediates mucosal host defense against Gram-negative bacterial pneumonia.
    Authors: Aujla SJ, Chan YR, Zheng M, Fei M, Askew DJ, Pociask DA, Reinhart TA, McAllister F, Edeal J, Gaus K, Husain S, Kreindler JL, Dubin PJ, Pilewski JM, Myerburg MM, Mason CA, Iwakura Y, Kolls JK
    Nat. Med., 2008-02-10;14(3):275-81.
    Species: Human
    Sample Types: BALF
  13. Linking genetic susceptibility to Crohn's disease with Th17 cell function: IL-22 serum levels are increased in Crohn's disease and correlate with disease activity and IL23R genotype status.
    Authors: Schmechel S, Konrad A, Diegelmann J, Glas J, Wetzke M, Paschos E, Lohse P, Goke B, Brand S
    Inflamm. Bowel Dis., 2008-02-01;14(2):204-12.
    Species: Human
    Sample Types: Serum

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