Home / APRIL/TNFSF13 / Human APRIL/TNFSF13 DuoSet ELISA

Human APRIL/TNFSF13 DuoSet ELISA

Catalog #: DY884B
17 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY884B
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human APRIL/TNFSF13 ELISA Standard Curve
2 Images
Product Details
Citations (17)
FAQs
Supplemental Products
Reviews (1)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human APRIL/TNFSF13 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human APRIL / TNFSF13. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A Human APRIL / TNFSF13 ELISA Standard Curve View Larger

Human APRIL/TNFSF13 ELISA Standard Curve

N/A View Larger

Human APRIL/TNFSF13 ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: APRIL/TNFSF13

A proliferation-inducing ligand (APRIL), also known as TALL-2 and TRDL-1, is a type II transmembrane protein that is a member of the TNF ligand superfamily (TNFSF13). APRIL expression is low in normal tissues, but is elevated in several types of tumors and transformed cell lines. APRIL stimulates proliferation of tumor cell lines and increases tumorigenicity in nude mice.

Long Name:
A Proliferation-inducing Ligand
Entrez Gene IDs:
8741 (Human); 69583 (Mouse); 287437 (Rat)
Alternate Names:
A proliferation-inducing ligand; APRIL; APRILFLJ57090; CD256 antigen; CD256; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; TNFSF13; TRDL1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; ZTNF2

Citations for Human APRIL/TNFSF13 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Association between the expression of Toll-like receptors, cytokines, and homeostatic chemokines in SARS-CoV-2 infection and COVID-19 severity
    Authors: W Alturaiki, H Alkadi, S Alamri, ME Awadalla, A Alfaez, A Mubarak, MA Alanazi, FQ Alenzi, BF Flanagan, B Alosaimi
    Heliyon, 2022-12-24;0(0):e12653.
    Species: Human
    Sample Types: Serum
  2. A Proliferation-Inducing Ligand and B-Cell Activating Factor Are Upregulated in Patients with Essential Thrombocythemia
    Authors: L Bolkun, M Tynecka, T Wasiluk, J Piszcz, A Starosz, K Grubczak, M Moniuszko, A Eljaszewic
    Journal of Clinical Medicine, 2022-08-09;11(16):.
    Species: Human
    Sample Types: Serum
  3. Antibody microarray analysis of amniotic fluid proteomes in women with cervical insufficiency and short cervix, and their association with pregnancy latency length
    Authors: S Hong, KH Park, YE Lee, JE Lee, YM Kim, E Joo, I Cho
    PLoS ONE, 2022-02-07;17(2):e0263586.
    Species: Human
    Sample Types: Amniotic Fluid
  4. Plasma levels of BAFF and APRIL are elevated in patients with asthma in Saudi Arabia
    Authors: W Alturaiki, A Mubarak, SA Mir, A Afridi, M Premanatha, S Mickymaray, R Vijayakuma, SA Alsagaby, SG Almalki, F Alghofaili, AK Alnemare, BF Flanagan
    Saudi journal of biological sciences, 2021-08-23;28(12):7455-7459.
    Species: Human
    Sample Types: Plasma
  5. Characterization of B- and T-Cell Compartment and B-Cell Related Factors Belonging to the TNF/TNFR Superfamily in Patients With Clinically Active Systemic Lupus Erythematosus: Baseline BAFF Serum Levels Are the Strongest Predictor of Response to Belimumab after Twelve Months of Therapy
    Authors: S Piantoni, F Regola, S Masneri, M Merletti, T Lowin, P Airò, A Tincani, F Franceschi, L Andreoli, G Pongratz
    Frontiers in Pharmacology, 2021-05-21;12(0):666971.
    Species: Human
    Sample Types: Serum
  6. Immune and Inflammatory Proteins in Cord Blood as Predictive Biomarkers of Retinopathy of Prematurity in Preterm Infants
    Authors: YJ Park, SJ Woo, YM Kim, S Hong, YE Lee, KH Park
    Invest. Ophthalmol. Vis. Sci., 2019-09-03;60(12):3813-3820.
    Species: Human
    Sample Types: Plasma
  7. B cell maturation antigen-specific CAR T cells are clinically active in multiple myeloma.
    Authors: Cohen A, Garfall A, Stadtmauer E, Melenhorst J, Lacey S, Lancaster E, Vogl D, Weiss B, Dengel K, Nelson A, Plesa G, Chen F, Davis M, Hwang W, Young R, Brogdon J, Isaacs R, Pruteanu-Malinici I, Siegel D, Levine B, June C, Milone M
    J Clin Invest, 2019-03-21;130(0):.
    Species: Human
    Sample Types: Serum (confirm CAR-T)
  8. BAFF-R and TACI expression on CD3+ T cells: Interplay among BAFF, APRIL and T helper cytokines profile in systemic lupus erythematosus
    Authors: DC Salazar-Ca, P Ortíz-Laza, M Marín-Rosa, A Cruz, F Muñoz-Vall, R Tapia-Llan, G Orozco-Bar, R Machado-Co, CA Palafox-Sá
    Cytokine, 2018-11-19;0(0):.
    Species: Human
    Sample Types: Serum
  9. Altered circulating levels of B cell growth factors and their modulation upon anti-tuberculosis treatment in pulmonary tuberculosis and tuberculous lymphadenitis
    Authors: GR Kathamuthu, K Moideen, VV Banurekha, D Nair, R Sridhar, D Baskaran, S Babu
    PLoS ONE, 2018-11-14;13(11):e0207404.
    Species: Human
    Sample Types: Plasma
  10. Serum BAFF in Indian patients with IIM: a retrospective study reveals novel clinico-phenotypic associations in children and adults
    Authors: L Gupta, S Chaurasia, P Srivastava, S Dwivedi, A Lawrence, R Misra
    Clin. Rheumatol., 2018-03-07;0(0):.
    Species: Human
    Sample Types: Serum
  11. Abnormal differentiation of B cells and megakaryocytes in Roifman syndrome
    Authors: J Heremans, JE Garcia-Per, E Turro, SM Schlenner, I Casteels, R Collin, F de Zegher, D Greene, S Humblet-Ba, S Lesage, P Matthys, CJ Penkett, K Put, K Stirrups, C Thys, C Van Geet, E Van Nieuwe, C Wouters, I Meyts, K Freson, A Liston
    J. Allergy Clin. Immunol., 2018-01-31;0(0):.
    Species: Human
    Sample Types: Serum
  12. Modulation of Mycobacterium tuberculosis-specific humoral immune responses is associated with Strongyloides stercoralis co-infection
    Authors: R Anuradha, S Munisankar, Y Bhootra, C Dolla, P Kumaran, TB Nutman, S Babu
    PLoS Negl Trop Dis, 2017-05-01;11(5):e0005569.
    Species: Human
    Sample Types: Plasma
  13. Inflamm-aging influences immune cell survival factors in human bone marrow
    Eur. J. Immunol, 2017-01-11;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Urinary B cell Activating Factor (BAFF) and A Proliferation Inducing Ligand (APRIL): Potential biomarkers of Active Lupus Nephritis
    Authors: Ramnath Misra
    Clin. Exp. Immunol., 2016-12-05;0(0):.
    Species: Human
    Sample Types: Serum
  15. Utility of CSF Cytokine/Chemokines as Markers of Active Intrathecal Inflammation: Comparison of Demyelinating, Anti-NMDAR and Enteroviral Encephalitis
    PLoS ONE, 2016-08-30;11(8):e0161656.
    Species: Human
    Sample Types: CSF
  16. Selective activation of human dendritic cells by OM-85 through a NF-kB and MAPK dependent pathway.
    Authors: Parola C, Salogni L, Vaira X, Scutera S, Somma P, Salvi V, Musso T, Tabbia G, Bardessono M, Pasquali C, Mantovani A, Sozzani S, Bosisio D
    PLoS ONE, 2013-12-30;8(12):e82867.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Study of the quantitative, functional, cytogenetic, and immunoregulatory properties of bone marrow mesenchymal stem cells in patients with B-cell chronic lymphocytic leukemia.
    Authors: Pontikoglou C, Kastrinaki M, Klaus M, Kalpadakis C, Katonis P, Alpantaki K, Pangalis G, Papadaki H
    Stem Cells Dev, 2013-02-01;22(9):1329-41.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human APRIL/TNFSF13 DuoSet ELISA
By Marry Tahani on 01/09/2019
Sample Tested: Serum
Human APRIL/TNFSF13 DuoSet ELISA DY884B