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Sialyltransferase Activity Kit

Catalog #: EA002
6 Citations Datasheet / COA / SDS
Measurement of sialyltransferase activity
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EA002
R&D Systems Glycosyltransferase Activity Assays
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Citations (6)
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Sialyltransferase Activity Kit Summary

View Full Assay Principle
The Sialyltransferase Activity Kit (Catalog # EA002) provides a simple, non-radioactive high-throughput compatible format for assaying the enzyme activity of all sialyltransferases regardless of different acceptor substrates. This kit utilizes the 5’-nucleotidase CD73 as a coupling phosphatase to remove inorganic phosphate quantitatively from the leaving nucleotide cytidine 5’-monophosphate (CMP) that is generated during sialyltransferase reactions.

 

Features

  • Applicable for all sialyltransferases
  • Non-radioactive
  • No time-consuming separation steps (e.g. column chromatography)
  • Provides accurate kinetic analysis of sialyltransferase reactions
  • Amenable to high-throughput analysis

Kit Contents

  • CHO cell-expressed Recombinant Human CD73
  • CMP
  • Phosphatase Buffer 2
  • 100 mM MnCl2
  • Phosphate Standard
  • Malachite Green Reagents A & B

Data Example

Activity of ST6GALNAC2

Activity of ST6GALNAC2View Larger Image

The ability of Recombinant Mouse ST6 Sialyltransferase 2/ST6GALNAC2 (Catalog # 6468-GT) to transfer Neu5Ac (sialic acid) from CMP-Neu5Ac to bovine asialofetuin was measured using the Sialyltransferase Activity Kit (Catalog # EA002). The activity was plotted against enzyme input, and the specific activity of ST6GALNAC2 was determined to be 774 pmol/min/μg.

Specifications

Source
N/A
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-Species

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Product Datasheet
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Citations for Sialyltransferase Activity Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans
    Authors: T Jaroentome, YH Kwon, Y Liu, O Young, R Bhawal, JD Wilson, M Li, DG Chapla, KW Moremen, MC Jewett, D Mizrachi, MP DeLisa
    Nature Communications, 2022-10-24;13(1):6325.  2022-10-24
  2. Transfer of Functional Cargo in Exomeres
    Authors: Q Zhang, JN Higginboth, DK Jeppesen, YP Yang, W Li, ET McKinley, R Graves-Dea, J Ping, CM Britain, KA Dorsett, CL Hartman, DA Ford, RM Allen, KC Vickers, Q Liu, JL Franklin, SL Bellis, RJ Coffey
    Cell Rep, 2019-04-04;0(0):.  2019-04-04
  3. Engineering of CHO cells for the production of vertebrate recombinant sialyltransferases
    Authors: B Houeix, MT Cairns
    PeerJ, 2019-02-11;7(0):e5788.  2019-02-11
  4. B-cell-independent sialylation of IgG
    Authors: Mark B Jones
    Proc Natl Acad Sci USA, 2016-06-14;113(26):7207-12.  2016-06-14
  5. Mutation in ST6GALNAC5 identified in family with coronary artery disease.
    Authors: InanlooRahatloo, Kolsoum, Parsa, Amir Far, Huse, Klaus, Rasooli, Paniz, Davaran, Saeid, Platzer, Matthias, Kramer, Marcel, Fan, Jian-Bin, Turk, Casey, Amini, Sasan, Steemers, Frank, Gunderson, Kevin, Ronaghi, Mostafa, Elahi, Elahe
    Sci Rep, 2014-01-08;4(0):3595.  2014-01-08
  6. Selective exo-enzymatic labeling of N-glycans on the surface of living cells by recombinant ST6Gal I.
    Authors: Mbua N, Li X, Flanagan-Steet H, Meng L, Aoki K, Moremen K, Wolfert M, Steet R, Boons G
    Angew Chem Int Ed Engl, 2013-10-15;52(49):13012-5.  2013-10-15

FAQs

  1. Does the Malachite Green Phosphate Detection Kit (DY996) detect both pyrophosphate (diphosphate) and free phosphate (monophosphate)?

    • The Malachite Green Phosphate Detection Kit measures free inorganic phosphate in aqueous solutions. The assay principle is based on complex formation between malachite green molybdate and free phosphate under acidic conditions. Lipid bound, protein-bound, pyrophashate and other condensed phosphates must be hydrolyzed prior to measurement in the malachite green assay.

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