Rabbit IgG APC-conjugated Antibody

Catalog # Availability Size / Price Qty
F0111
Detection of Rabbit IgG Primary Antibody by Flow Cytometry
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Product Details
Citations (12)
FAQs
Supplemental Products
Reviews (3)

Rabbit IgG APC-conjugated Antibody Summary

Species Reactivity
Rabbit
Specificity
Rabbit IgG specific IgG was purified by rabbit IgG affinity chromatography.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Rabbit IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of rabbit origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Rabbit IgG Primary Antibody by Flow Cytometry View Larger

Detection of Rabbit IgG Primary Antibody by Flow Cytometry HEK293 human embryonic kidney cell line transfected with V5-tagged proteins was stained with Rabbit Anti-V5 Epitope Tag Monoclonal Antibody (Catalog # MAB8926, filled histogram) or isotype control antibody (Catalog # AB-105-C), open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111) at 10 µL/106cells.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Rabbit IgG APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Polymer stealthing and mucin-1 retargeting for enhanced pharmacokinetics of an oncolytic vaccinia virus
    Authors: Claudia Hill, Megan Grundy, Luca Bau, Sheena Wallington, Joel Balkaran, Victor Ramos et al.
    Molecular Therapy - Oncolytics
  2. Effects of statins on the inducible degrader of low-density lipoprotein receptor in familial hypercholesterolemia
    Authors: Melody Lok-Yi Chan, Sammy Wing-Ming Shiu, Ching-Lung Cheung, Anskar Yu-Hung Leung, Kathryn Choon-Beng Tan
    Endocrine Connections
  3. FOXD1 promotes chemotherapy resistance by enhancing cell stemness in colorectal cancer through beta ‑catenin nuclear localization
    Authors: Wen-Qing Feng, Yu-Chen Zhang, Han Gao, Wen-Chang Li, Yi-Ming Miao, Zi-Feng Xu et al.
    Oncology Reports
  4. Targeting Tumor Vascular CD99 Inhibits Tumor Growth
    Authors: Elisabeth J. M. Huijbers, Inge M. van der Werf, Lisette D. Faber, Lena D. Sialino, Pia van der Laan, Hanna A. Holland et al.
    Frontiers in Immunology
  5. Autotaxin in encephalitogenic CD4 T cells as a therapeutic target for multiple sclerosis
    Authors: Petersen-Cherubini, CL;Murphy, SP;Xin, M;Liu, Y;Deffenbaugh, JL;Jahan, I;Rau, CN;Yang, Y;Lovett-Racke, AE;
    European journal of immunology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Prosaposin maintains lipid homeostasis in dopamine neurons and counteracts experimental parkinsonism in rodents
    Authors: He, Y;Kaya, I;Shariatgorji, R;Lundkvist, J;Wahlberg, LU;Nilsson, A;Mamula, D;Kehr, J;Zareba-Paslawska, J;Biverstål, H;Chergui, K;Zhang, X;Andren, PE;Svenningsson, P;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Optic nerve regeneration screen identifies multiple genes restricting adult neural repair
    Authors: JA Lindborg, NM Tran, DM Chenette, K DeLuca, Y Foli, R Kannan, Y Sekine, X Wang, M Wollan, IJ Kim, JR Sanes, SM Strittmatt
    Cell Reports, 2021-03-02;34(9):108777.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. CD4+ T Cell-Derived NGAL Modifies the Outcome of Ischemic Acute Kidney Injury
    Authors: SA Lee, S Noel, JT Kurzhagen, M Sadasivam, PM Pierorazio, LJ Arend, AR Hamad, H Rabb
    J. Immunol., 2019-12-30;204(3):586-595.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Heparin Forms Polymers with Cell-free DNA Which Elongate Under Shear in Flowing Blood
    Authors: JC de Vries, AD Barendrech, CC Clark, RT Urbanus, P Boross, S de Maat, C Maas
    Sci Rep, 2019-12-04;9(1):18316.
    Species: Human
    Sample Types: Citrated Blood
    Applications: IHC
  10. Modeling the Pathological Long-Range Regulatory Effects of Human Structural Variation with Patient-Specific hiPSCs
    Authors: M Laugsch, M Bartusel, R Rehimi, H Alirzayeva, A Karaolidou, G Crispatzu, P Zentis, M Nikolic, T Bleckwehl, P Kolovos, WFJ van Ijcken, T Šari?, K Koehler, P Frommolt, K Lachlan, J Baptista, A Rada-Igles
    Cell Stem Cell, 2019-04-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Plasmacytoid dendritic cells control lung inflammation and monocyte recruitment in indirect acute lung injury in mice.
    Authors: Venet F, Huang X, Chung CS, Chen Y, Ayala A
    Am. J. Pathol., 2009-12-30;176(2):764-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. IL-6 Trans-Signaling Drives Murine Crescentic GN.
    Authors: Braun GS, Nagayama Y, Maruta Y et al.
    J. Am. Soc. Nephrol.

FAQs

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Reviews for Rabbit IgG APC-conjugated Antibody

Average Rating: 5 (Based on 3 Reviews)

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Rabbit IgG APC-conjugated Antibody
By Anonymous on 11/20/2020
Application: Flow Sample Tested: HEK293 human embryonic kidney cell line Species: Human

Rabbit IgG APC-conjugated Antibody
By Danlee Enzler on 05/01/2018
Application: ELISA Sample Tested: Serum Species: Rabbit

Rabbit IgG APC-conjugated Antibody
By Anonymous on 12/01/2017
Application: Flow Sample Tested: Dendritic cells Species: Mouse