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Mouse IgG HRP-conjugated Antibody

Catalog #: HAF018
30 Citations 2 Reviews Datasheet / COA / SDS
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HAF018
Detection of Mouse IgG Primary Antibody by Western Blot
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Citations (30)
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Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IgG HRP-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with human IgG, rabbit IgG and chicken IgY is observed.
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse IgG
Formulation
Supplied as a 0.2 μm filtered solution in PBS with stabilizers.
Label
HRP (Horseradish Peroxidase)(Excitation= nm, Emission= nm)

Applications

Recommended Concentration
Sample
Western Blot
Use at a 1:1000 dilution.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IgG Primary Antibody by Western Blot View Larger

Detection of Mouse IgG Primary Antibody by Western Blot Western blot shows detection of Mouse Anti-Human/Mouse/Rat IGSF4C/SynCAM4 Monoclonal Antibody (Catalog # MAB41642) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Mouse IgG HRP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Integrin – Dependent Mechanotransduction in Mechanically Stimulated Human Annulus Fibrosus Cells: Evidence for an Alternative Mechanotransduction Pathway Operating with Degeneration
    Authors: Hamish T. J. Gilbert, Navraj S. Nagra, Anthony J. Freemont, Sarah J. Millward-Sadler, Judith A. Hoyland
    PLoS ONE
  2. Evaluation of the Role of ITGBL1 in Ovarian Cancer
    Authors: Alexander Jorge Cortez, Katarzyna Aleksandra Kujawa, Agata Małgorzata Wilk, Damian Robert Sojka, Joanna Patrycja Syrkis, Magdalena Olbryt et al.
    Cancers (Basel)
  3. Novel Designed Proteolytically Resistant VEGF-B186R127S Promotes Angiogenesis in Mouse Heart by Recruiting Endothelial Progenitor Cells
    Authors: Rahul Mallick, Erika Gurzeler, Pyry I. Toivanen, Tiina Nieminen, Seppo Ylä-Herttuala
    Frontiers in Bioengineering and Biotechnology
  4. Multi-Material Tissue Engineering Scaffold with Hierarchical Pore Architecture
    Authors: Kathy Ye Morgan, Demetra Sklaviadis, Zachary L. Tochka, Kristin M. Fischer, Keith Hearon, Thomas D. Morgan et al.
    Advanced Functional Materials
  5. Monocyte biomarkers define sargramostim treatment outcomes for Parkinson's disease
    Authors: Mai M. Abdelmoaty, Jatin Machhi, Pravin Yeapuri, Farah Shahjin, Vikas Kumar, Katherine E. Olson et al.
    Clinical and Translational Medicine
  6. ADAM9 expression promotes an aggressive lung adenocarcinoma phenotype
    Authors: Céline Mongaret Kossmann, Maxime Annereau, Audrey Thomas-Schoemann, Carole Nicco-Overney, Christiane Chéreau, Frédéric Batteux et al.
    Tumour Biol
  7. TIM-3 increases the abundance of type-2 dendritic cells during Leishmania donovani infection by enhancing IL-10 production via STAT3
    Authors: Manish Mishra, Manisha Yadav, Sandeep Kumar, Raj Kumar, Pradip Sen
    Cell Death & Disease
  8. Retinal Distribution and Extracellular Activity of Granzyme B: A Serine Protease That Degrades Retinal Pigment Epithelial Tight Junctions and Extracellular Matrix Proteins
    Authors: Joanne A. Matsubara, Yuan Tian, Jing Z. Cui, Matthew R. Zeglinski, Sho Hiroyasu, Christopher T. Turner et al.
    Frontiers in Immunology
  9. Combination Therapy of Novel Oncolytic Adenovirus with Anti-PD1 Resulted in Enhanced Anti-Cancer Effect in Syngeneic Immunocompetent Melanoma Mouse Model
    Authors: Garofalo M, Bertinato L, Staniszewska M et al.
    Pharmaceutics
  10. Novel ITGB6 variants cause hypoplastic-hypomineralized amelogenesis imperfecta and taurodontism: characterization of tooth phenotype and review of literature
    Authors: Kanokwan Sriwattanapong, Thanakorn Theerapanon, Lawan Boonprakong, Anucharte Srijunbarl, Thantrira Porntaveetus, Vorasuk Shotelersuk
    BDJ Open
  11. Bafilomycin A1 Inhibits HIV-1 Infection by Disrupting Lysosomal Cholesterol Transport
    Authors: Song, B;Korolkova, O;
    Viruses
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. High Concentrations of Nucleotides Prevent Capillary Regression during Hindlimb Unloading by Inhibiting Oxidative Stress and Enhancing Mitochondrial Metabolism of Soleus Muscles in Rats
    Authors: Nakanishi, R;Hashimoto, N;Takuwa, M;Xing, J;Uemura, M;Un Nisa, B;Tanaka, M;Hirabayashi, T;Tanaka, M;Fujino, H;
    Acta histochemica et cytochemica
  13. Alternating current electromagnetic field exposure lessens intramyocellular lipid accumulation due to high-fat feeding via enhanced lipid metabolism in mice
    Authors: Nakanishi, R;Tanaka, M;Nisa, BU;Shimizu, S;Hirabayashi, T;Tanaka, M;Maeshige, N;Roy, RR;Fujino, H;
    PloS one
    Species: Mouse
    Sample Types: Tissue Homogenates
  14. Leishmania donovani Impedes Antileishmanial Immunity by Suppressing Dendritic Cells via the TIM-3 Receptor
    Authors: MN Akhtar, S Kumar, P Sen
    MBio, 2022-08-04;13(4):e0330921.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Neuregulin 1 Drives Morphological and Phenotypical Changes in C2C12 Myotubes: Towards De Novo Formation of Intrafusal Fibres In Vitro
    Authors: Barrett P, Quick TJ, Mudera V, Player DJ.
    Frontiers in Cell and Developmental Biology
  16. Efficient Expression of Soluble Recombinant Protein Fused with Core-Streptavidin in Bacterial Strain with T7 Expression System
    Authors: A Tarar, EM Alyami, CA Peng
    Methods and protocols, 2020-12-01;3(4):.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Western Blot
  17. 2-Hydroxypropyl-gamma-cyclodextrin overcomes NPC1 deficiency by enhancing lysosome-ER association and autophagy
    Authors: A Singhal, ES Krystofiak, WG Jerome, B Song
    Sci Rep, 2020-05-26;10(1):8663.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. 2-O, 3-O desulfated heparin mitigates murine chemotherapy- and radiation-induced thrombocytopenia
    Authors: E Tkaczynski, A Arulselvan, J Tkaczynski, S Avery, L Xiao, B Torok-Stor, K Abrams, NV Rao, G Johnson, TP Kennedy, M Poncz, MP Lambert
    Blood Adv, 2018-04-10;2(7):754-761.
    Species: Mouse
    Sample Types: Protein
    Applications: ELISA Capture
  19. Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity
    Authors: MF Gutknecht, ME Seaman, B Ning, DA Cornejo, E Mugler, PF Antkowiak, CA Moskaluk, S Hu, FH Epstein, KA Kelly
    Nat Commun, 2017-09-15;8(1):552.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Microenvironment inflammatory infiltrate drives growth speed and outcome of hepatocellular carcinoma: a prospective clinical study
    Authors: R Critelli, F Milosa, F Faillaci, R Condello, E Turola, L Marzi, B Lei, F Dituri, S Andreani, P Sighinolfi, P Manni, A Maiorana, C Caporali, F di Benedet, M Del Buono, N De Maria, F Schepis, ML Martinez-C, G Giannelli, E Villa
    Cell Death Dis, 2017-08-24;8(8):e3017.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  21. mTOR Inhibition Rejuvenates the Aging Gingival Fibroblasts through Alleviating Oxidative Stress
    Authors: Y Xia, M Sun, Y Xie, R Shu
    Oxid Med Cell Longev, 2017-07-19;2017(0):6292630.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Polysialic acid regulates sympathetic outflow by facilitating information transfer within the nucleus of the solitary tract
    Authors: P Bokiniec, S Shahbazian, SJ McDougall, BA Berning, D Cheng, IJ Llewellyn-, PG Burke, S McMullan, M Mühlenhoff, H Hildebrand, F Braet, M Connor, NH Packer, AK Goodchild
    J. Neurosci., 2017-06-02;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Functional Assay
  23. Heterozygous Vangl2(Looptail) mice reveal novel roles for the planar cell polarity pathway in adult lung homeostasis and repair
    Authors: T Poobalasin, LL Yates, SA Walker, M Pereira, NY Gross, A Ali, M Kolatsi-Jo, MR Jarvelin, J Pekkanen, E Papakrivop, DA Long, M Griffiths, D Wagner, M Königshoff, M Hind, C Minelli, CM Lloyd, CH Dean
    Dis Model Mech, 2017-02-24;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Acidic pH promotes intervertebral disc degeneration: Acid-sensing ion channel -3 as a potential therapeutic target
    Sci Rep, 2016-11-17;6(0):37360.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Altered mitochondrial function and energy metabolism is associated with a radioresistant phenotype in oesophageal adenocarcinoma.
    Authors: Lynam-Lennon, Niamh, Maher, Stephen, Maguire, Aoife, Phelan, James, Muldoon, Cian, Reynolds, John V, O'Sullivan, Jacintha
    PLoS ONE, 2014-06-26;9(6):e100738.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Broad regulation of matrix and adhesion molecules in THP-1 human macrophages by nitroglycerin.
    Authors: Krishnatry AS, Brazeau DA, Fung HL
    Nitric Oxide, 2009-10-15;22(1):11-7.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  27. Sleeping beauty transposition from nonintegrating lentivirus.
    Authors: Vink CA, Gaspar HB, Gabriel R, Schmidt M, McIvor RS, Thrasher AJ, Qasim W
    Mol. Ther., 2009-05-05;17(7):1197-204.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Adenoviral VEGF-B186R127S gene transfer induces angiogenesis and improves perfusion in ischemic heart
    Authors: Henna Korpela, Olli-Pekka Hätinen, Tiina Nieminen, Rahul Mallick, Pyry Toivanen, Jonna Airaksinen et al.
    iScience
  29. Defining the Innate Immune Responses for SARS-CoV-2-Human Macrophage Interactions
    Authors: Mai M. Abdelmoaty, Pravin Yeapuri, Jatin Machhi, Katherine E. Olson, Farah Shahjin, Vikas Kumar et al.
    Frontiers in Immunology
  30. Neuregulin 1 Drives Morphological and Phenotypical Changes in C2C12 Myotubes: Towards De Novo Formation of Intrafusal Fibres In Vitro
    Authors: Barrett P, Quick TJ, Mudera V, Player DJ.
    Frontiers in Cell and Developmental Biology

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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Reviews for Mouse IgG HRP-conjugated Antibody

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Mouse IgG HRP-conjugated Antibody
By Anonymous on 11/27/2021
Application: WB Sample Tested: Colon tissue,Tissue Lysates Species: Mouse
Western Blot Mouse IgG HRP-conjugated Antibody HAF018
Mouse IgG HRP-conjugated Antibody
By Anonymous on 08/05/2020
Application: WB Sample Tested: HeLa cells,HUVEC human umbilical vein endothelial cells Species: Human
Western Blot Mouse IgG HRP-conjugated Antibody HAF018