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Mouse IgG2A Alexa Fluor® 488-conjugated Antibody

Catalog #: IC003G
16 Citations 1 Review Datasheet / COA / SDS
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IC003G
Detection of Mouse IgG Isotype Control by Flow Cytometry
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Citations (16)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IgG2A Alexa Fluor® 488-conjugated Antibody Summary

Specificity
Selected for use as mouse IgG2A isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG2A Clone # 20102
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Mouse IgG Isotype Control by Flow Cytometry View Larger

Detection of Mouse IgG Control by Flow Cytometry BG01V human embryonic stem cells were stained with Mouse Anti-Human Podocalyxin Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1658G, filled histogram) or Mouse IgG Alexa Fluor® 488-conjugated Isotype Control Antibody (Catalog # IC003G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A

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Citations for Mouse IgG2A Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Upregulation of Somatostatin Receptor Type 2 Improves 177Lu-DOTATATE Therapy in Receptor-Deficient Pancreatic Neuroendocrine Tumor Model
    Authors: Sharma R, Earla B, Baidoo KE et al.
    Molecular cancer therapeutics
  2. Extracellular vesicle-associated tyrosine kinase-like orphan receptors ROR1 and ROR2 promote breast cancer progression
    Authors: Barnabas Irmer, Janes Efing, Lea Elisabeth Reitnauer, Allegra Angenendt, Saskia Heinrichs, Antonia Schubert et al.
    Cell Communication and Signaling
  3. Senescent mesenchymal stem/stromal cells in pre-metastatic bone marrow of untreated advanced breast cancer patients
    Authors: Francisco Raúl RA贚 BORZONE, María Belén BEL蒒 GIORELLO, Leandro Marcelo MARCELO MARTINEZ, María Cecilia CECILIA SANMARTIN, LEONARDO FELDMAN, FEDERICO DIMASE et al.
    Oncology Research
  4. Survival-based CRISPR genetic screens across a panel of permissive cell lines identify common and cell-specific SARS-CoV-2 host factors
    Authors: K Chan, AG Farias, H Lee, F Guvenc, P Mero, KR Brown, H Ward, M Billmann, K Aulakh, A Astori, S Haider, E Marcon, U Braunschwe, S Pu, A Habsid, AH Yan Tong, N Christie-H, P Budylowski, A Ghalami, S Mubareka, F Maguire, A Banerjee, KL Mossman, J Greenblatt, SD Gray-Owen, B Raught, BJ Blencowe, M Taipale, C Myers, J Moffat
    Heliyon, 2022-12-30;0(0):e12744.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  5. Picomolar fluorescent probes for compound affinity determination to carbonic anhydrase IX expressed in live cancer cells
    Authors: J Matulien?, G Žvinys, V Petrauskas, A Kvietkausk, A Zakšauskas, K Shubin, A Zubrien?, L Baranauski, L Ka?enauska, S Kopanchuk, S Veiksina, V Paketuryt?, J Smirnovien, V Juozapaiti, A Mickevi?i?, V Michailovi, J Jachno, D Stravinski, A Sližien?, A Petroši?t?, HM Becker, J Kazokait?-, A Yaromina, E ?apkauskai, A Rinken, V Dudutien?, LJ Dubois, D Matulis
    Scientific Reports, 2022-10-21;12(1):17644.
    Species: Human
    Sample Types: Whole Cell
    Applications: Flow Cytometry
  6. BDK inhibition acts as a catabolic switch to mimic fasting and improve metabolism in mice
    Authors: E Bollinger, M Peloquin, J Libera, B Albuquerqu, E Pashos, A Shipstone, A Hadjipanay, Z Sun, G Xing, M Clasquin, JC Stansfield, B Tierney, S Gernhardt, CP Siddall, T Greizer, FJ Geoly, SR Vargas, LC Gao, G Williams, M Marshall, A Rosado, C Steppan, KJ Filipski, BB Zhang, RA Miller, RJ Roth Flach
    Molecular Metabolism, 2022-10-08;66(0):101611.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Control
  7. Impact of delta9-Tetrahydrocannabinol on Rheumatoid Arthritis Synovial Fibroblasts Alone and in Co-Culture with Peripheral Blood Mononuclear Cells
    Authors: T Lowin, C Kok, S Smutny, G Pongratz
    Biomedicines, 2022-05-11;10(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  8. In vivo overexpression of frataxin causes toxicity mediated by iron-sulfur cluster deficiency
    Authors: Claudia Huichalaf, Tyler L. Perfitt, Anna Kuperman, Renea Gooch, Ramesh C. Kovi, Karrie A. Brenneman et al.
    Molecular Therapy - Methods & Clinical Development
  9. Circulating ACE2-expressing extracellular vesicles block broad strains of SARS-CoV-2
    Authors: L El-Shennaw, AD Hoffmann, NK Dashzeveg, KM McAndrews, PJ Mehl, D Cornish, Z Yu, VL Tokars, V Nicolaescu, A Tomatsidou, C Mao, CJ Felicelli, CF Tsai, C Ostiguin, Y Jia, L Li, K Furlong, J Wysocki, X Luo, CF Ruivo, D Batlle, TJ Hope, Y Shen, YK Chae, H Zhang, VS LeBleu, T Shi, S Swaminatha, Y Luo, D Missiakas, GC Randall, AR Demonbreun, MG Ison, R Kalluri, D Fang, H Liu
    Nature Communications, 2022-01-20;13(1):405.
    Species: Human
    Sample Types: Whole Cells, Extracellular Vesicles
    Applications: Immunogold Electron Microscopy, Flow Cytometry
  10. Belantamab Mafodotin (GSK2857916) Drives Immunogenic Cell Death and Immune-mediated Antitumor Responses In Vivo
    Authors: Montes de Oca R, Alavi AS, Vitali N et al.
    Molecular Cancer Therapeutics
  11. SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2
    Authors: Qiao Lu, Jia Liu, Shuai Zhao, Maria Florencia Gomez Castro, Maudry Laurent-Rolle, Jianbo Dong et al.
    Immunity
  12. Application of small molecule FPR1 antagonists in the treatment of cancers
    Authors: DS Ahmet, HA Basheer, A Salem, D Lu, A Aghamohamm, P Weyerhäuse, A Bordiga, J Almeniawi, S Rashid, PA Cooper, SD Shnyder, V Vinader, K Afarinkia
    Sci Rep, 2020-10-14;10(1):17249.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Acetyl-CoA Carboxylase inhibition improves multiple dimensions of NASH pathogenesis in model systems
    Authors: TT Ross, C Crowley, KL Kelly, A Rinaldi, DA Beebe, MP Lech, RV Martinez, S Carvajal-G, M Boucher, D Hirenallur, J Morin, AC Opsahl, SR Vargas, KK Bence, JA Pfefferkor, WP Esler
    Cell Mol Gastroenterol Hepatol, 2020-06-09;0(0):.
    Species: Rat
    Sample Types: Whole Tissues
    Applications: IHC
  14. SPOP Promotes Nanog Destruction to Suppress Stem Cell Traits and Prostate Cancer Progression
    Authors: Jinfang Zhang, Ming Chen, Yasheng Zhu, Xiangpeng Dai, Fabin Dang, Junming Ren et al.
    Developmental Cell
  15. Targeting of cadherin-11 decreases skin fibrosis in the tight skin-1 mouse model
    Authors: M Pedroza, RL Welschhans, SK Agarwal
    PLoS ONE, 2017-11-07;12(11):e0187109.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Ror2 Signaling and Its Relevance in Breast Cancer Progression
    Authors: Michaela Bayerlová, Kerstin Menck, Florian Klemm, Alexander Wolff, Tobias Pukrop, Claudia Binder et al.
    Frontiers in Oncology

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Reviews for Mouse IgG2A Alexa Fluor® 488-conjugated Antibody

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Mouse IgG2A Alexa Fluor® 488-conjugated Antibody
By Anonymous on 02/02/2023
Application: Flow Sample Tested: Human tissue Species: Human

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Flow Cytometry Mouse IgG2A Alexa Fluor® 488-conjugated Antibody IC003G