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Viral Japanese Encephalitis virus NS1 Antibody

Catalog #: MAB100062 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB100062-100
MAB100062-SP
Viral Japanese Encephalitis virus NS1 ELISA Standard Curve.
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Viral Japanese Encephalitis virus NS1 Antibody Summary

Species Reactivity
Viral
Specificity
Detects viral Japanese Encephalitis virus NS1 protein in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 995026
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell, HEK293-derived viral Japanese Encephalitis virus NS1 protein
Thr796-Ala1146
Accession # P32886
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Viral Japanese Encephalitis virus NS1 Monoclonal Antibody (Catalog # MAB100061).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA Viral Japanese Encephalitis virus NS1 Antibody in ELISA Standard Curve. View Larger

Viral Japanese Encephalitis virus NS1 ELISA Standard Curve. Recombinant Viral Japanese Encephalitis virus NS1 protein was serially diluted 2-fold and captured by Mouse Anti-Viral Japanese Encephalitis virus NS1 Monoclonal Antibody (Catalog # MAB100062) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral Japanese Encephalitis virus NS1 Monoclonal Antibody (Catalog # MAB100061) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Japanese Encephalitis virus NS1

Japanese encephalitis virus (JEV) non-structural protein 1 (NS1) is produced by the Japanese encephalitis virus (JEV), a mosquito-borne flavivirus that is the leading cause of virus-induced encephalitis globally (1). Flavivirus NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (2). The secreted hexamer NS1 is involved in immune evasion and pathogenesis; it is identified as a potential diagnostic marker for early detection of the virus infections (2). Mature viral JEV NS1 contains 352 amino acids and shares high structural similarity to other flavivirus NS1 proteins, such as DENV and ZIKV.

References
  1. Nain, M. et al. (2016) Rev. Med. Virol. 26:129.
  2. Rastogi, M. et al. (2016) Virol. J. 13:131.
Entrez Gene IDs
1489713 (Viral)
Alternate Names
Japanese Encephalitis virus NS1; Japanese Encephalitis; JE virus; JEV; JEVgp1; JEV-NS1

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