Home / IgG / Human IgG Antibody

Human IgG Antibody

Catalog #: MAB11012 Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB11012-100
MAB11012-SP
Human IgG1ELISA Standard Curve.
4 Images
Product Details
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IgG Antibody Summary

Species Reactivity
Human
Specificity
Detects human IgG in direct ELISAs. As a pan-Human IgG-specific antibody, sandwich immunoassay specificity is determined by the capture antibody.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1268C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Purified IgG from human serum
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as a Human IgG ELISA detection antibody when paired with Mouse Anti-Human IgG1 Monoclonal Antibody (Catalog # MAB9894), Rabbit Anti-Human IgG1 Monoclonal Antibody (Catalog # MAB9930), Mouse Anti-Human IgG2 Monoclonal Antibody (Catalog # MAB9794), Rabbit Anti-Human IgG2 Monoclonal Antibody (Catalog # MAB97941), Mouse Anti-Human IgG4 Monoclonal Antibody (Catalog # MAB9895), Rabbit Anti-Human IgG4 Monoclonal Antibody (Catalog # MAB98951), or Human IgG Monoclonal Antibody (Catalog # MAB11013).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA Human IgG<sub>1</sub>ELISA Standard Curve. View Larger

Human IgG1ELISA Standard Curve. Recombinant Human IgG1protein was serially diluted 2-fold and captured by Mouse Anti-Human IgG1Monoclonal Antibody (Catalog # MAB9894) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

ELISA Human IgG<sub>2</sub>ELISA Standard Curve. View Larger

Human IgG2ELISA Standard Curve. Recombinant Human IgG2protein was serially diluted 2-fold and captured by Mouse Anti-Human IgG2Monoclonal Antibody (Catalog # MAB9794) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

ELISA Human IgG<sub>4</sub>ELISA Standard Curve. View Larger

Human IgG4ELISA Standard Curve. Recombinant Human IgG4protein was serially diluted 2-fold and captured by Mouse Anti-Human IgG4Monoclonal Antibody (Catalog # MAB9895) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

ELISA Human IgG Antibody in ELISA Standard Curve. View Larger

Human IgG ELISA Standard Curve. Recombinant Human IgG protein was serially diluted 2-fold and captured by Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11013) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog #MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

FAQs

  1. Do these antibodies (MAB11012, F0157, F0156, and F0158) recognize IgG3?

    • This clone was raised against purified IgG from Human Serum. It was tested and shown to recognize Human IgG1. It is expected to recognize all subclasses of Human IgG, but other subclasses have not been tested.  

View all Antibody FAQs
Loading...

Reviews for Human IgG Antibody

There are currently no reviews for this product. Be the first to review Human IgG Antibody and earn rewards!

Have you used Human IgG Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review