Human IGFBP-rp1/IGFBP-7 Antibody

Catalog #: MAB13342 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB13342-100
MAB13342-SP

Human IGFBP‑rp1/IGFBP‑7 ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IGFBP-rp1/IGFBP-7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IGFBP‑rp1/IGFBP-7 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 192535

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IGFBP‑rp1/IGFBP-7
Asp30-Arg277
Accession # AAA16187

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human IGFBP‑rp1/IGFBP‑7 Monoclonal Antibody (Catalog # MAB1334).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IGFBP-rp1/IGFBP-7 DuoSet ELISA Kit (Catalog # DY1334-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human IGFBP‑rp1/IGFBP‑7 ELISA Standard Curve. Recombinant Human IGFBP-rp1/IGFBP-7 protein was serially diluted 2-fold and captured by Mouse Anti-Human IGFBP-rp1/IGFBP-7 Monoclonal Antibody (Catalog # MAB13342) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IGFBP-rp1/IGFBP-7 Monoclonal Antibody (Catalog # MAB1334) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-rp1/IGFBP-7

IGFBP-rp1, also known as Mac25/Angiomodulin (AGM), tumor-derived adhesion factor (TAF) and prostacyclin-stimulating factor (PSF), is a secreted protein that contains three protein domain modules. Human IGFBP-rp1 cDNA encodes 282 amino acid (aa) precursor protein with a putative 26 aa signal peptide. Mature
IGFBP-rp1 is a glycosylated protein with an N-terminal IGFBP domain, followed by a Kazal-type serine proteinase inhibitor domain and a C-terminal
immunoglobulin‑like C2-type domain. The similarity of IGFBP-rp1 with the IGFBPs is confined to the N-terminal IGFBP domain, which contains all 12 of the conserved cysteine residues found in IGFBP1 through 5. Human and mouse IGFBP-rP1 are highly homologous. Human and mouse IGFBP-rp1 share 94% aa sequence identity. IGFBP-rp1 is expressed in many normal tissues and in cancer cells. It is abundantly expressed in high endothelial venules (HEVs) of blood vessels in the secondary lymphoid tissues. The expression of IGFBP-rp1 is upregulated in senescing epithelial cells and by retinoic acid. IGFBP-rp1 binds IGF and insulin with very low affinity and has been shown to enhance the mitogenic actions of IGF and insulin. IGFBP-rp1 also has IGF/insulin-independent activities. It interacts with heparan sulfate proteoglycans, type IV collagen, and specific chemokines. IGFBP‑rp1 supports weak cell adhesion, promotes cell spreading on type IV collagen, and stimulates the production of the potent vasodilator PGI₂. It modulates tumor cell growth and has also been implicated in angiogenesis. IGFBP-rp1 is proteolytically cleaved between lysine 97 and alanine 98. Cleaved IGFBP-rp1 has enhanced cell attachment activity but can no longer bind IGF/insulin (1-3).

References

Hwa, V. et al. (1999) Endocrinology Rev. 20:761.
Nagakubo, D. et al. (2003) J. Immunol. 171:553.
Ahmed, S. et al. (2003) Biochem. Biophys. Res. Commun. 310:612.

Long Name

Insulin-like Growth Factor Binding Protein Related Protein 1

Entrez Gene IDs

3490 (Human); 29817 (Mouse)

Alternate Names

angiomodulin; FSTL2; IBP-7; IGFBP7; IGFBP-7; IGFBP-7PGI2-stimulating factor; IGFBP-7v; IGFBPrp1; IGFBP-rp1; insulin-like growth factor binding protein 7; insulin-like growth factor-binding protein 7; MAC25 protein; Mac25; MAC25IGF-binding protein 7; Prostacyclin-stimulating factor; PSF; PSFAGM; TAF; Tumor-derived adhesion factor