Human ADAM10 Antibody

Catalog #: MAB14272 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB14272-100
MAB14272-SP

All ADAM10 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ADAM10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAM10 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 172011

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Spodoptera frugiperda, insect ovarian cell line Sf 21 (baculovirus)-derived human ADAM10 protein
Thr214-Glu672
Accession # O14672

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human ADAM10 Monoclonal Antibody (Catalog # MAB14271).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM10

ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).

References

Rooke, et al. (1996) Science 273:1227.
Pan and Rubin (1997) Cell 90:271.
Qi, et al. (1999) Science 283:91.
Lammich, et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
Rosendahl, et al. (1997) J. Biol. Chem. 272:24588.
Chantry, et al. (1989) J. Biol. Chem. 264:21603.
Millichip, et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
Chantry and Glynn (1990) Biochem. J. 268:245.
Fahrenholz, et al. (2000) Ann. N.Y. Acad. Sci. 920:215.

Long Name

A Disintegrin and Metalloprotease-like Domain 10

Entrez Gene IDs

102 (Human); 11487 (Mouse)

Alternate Names

a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10; AD10; ADAM 10; ADAM metallopeptidase domain 10; ADAM10; CD156c antigen; CD156c; CDw156; disintegrin and metalloproteinase domain-containing protein 10; EC 3.4.24; HsT18717; kuz; Kuzbanian protein homolog; Kuzbanian; MADM; MADMEC 3.4.24.81; Mammalian disintegrin-metalloprotease