Human MIS/AMH Antibody

Catalog #: MAB17374 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB17374-100
MAB17374-SP

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Human MIS/AMH Antibody Summary

Species Reactivity

Human

Specificity

Detects human MIS/AMH in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 213828

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MIS/AMH
Ala453-Arg560
Accession # NP_000470

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human MIS/AMH Monoclonal Antibody (Catalog # MAB17373).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human MIS/AMH DuoSet ELISA Kit (Catalog # DY1737) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human MIS/AMH ELISA Standard Curve. Recombinant Human MIS/AMH protein was serially diluted 2-fold and captured by Mouse Anti-Human MIS/AMH Monoclonal Antibody (Catalog # MAB17374) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human MIS/AMH Monoclonal Antibody (Catalog # MAB17373) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MIS/AMH

Müllerian inhibiting substance (MIS), also named anti-Müllerian hormone (AMH), is a tissue-specific TGF-beta superfamily growth factor. Its expression is restricted to the Sertoli cells of fetal and postnatal testis, and to the granulosa cells of postnatal ovary (1). The human MIS gene encodes a 553 amino acid residue (aa) prepropeptide containing a signal a sequence (1‑24), a pro-region (25‑455), and the carboxyl-terminal bioactive protein (446-553) (2-4). MIS is synthesized and secreted as a disulfide-linked homodimeric pro-protein. Proteolytic cleavage is required to generate the N-terminal pro-region and the C-terminal bioactive protein, which remain associated in a non-covalent complex. Recombinant C-terminal MIS has been shown to be bioactive. However, the complex with the N-terminal pro-region showed enhanced activity (3, 5). The C-terminal region contains the seven canonical cysteine residues found in TGF-beta superfamily members. These cysteine residues are involved in inter- and intra-molecular disulfide bonds, which forms the cysteine knot structure. Human and mouse MIS share 73% and 90% aa sequence identity in their pro-region and C-terminal region, respectively. MIS induces Mullerian duct (female reproductive tract) regression during sexual differentiation in the male embryo (6). Posnatally, MIS has been shown to regulate gonadal functions (1). MIS inhibits Leydig cell proliferation and is a regulator of the initial and cyclic recruitment of ovarian follicles. MIS has also been found to have anti-proliferative effects on breast, ovarian and prostate tumor cells (7-9).

Like other TGF-beta superfamily members, MIS signals via a heteromeric receptor complex consisting of a type I and a type II receptor serine/threonine kinase. Depending on the cell context, different type I receptors (including Act RIA/ALK2, BMP RIA/ALK3, and BMP RIB/ALK6) that are shared by other TGF-beta superfamily members, have been implicated in MIS signaling (10-12). In contrast, the type II MIS receptor (MIS RII) is unique and does not bind other TGF-beta superfamily members. Upon ligand binding, MIS RII recruits the non-ligand binding type I receptor into the complex, resulting in phosphorylation the BMP-like signaling pathway effector proteins Smad1, Smad5 and Smad 8 (10-12).

References

Teixeira, et al. (2001) Endocrine Rev. 22:657.
Pepinsky, R.et al. (1988) J. Biol. Chem. 263:18961.
Wilson, C.A. et al. (1993) Mol. Endocrinol. 7:247.
Kurian, M.S. et al. (1995) Clin. Cancer Res. 1:343.
Nachtigal, J.S. and H.A. Ingraham (1996) Proc. Natl. Acad. Sci. USA 93:7711.
MacLaughlin, D.T. et al. (1991) Methods Enzymol. 35:358.
Laurich, V.M. et al. (2002) Endocrinology 143:3351.
McGee, E.A. et al. (2001) Biol. Reprod. 64:293.
Segev, D.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:239.
Josso, N and N. diClemente (2003) Trends Endo. Met. 14:91.
Clarke, T.R. et al. (2001) Mol. Endocrinol. 15:946.
Visser, J.A. (2003) Mol. Cell. Endocrinol. 211:65.

Long Name

Mullerian-inhibiting Substance/Anti-Mullerian Hormone

Entrez Gene IDs

268 (Human); 11705 (Mouse)

Alternate Names

AMH; MIF; MIS; Muellerian hormone; muellerian-inhibiting factor; muellerian-inhibiting substance; Mullerian hormone; Mullerian inhibiting factor; Mullerian inhibiting substance