Human GDF-11/GDF-8 Antibody

Catalog #: MAB19582R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB19582R-100
MAB19582R-SP

Induction of Hemoglobin Expression by GDF-11/BMP-11 and Neutralization by anti-GDF-11/GDF-8 Antibody.

2 Images

All GDF-11/GDF-8 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GDF-11/GDF-8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GDF-11/GDF-8 in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 743835R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human GDF-11/GDF-8
Asn299-Ser407
Accession # O95390

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human GDF‑11/BMP‑11 Monoclonal Antibody (Catalog # MAB19584).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human GDF-11/BMP-11 DuoSet ELISA Kit (Catalog # DY1958-05) for convenient development of a sandwich ELISA.

Neutralization

Measured by its ability to neutralize GDF‑11/BMP‑11 induction of hemoglobin expression in the K562 human chronic myelogenous leukemia cell line, Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The Neutralization Dose (ND₅₀) is typically 0.04-0.2 μg/mL in the presence of 25 ng/mL Recombinant Human GDF‑11/BMP‑11. This antibody also neutralizes GDF-8 in a similar K562 bioassay.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

View Larger

Induction of Hemoglobin Expression by GDF-11/BMP-11 and Neutralization by anti-GDF-11/GDF-8 Antibody. Recombinant Human GDF-11/BMP-11 (Catalog # 1958-GD) induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the pseudoperoxidase assay. Induction of hemoglobin expression by GDF-11/BMP-11 (25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human GDF-11/GDF-8 Monoclonal Antibody (Catalog # MAB19582R). The ND₅₀ is typically 0.04-0.2 µg/mL. This antibody also neutralizes GDF-8 in a similar K562 bioassay.

ELISA

View Larger

Human GDF‑11/BMP‑11 ELISA Standard Curve. Recombinant Human GDF-11/BMP-11 protein was serially diluted 2-fold and captured by Mouse Anti-Human GDF-11/BMP-11 Monoclonal Antibody (Catalog # MAB19584) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GDF-11/GDF-8 Monoclonal Antibody (Catalog # MAB19582R) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-11/GDF-8

Growth Differentiation Factor 11 (GDF-11), also known as BMP-11, is a member of the TGF-beta superfamily and is highly related to GDF-8. GDF-11 encodes a 407 amino acid (aa) prepropeptide processed into a 109 aa mature protein. Mature GDF-11 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members; however, like the TGF-beta s, Activins and GDF-8, GDF-11 also contains one extra pair of cysteine residues. At the amino acid sequence level, mature human, mouse, rat and chicken GDF-11 are 99-100% identical. Mature GDF-11 and GDF-8 share 90% amino acid sequence identity. GDF-11 is expressed in diverse regions of the mouse embryo: tailbud, somitic precursors, limbs, mandibular and branchial arches, dorsal neural tube, odontoblasts, nasal epithelium, and particular regions of the brain (1). GDF-11 signals through the Activin type II receptors and induces phosphorylation of Smad2 to mediate axial patterning (2). Systemic GDF-11 levels decline with age and administration of higher levels of GDF-11 can reverse age-related cardiac hypertrophy (3). In addition, systemic administration of recombinant GDF-11 protein restores genomic integrity and health of muscle stem cells, neurovasculature and enhances neurogenesis (4, 5).

References

Nakashima, M. et al. (1999) Mech. Dev. 80:185.
Oh, S.P. et al. (2002) Genes & Dev. 16:274.
Loffredo, F.S. et. al. (2013) Cell. 153.828.
Katsimpardi, L. et. al. (2014) Science. 344.630.
Sinha, M. et. al. (2014) Science. 344.649.

Long Name

Growth Differentiation Factor 11/8

Entrez Gene IDs

10220 (Human)

Alternate Names

BMP11; BMP-11; bone morphogenetic protein 11; GDF-11; GDF-11/GDF-8; growth/differentiation factor 11; VHO

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human GDF-11/GDF-8 Antibody

There are currently no reviews for this product. Be the first to review Human GDF-11/GDF-8 Antibody and earn rewards!

Have you used Human GDF-11/GDF-8 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review