Human IL-38/IL-1F10 Antibody

Catalog #: MAB24271 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB24271-100
MAB24271-SP

Human IL‑38/IL‑1F10 ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-38/IL-1F10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑38/IL‑1F10 in direct ELISAs.

Source

Monoclonal Mouse IgG₃ Clone # 892257

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived human IL‑38/IL‑1F10
Ser3-Trp152
Accession # Q8WWZ1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human IL‑38/IL‑1F10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2427).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IL-38/IL-1F10 DuoSet ELISA Kit (Catalog # DY9110-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human IL‑38/IL‑1F10 ELISA Standard Curve. Recombinant Human IL-38/IL-1F10 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB24271) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-38/IL-1F10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2427) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-38/IL-1F10

Human IL-38, previously called IL-1F10 or IL-1HY2, is a secreted cytokine belonging to the IL-1 family. It is synthesized as a 152 amino acid mature protein that lacks an identifiable signal sequence. It binds to soluble IL-1 RI. Human IL-38/IL-1F10 shares 82% aa sequence identity with mouse IL-38/IL-1F10. Human IL-38/IL-1F10 also shares 39% and 27% aa sequence identity with IL-1ra/IL-1F3 and IL-1 beta /IL-1F2, respectively.

Long Name

Interleukin 1 Family Member 10

Entrez Gene IDs

84639 (Human); 215274 (Mouse); 102125744 (Cynomolgus Monkey)

Alternate Names

FIL1- theta; FKSG75; IL1F10; IL-1HY2; IL38; IL-38; interleukin 1 family, member 10 (theta); UNQ6119/PRO20041